A5044519058- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Virology and Viral Diseases
- Biotechnology and Related Fields
- Bacillus and Francisella bacterial research
- Parasitic Infections and Diagnostics
- Cytomegalovirus and herpesvirus research
- Viral Infections and Outbreaks Research
- Microbial infections and disease research
- Research on Leishmaniasis Studies
- Viral-associated cancers and disorders
- Virus-based gene therapy research
- Dermatological diseases and infestations
- T-cell and Retrovirus Studies
- Insect symbiosis and bacterial influences
- Biomedical and Engineering Education
- Insect and Pesticide Research
- Pancreatitis Pathology and Treatment
- Turtle Biology and Conservation
- Poxvirus research and outbreaks
University of Surrey
2017
The Pirbright Institute
2007
UConn Health
2001-2003
University of Zurich
1998-2002
Institute of Virology of the Slovak Academy of Sciences
1998
Four poll Dorset sheep and four Holstein‐Friesian cattle were infected with the northern European strain of bluetongue virus ( btv ), btv‐8 , to assess its pathogenicity in uk breeds. The time course infection was monitored both species by using real‐time reverse transcriptase‐ pcr rt‐pcr conventional serology. Two developed severe clinical signs that would have been fatal field; other two moderately mildly ill, respectively. clinically unaffected, but had high levels viral rna their...
An earlier competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) was developed for detection of specific antibody against malignant catarrhal fever (MCF) viruses (MCFV) in ruminants. In this study, the indirect CI-ELISA improved by conjugating monoclonal 15-A directly with horseradish peroxidase and developing a method producing precoated, dried antigen plates. This new test is referred to as direct CI-ELISA. The reformatted yielded significantly sensitivity, time required...
ABSTRACT Until recently, sheep-associated malignant catarrhal fever (SA-MCF) was diagnosed mainly on the basis of clinical presentation and histopathological changes. Using clinically field cases, we have evaluated a seminested PCR competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) compared these assays in diagnosis SA-MCF cattle with histopathology as provisional “gold standard.” Samples from 44 signs suggestive were examined by histopathology, PCR, CI-ELISA. In addition,...
A total of 486 serum samples collected from several species both free-living and captive cervids in Germany was examined for antibodies against malignant catarrhal fever (MCF)-associated viruses (MCFV) by a competitive-inhibition enzyme-linked immunosorbent assay (CI-ELISA). Eleven (2%) these were positive MCFV. Among 157 16 different deer including four (7%) 54 fallow one 14 sika (Cervus nippon) seropositive. 329 three free-ranging deer, 253 roe (Capreolus capreolus), 22 red elaphus) dama),...
SUMMARY Effects of tick feeding on an early antigen‐specific T cell response were studied by monitoring a clonotypic population adoptively transferred receptor (TCR) transgenic CD4 cells responding to tick‐associated antigen. When recipient mice infested with pathogen‐free Ixodes scapularis nymphs several days prior transfer and intradermal injection soluble cognate antigen at the site, gained ability express Th2 effector cytokine IL‐4. Notably, this effect was not only observed in BALB/c...
SUMMARY The gene encoding a 36‐kDa (p36) immunomodulatory protein present in saliva of Dermacentor andersoni was cloned prokaryotic and eukaryotic expression vectors. A polymerase chain reaction (PCR)‐generated cDNA lacking signal peptide into the Escherichia coli vector pET28 similar sequence pIB/V5‐His‐TOPO for stable transfection insect cells, High 5 ™ . 26‐kDa molecular mass p36 expressed by bacteria is agreement with that predicted from translated full‐length sequence....
Borna Disease (BD) is a mostly fatal disease of horses and sheep endemic in central Europe. Antibodies to virus (BDV) have been described other species living BD non-endemic areas. Meaningful clinical BDV serology hampered by difficulties defining serological cut-offs, which require the investigation populations from Here we studied areas similar geography Switzerland. antigens were detected ELISA indirect immunofluorescence analysis (IFA) only sera 3 6 with autopsy confirmed BD. One serum...
Background: Leishmaniasis is a major tropical and subtropical parasitic disease.Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, ketoconazole are drugs used to treat this disease.Some of these cause severe adverse side effects treatment failures common.Allopurinol, purine analog, has been leishmaniasis, alone or combined with the previously mentioned drugs.Low cost, ease administration (oral), lack toxicity make allopurinol particularly appealing...