A5022888668- Microbial infections and disease research
- Blood groups and transfusion
- Reproductive tract infections research
- Escherichia coli research studies
- Urinary Tract Infections Management
- Streptococcal Infections and Treatments
- Gut microbiota and health
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Toxoplasma gondii Research Studies
- Plant and Fungal Interactions Research
- Bacterial biofilms and quorum sensing
- Toxin Mechanisms and Immunotoxins
- Pharmacological Effects of Natural Compounds
- Biochemical and Structural Characterization
- Mosquito-borne diseases and control
- Antibiotic Resistance in Bacteria
- Plant Pathogens and Resistance
- Bartonella species infections research
- Viral gastroenteritis research and epidemiology
- Plant-based Medicinal Research
- Animal Virus Infections Studies
- Parasitic Infections and Diagnostics
- Aquaculture disease management and microbiota
University of Bern
2024-2025
University of Southern Denmark
2021-2022
Universitat Autònoma de Barcelona
2015-2021
Abstract The Mycoplasma Immunoglobulin Binding/Protease (MIB-MIP) system is a candidate ‘virulence factor present in multiple pathogenic species of the Mollicutes , including fast-growing feriruminatoris . MIB-MIP cleaves heavy chain host immunoglobulins, hence affecting antigen-antibody interactions and potentially facilitating immune evasion. In this work, using -omics technologies 5’RACE, we show that four copies M. have different expression levels are transcribed as operons controlled by...
<title>Abstract</title> Extracellular vesicles (EVs) are central components of bacterial secretomes, including the small, cell wall-less <italic>Mollicutes</italic>. Although EV release in <italic>Mollicutes </italic>has been reported, proteomic composition and function have not explored yet. We developed a protocol for isolating EVs pathogens <italic>Mycoplasma mycoides</italic>subsp. <italic>capri</italic> (<italic>Mmc</italic>)...
Adhesion of pathogenic bacteria to target cells is a prerequisite for colonization and further infection. The main adhesins the emerging sexually transmitted pathogen Mycoplasma genitalium, P140 P110, interact form Nap complex anchored cell membrane. Herein, we present crystal structures extracellular region virulence factor P110 (916 residues) unliganded in with sialic acid oligosaccharides. interacts only neuraminic moiety oligosaccharides experiments human demonstrate that these...
The Mycoplasma genitalium MG428 protein shows homology to members of the sigma-70 family sigma factors. Herein, we found that activates transcription recA, ruvA and ruvB as well several genes with unknown function. Deletion MG_428 or some up-regulated led severe recombination defects. Single cell analyses revealed activation MG428-regulon is a rare event under laboratory growth conditions. A conserved sequence promoter architecture (TTGTCA-N18/19-ATTWAT) was identified in upstream region all...
Uropathogenic Escherichia coli (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little known on key fitness-factors growth in lab media and UTI. Here, we transposon-insertion-sequencing approach (TraDIS) reveal the essential-genes for vitro fitness-gene-sets Luria broth (LB) EZ-MOPS medium without glucose, as well human bacteriuria mouse cystitis. A total of 293 essential genes were identified set...
Most uropathogenic Escherichia coli (UPEC) express type-1 fimbriae (T1F), a key virulence factor for urinary tract infection (UTI) in mice. Evidence that conclusively associates this pilus with uropathogenesis humans has, however, been difficult to obtain. We used an experimental porcine model of cystitis assess the role T1F larger mammals more closely related humans. Thirty-one pigs were infected UPEC strain UTI89 or its deficient mutant, UTI89ΔfimH, at inoculum titres 102 108 colony...
Emergence of antibiotic resistant bacteria is evolving at an alarming pace; therefore, we must start turning to alternative approaches. One these, could be the use adjuvants that enhances effect antibiotics towards bacteria. A novel adjuvant cannabidiol (CBD), which have previously shown can enhance bacitracin (BAC). BAC targets cell wall synthesis by inhibiting dephosphorylation lipid carrier undecaprenyl pyrophosphate prior recycling across membrane. However, mechanism underlying this CBD...
Abstract Mycoplasma genitalium is a human pathogen adhering to host target epithelial cells and causing urethritis, cervicitis pelvic inflammatory disease. Essential for infectivity transmembrane adhesion complex called Nap comprising proteins P110 P140. Here we report the crystal structure of P140 both alone in with N-terminal domain P110. By cryo-electron microscopy (cryo-EM) tomography (cryo-ET) find closed open conformations, determined at 9.8 15 Å, respectively. Both structures cryo-EM...
It is well-established that FtsZ drives peptidoglycan synthesis at the division site in walled bacteria. However, function and conservation of wall-less prokaryotes such as mycoplasmas are less clear. In genome-reduced bacterium Mycoplasma genitalium , cell gene cluster limited to four genes: mraZ mraW MG_223, ftsZ . a previous study, we demonstrated was dispensable for growth M. under laboratory culture conditions. Herein, show entire non-essential vitro Our analyses indicate loss alone...
In the human pathogen Mycoplasma genitalium, homologous recombination is under control of σ20, an alternative sigma factor that boosts generation genetic and antigenic diversity in population. Under laboratory growth conditions, σ20 activation rare factors governing its intermittent activity are unknown. Two σ20-regulated genes, rrlA rrlB, showed to be important for DNA sequences this bacterium. Herein, we demonstrate rrlB code two small proteins participate a feed-forward loop essential...
Animal shelters, especially in resource-poor countries, bring together pets from different regions and with backgrounds. The crowding of such animals often results infectious diseases, as respiratory infections. This study characterized
Transition metals participate in numerous enzymatic reactions and they are essential for survival all living organisms. For this reason, bacterial pathogens have evolved dedicated machineries to effectively compete with their hosts scavenge at the site of infection. In study, we investigated mechanisms controlling metal acquisition emerging human pathogen Mycoplasma genitalium. We observed a robust transcriptional response starvation, many genes coding predicted lipoproteins ABC-transporters...
Wesselsbron virus (WSLV) is a zoonotic, mosquito-borne orthoflavivirus endemic to sub-Saharan Africa, causing abortions and stillbirths in small ruminants. The life cycle of WSLV involves Aedes mosquitoes various wildlife domestic animals. Seminal studies the 1950s have shown zoonotic potential WSLV, notably accidental infections laboratory workers exposed infected material. More recent epidemiological suggest emergence clade I strains peri-domestic rural areas western eastern Africa....
<title>Abstract</title> The Mycoplasma Immunoglobulin Binding/Protease (MIB-MIP) system is a candidate virulence factor present in multiple pathogenic species of the <italic>Mollicutes</italic>, including fast-growing <italic>Mycoplasma feriruminatoris</italic>. MIB-MIP cleaves heavy chain host immunoglobulins, hence affecting antigen-antibody interactions and potentially facilitating immune evasion. In this work we analyzed distribution genetic relatedness between systems different...