A5065533025- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- T-cell and Retrovirus Studies
- Vector-borne infectious diseases
- Aquaculture disease management and microbiota
- Immune Response and Inflammation
- Transgenic Plants and Applications
- Vibrio bacteria research studies
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Slime Mold and Myxomycetes Research
- Biochemical and Structural Characterization
- Influenza Virus Research Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
- Carbohydrate Chemistry and Synthesis
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Rabies epidemiology and control
- Stochastic processes and statistical mechanics
- Nursing Diagnosis and Documentation
- Virology and Viral Diseases
International Livestock Research Institute
2014-2024
Washington State University
2024
Centre de Recerca en Sanitat Animal
2011-2018
Universitat Autònoma de Barcelona
2012-2017
Universitat de Barcelona
2008-2009
Laboratoire de physique des Solides
2001
Université Paris-Sud
2001
African swine fever is a highly contagious viral disease of mandatory declaration to the World Organization for Animal Health (OIE). The lack available vaccines makes its control difficult; thus, virus (ASFV) represents major threat industry. Inactivated do not confer solid protection against ASFV. Conversely, live attenuated viruses (LAV), either naturally isolated or obtained by genetic manipulation, have demonstrated reliable homologous ASFV strains, although little no has been...
The lack of available vaccines against African swine fever virus (ASFV) means that the evaluation new immunization strategies is required. Here we show fusion extracellular domain ASFV Hemagglutinin (sHA) to p54 and p30, two immunodominant structural viral antigens, exponentially improved both humoral cellular responses induced in pigs after DNA immunization. However, with resulting plasmid (pCMV-sHAPQ) did not confer protection lethal challenge virulent E75 ASFV-strain. Due fact CD8(+)...
African swine fever is one of the most devastating pig diseases, against which there no vaccine available. Recent work from our laboratory has demonstrated protective potential DNA vaccines encoding three viral antigens (p54, p30, and hemagglutinin extracellular domain) fused to ubiquitin. Partial protection was afforded in absence detectable antibodies prior virus challenge, survival correlated with presence a large number hemagglutinin-specific CD8(+) T cells blood. Aiming demonstrate...
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains only completely determined. A large part of studies on function ASFV genes, viral transcription, replication, DNA repair morphogenesis, been performed using this model. This avirulent obtained by adaptation to grow Vero cells highly virulent BA71 strain. We report here analysis sequence comparison with that BA71V. They possess smallest genomes for or an...
African swine fever virus (ASFV) is the causal agent of fever, a hemorrhagic and often lethal porcine disease causing enormous economical losses in affected countries. Endemic for decades most sub-Saharan countries Sardinia, risk ASFV-endemicity Europe has increased since its last introduction into 2007. Live attenuated viruses have been demonstrated to induce very efficient protective immune responses, albeit time protection was circumscribed homologous ASFV challenges. However, their use...
Abstract Most of the microorganisms living in a symbiotic relationship different animal body sites (microbiota) reside gastrointestinal tract (GIT). Several studies have shown that microbiota is involved host susceptibilities to pathogens. The fecal domestic and wild suids was analyzed. Bacterial communities were determined from feces obtained pigs ( Sus scrofa ) raised under conditions: specific-pathogen-free (SPF) same bred, indigenous backyard farm Kenya. Secondly, composition African...
Comparison between the lipopolysaccharide (LPS) core structures of Aeromonas salmonicida subsp. A450 and hydrophila AH-3 shows great similarity in inner LPS part outer but some differences distal (residues ld-Hep, d-Gal, d-GalNAc). The three genomic regions encoding biosynthetic genes A. A450, which 2 3 have identical to those AH-3, were fully sequenced. region 1 showed seven genes: similar not one without any homology well-characterized gene. mutants with alterations that constructed, their...
We describe the characterization of an African swine fever genotype IX virus (ASFV-Kenya-IX-1033), which was isolated from a domestic pig in western Kenya during reported outbreak. This includes efficiency replication and vivo virulence, together with genome stability following passage blood macrophages wild boar lung cell line (WSL). The ASFV-Kenya-IX-1033 stock retained its ability to replicate primary virulence vivo, more than 20 passages WSL. At whole level, few single-nucleotide...
Nanoparticle vaccines usually prime stronger immune responses than soluble antigens. Within this class of subunit vaccines, the recent development computationally designed self-assembling two-component protein nanoparticle scaffolds provides a powerful and versatile platform for displaying multiple copies one or more Here we report generation three different immunogens 60 p67C, an 80 amino acid polypeptide from candidate vaccine antigen Theileria parva , their immunogenicity in cattle. p67C...
ABSTRACT By the isolation of three different Aeromonas hydrophila strain AH-3 (serotype O34) mutants with an altered lipopolysaccharide (LPS) migration in gels, genomic regions encompassing LPS core biosynthesis genes were identified and characterized. When possible, constructed using each gene from regions, containing seven, four, two (regions 1 to 3, respectively). The mutant structures elucidated by mass spectrometry, methylation analysis, comparison full structure O-antigen-lacking...
Abstract East Coast fever (ECF), caused by Theileria parva, is the most important tick-borne disease of cattle in sub-Saharan Africa. Practical disadvantages associated with currently used live-parasite vaccine could be overcome subunit vaccines. An 80-aa polypeptide derived from C-terminal portion p67, a sporozoite surface Ag and target neutralizing Abs, was focus efforts on vaccines against ECF subjected to several trials very promising results. However, vaccination regimen far optimized,...
African swine fever (ASF) is a pathology of pigs against which there no treatment or vaccine. Understanding the equilibrium between innate and adaptive protective responses immune might contribute to development strategies ASFV. Here we compare, using proteomic approach, course in vivo infection caused by two homologous strains: virulent E75 attenuated E75CV1. Our results show progressive loss proteins day 7 post-infection (pi) with E75, reflecting tissue destruction. Many signal pathways...
Worldwide, chronic obstructive pulmonary disease (COPD) is the fourth cause of death. Exacerbations have a negative impact on prognosis COPD and frequency severity these episodes are associated with higher patient mortality. first decompensation, hospital admission death in COPD. The incidence exacerbations has mainly been estimated populations patients moderate-severe requiring care. However, little known regarding epidemiology less severe forms. It therefore possible that high number forms...
Animal shelters, especially in resource-poor countries, bring together pets from different regions and with backgrounds. The crowding of such animals often results infectious diseases, as respiratory infections. This study characterized
East Coast fever (ECF) is a lymphoproliferative disease caused by the tick-transmitted protozoan parasite Theileria parva. ECF one of most serious cattle tick-borne diseases in Sub-Saharan Africa. We have previously demonstrated that three doses C-terminal part sporozoite protein p67 (p67C) adjuvanted with ISA206VG confers partial protection against at herd level. tested efficacy two this experimental vaccine, as reducing vaccination regimen would facilitate its deployment field. reconfirm...
The core lipopolysaccharide (LPS) of Aeromonas hydrophila AH-3 and salmonicida A450 is characterized by the presence pentasaccharide alpha-d-GlcN-(1-->7)-l-alpha-d-Hep-(1-->2)-l-alpha-d-Hep-(1-->3)-l-alpha-d-Hep-(1-->5)-alpha-Kdo. Previously it has been suggested that WahA protein involved in incorporation GlcN residue to outer LPS. contains two domains: a glycosyltransferase carbohydrate esterase. In this work we demonstrate independent expression domain catalyzes GlcNAc from UDP-GlcNAc...
Background: Immune correlates of protection are ideal tools to predict treatment or vaccine efficacy. However, the accuracy immune correlate and capability robustly outcome a candidate determined by performance in vitro immunoassay used. Several Theileria parva sporozoite seroneutralization assays have previously been used assess antibody functional activities; however, common limitation has need for fresh material, target cells sporozoites, operator-to-operator bias. An improved assay...
Obtaining a complete good-quality sequence and annotation for the long double-stranded DNA genome of African swine fever virus (ASFV) from next-generation sequencing (NGS) technology has proven difficult, despite increasing availability reference sequences affordability NGS. A gap analysis conducted by global research alliance (GARA) partners identified that standardized, automatic pipeline NGS was urgently needed, particularly new outbreak strains. Whilst there are several diagnostic labs...
Abstract In this study, we describe an African swine fever genotype IX virus (ASFV-Kenya-1033-IX), which was isolated from a domestic pig in Western Kenya during reported outbreak, including efficiency of replication, vivo virulence, and genome stability pulmonary alveolar macrophages (PAM) wild boar cell line (WSL). The ASFV-Kenya-1033-IX stock, underwent multiple passages WSL (more than 20), retained its ability to replicate primary it also the virulence . At genomic level, only few single...