A5087874511- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
- Cancer Research and Treatments
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Amoebic Infections and Treatments
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Viral Infections and Immunology Research
- Animal Nutrition and Physiology
- Animal Genetics and Reproduction
- Gut microbiota and health
- Platelet Disorders and Treatments
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Genetic Mapping and Diversity in Plants and Animals
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Clostridium difficile and Clostridium perfringens research
- Xenotransplantation and immune response
- Monoclonal and Polyclonal Antibodies Research
University of Córdoba
2015-2025
Instituto Maimónides de Investigación Biomédica de Córdoba
2021-2025
Biomedical Research Institute
2022-2025
Hospital General Universitario De Valencia
2020-2024
Cordoba University
2009-2022
Lallemand (France)
2021
Medicina
2016
Hospital General Universitari de Castelló
2015
Instituto de Agricultura Sostenible
2015
Peruvian University of Applied Sciences
2014
Once a list of differentially expressed genes has been identified from microarray experiment, subsequent post-analysis task is required in order to find the main biological processes associated experimental system. This paper describes two pathways analysis tools, ArrayUnlock and Ingenuity Pathways Analysis (IPA) deal with post-analyses data, context EADGENE SABRE workshop. Dataset employed this study proceeded an chicken infection performed host reactions after homologous or heterologous...
Salmonella enterica serovar Typhimurium causes enteric disease and compromises food safety. In pigs, the molecular response of intestine to S. typhimurium has been traditionally characterized by in vitro models that do not reflect actual immunological competence intestinal mucosa. this work, we performed an oral infection study obtain insight into three different sections (jejunum, ileum colon) porcine intestine. For this, samples from one-month-old infected piglets were collected during a...
African swine fever virus (ASFV) is the causal agent of fever, a hemorrhagic and often lethal porcine disease causing enormous economical losses in affected countries. Endemic for decades most sub-Saharan countries Sardinia, risk ASFV-endemicity Europe has increased since its last introduction into 2007. Live attenuated viruses have been demonstrated to induce very efficient protective immune responses, albeit time protection was circumscribed homologous ASFV challenges. However, their use...
Salmonella is a major foodborne pathogen which successfully infects animal species for human consumption such as swine. The has battery of virulence factors it uses to colonise and persist within the host. host microbiota may play role in resistance to, also be indirectly responsible from some consequences of, infection. To investigate this, we used 16S rRNA metagenomic sequencing determine changes gut pigs response infection by Typhimurium at three locations: ileum mucosa, content faeces....
SARS-CoV-2, the causative agent of present COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome, and some have been implicated facilitating infection pathogenesis through their interaction with cellular components. Among these proteins, protein ORF7a ORF7b functions are poorly understood. In this study, A549 cells were transduced to express ORF7b, respectively, explore more depth role each pathological manifestation leading COVID-19. Bioinformatic analysis integration...
Wastewater pathogen monitoring is useful for surveillance of enteric pathogens. Information about the presence Paslahepevirus balayani (HEV) and emergent Rocahepevirus ratti (RHEV) in untreated water their correlation with clinical cases scarce. Aim To longitudinally monitor HEV RHEV wastewater to evaluate possible human cases.This study was carried out city Cordoba (southern Spain) from March 2021 2023. occurrence were evaluated by PCR three sample types: i) sera patients acute hepatitis...
<h3>Objectives</h3> To assess the effectiveness and safety of baricitinib in treatment Rhupus. <h3>Methods</h3> We conducted a retrospective observational review medical records from Rheumatology Department at our hospital between 2019 2023, identifying patients diagnosed with Rhupus, who received baricitinib. The diagnosis Rhupus was assigned to met diagnostic criteria for both Rheumatoid Arthritis (RA) Systemic Lupus Erythematosus (SLE). study involves comprehensive analysis clinical...
The host response to S. Typhimurium infection can be post-transcriptionally regulated by miRNAs. In this study, we investigated the role of miR-215 using both in vivo porcine models and vitro intestinal epithelial cell lines. Several miRNAs were found dysregulated ileum during with wild-type SPI2-defective mutant strains Typhimurium, some changes being SPI2-dependent. Notably, was significantly downregulated infection. To explore its functional role, gain-of-function experiments performed...
Antiviral signaling, immune response and cell metabolism are dysregulated by SARS-CoV-2, the causative agent of COVID-19. Here, we show that SARS-CoV-2 accessory proteins ORF3a, ORF9b, ORF9c ORF10 induce a significant mitochondrial metabolic reprogramming in A549 lung epithelial cells. While induced largely overlapping transcriptomes, ORF3a distinct transcriptome, including downregulation numerous genes with critical roles function morphology. On other hand, all four ORFs altered dynamics...
A simple and sensitive method based on liquid chromatography-atmospheric pressure ionization-mass spectrometry is described for the determination of 4 benzimidazole pesticides (carbendazim, thiabendazole, benomyl, thiophanate-methyl) imidacloprid in vegetables fruits. Food samples were typically extracted with ethyl acetate to draw analytes into organic phase. No cleanup step was necessary before injection chromatographic (LC) system electrospray mass spectrometric detection. The separated a...
Tenascin‐X and tenascin‐C glycoproteins are phylogenetically conserved components of the extracellular matrix, although their specific roles remain to be determined. cDNA probes were produced from pig tenascin‐X genes used examine tissue distribution transcripts in 28 tissues Large‐White pigs, 4.5–42‐months old (called adults) 17 87‐day‐old fetuses. The hybridization Northern blots with revealed, most tissues, a complex pattern bands including major band about 13 kb, assumed correspond main...
Infected pork meat is an important source of non-typhoidal human salmonellosis. Understanding molecular mechanisms involved in disease pathogenesis for the development therapeutic and preventive strategies. Thus, hereby we study transcriptional profiles along porcine intestine during infection with Salmonella Typhimurium, as well post-transcriptional gene modulation by microRNAs (miRNA). Sixteen piglets were orally challenged S. Typhimurium. Samples from jejunum, ileum colon, collected 1, 2...
SARS-CoV-2, the cause of COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome. Their roles during infection are still not completely understood. In this study, transcriptomics analysis revealed that both WNT5A and IL11 were significantly up-regulated A549 cells expressing individual ORF6, ORF8, ORF9b or ORF9c from SARS-CoV-2 (Wuhan-Hu-1 isolate). is a member IL6 family cytokines. signaling-related genes also differentially expressed. Bioinformatics disclosed involved...
The aim of this paper was to describe and compare the methods used results obtained by participants in a joint EADGENE (European Animal Disease Genomic Network Excellence) SABRE (Cutting Edge Genomics for Sustainable Breeding) workshop focusing on post analysis microarray data. participating groups were provided with identical lists probes, including test statistics three different contrasts, normalised log-ratios each array, be as starting point interpreting affected probes. data originated...
Postweaning diarrhea (PWD) is a relevant problem associated with early weaning on pig farms. For decades, in-feed antibiotics and therapeutic zinc oxide (ZnO) have been widely used to prevent PWD in piglets. The European Union banning both strategies 2022 due antimicrobial resistance environmental contamination concerns, respectively. Understanding the effects of these products microbiome crucial for correcting potential microbial disbalances that would prompt PWD. Using shotgun sequencing,...
Post-weaning diarrhoea (PWD) is a multifactorial disease that affects piglets after weaning, contributing to productive and economic losses. Its control includes the use of in-feed prophylactic antibiotics therapeutic zinc oxide (ZnO), treatments that, since 2022, are no longer permitted in European Union due spread antimicrobial resistance genes pollution soil with heavy metals. A dysbiosis microbiota has been suggested as potential risk factor PWD onset. Understanding pig's development...
Salmonella Typhimurium infection in pigs is characterized by an intense inflammatory response early which seems to dysregulate host physiology including the gut microbiota. Both, changes and microbiome composition, must entail modifications within environment. This study analyzes metabolome infected (48 hours after infection) non-infected controls untargeted metabolomics (LC–QTOF MS/MS GC–TOF MS), supported with data (16S rRNA abundance prediction functional analyses). The metabolomic...
Abstract Background Post weaning diarrhoea (PWD) causes piglet morbidity and mortality at is a major driver for antimicrobial use worldwide. New regulations in the EU limit of in-feed antibiotics (Ab) therapeutic zinc oxide (ZnO) to prevent PWD. approaches control PWD are needed, understanding role microbiota this context key. In study, shotgun metagenome sequencing was used describe taxonomic functional evolution faecal during first two weeks post within three experimental groups, Ab, ZnO...
The enteropathogen Salmonella Typhimurium (S. Typhimurium) is the most commonly nontyphoideal serotype isolated in pig worldwide. Currently, one of main sources human infection by consumption pork meat. Therefore, prevention and control salmonellosis pigs crucial for minimizing risks to public health. aim present study was use isobaric tags relative absolute quantification (iTRAQ) explore differences response two segment porcine gut (ileum colon) along a time course 1, 2 6 days post (dpi)...