A5054787533- Digital Holography and Microscopy
- Advanced Fluorescence Microscopy Techniques
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- Electrowetting and Microfluidic Technologies
- Near-Field Optical Microscopy
- Image Processing Techniques and Applications
- Optical Coherence Tomography Applications
- Advanced Optical Imaging Technologies
- Photoacoustic and Ultrasonic Imaging
- CCD and CMOS Imaging Sensors
- Optical measurement and interference techniques
- Single-cell and spatial transcriptomics
- Advanced X-ray Imaging Techniques
- Characterization and Applications of Magnetic Nanoparticles
- Microfluidic and Capillary Electrophoresis Applications
- Orbital Angular Momentum in Optics
- Microbial Inactivation Methods
- Fluid Dynamics and Thin Films
- Advanced Electron Microscopy Techniques and Applications
- Nanofabrication and Lithography Techniques
- Neural Networks and Applications
- Fluid Dynamics and Heat Transfer
- Microencapsulation and Drying Processes
- Listeria monocytogenes in Food Safety
The Wallace H. Coulter Department of Biomedical Engineering
2019-2024
Georgia Institute of Technology
2019-2024
Universidad de Valladolid
2023-2024
Instituto de Salud Carlos III
2023-2024
Emory University
2020-2023
Centro Nacional de Microbiologia
2023
Institute of Applied Science and Intelligent Systems
2015-2021
National Research Council
2017-2021
AID Atlanta
2020
Stony Brook University
2018
Abstract The rapid development of scientific CMOS (sCMOS) technology has greatly advanced optical microscopy for biomedical research with superior sensitivity, resolution, field-of-view, and frame rates. However, sCMOS sensors, the parallel charge-voltage conversion different responsivity at each pixel induces extra readout pattern noise compared to charge-coupled devices (CCD) electron-multiplying CCD (EM-CCD) sensors. This can produce artifacts, deteriorate imaging capability, hinder...
Lab-on-a-Chip (LoC) devices are extremely promising in that they enable diagnostic functions at the point-of-care. Within this scope, an important goal is to design imaging schemes can be used out of laboratory. In paper, we introduce and test a pocket holographic slide allows digital holography microscopy performed without interferometer setup. Instead, commercial off-the-shelf plastic chip engineered functionalized with aim. The microfluidic endowed micro-optics, is, diffraction grating...
Abstract Imaging flow cytometry (IFC) combines and fluorescence microscopy to enable high-throughput, multiparametric single-cell analysis with rich spatial details. However, current IFC techniques remain limited in their ability reveal subcellular information a high 3D resolution, throughput, sensitivity, instrumental simplicity. In this study, we introduce light-field cytometer (LFC), an system capable of high-content, single-shot, multi-color acquisition up 5,750 cells per second...
In the current trend of miniaturization and simplification imaging flow cytometry, Lab-on-a-Chip (LoC) microfluidic devices represent an innovative cost-effective solution. this framework, we propose for first time a novel platform based on compactness holographic microscope slide (HMS) in combination with new computational features space-time digital holography (STDH) that uses 1D linear sensor array (LSA) instead 2D CCD or CMOS cameras to respond real diagnostic needs. LoC platform,...
Holographic microscopes are emerging as suitable tools for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through small compact devices. In-line holographic can be realized at contained costs, trading off complexity the phase retrieval process being limited to sparse samples. Here we present a 3D printed, cost effective field portable off-axis microscope based on concept of microfluidic slide. Our scheme removes from...
Antiviral signaling, immune response and cell metabolism are dysregulated by SARS-CoV-2, the causative agent of COVID-19. Here, we show that SARS-CoV-2 accessory proteins ORF3a, ORF9b, ORF9c ORF10 induce a significant mitochondrial metabolic reprogramming in A549 lung epithelial cells. While induced largely overlapping transcriptomes, ORF3a distinct transcriptome, including downregulation numerous genes with critical roles function morphology. On other hand, all four ORFs altered dynamics...
Imaging flow cytometry (IFC) combines conventional with optical microscopy, allowing for high-throughput, multi-parameter screening of single-cell specimens morphological and spatial information.
In this paper, we introduce a single-step self-assembling process aimed at forming two-dimensional (2-D) array microstructures made from nanocomposite polymer layer in which are dispersed CdSe-CdS nanocrystals. The novelty of the reported here is that it operates simultaneously as two-fold where liquid matrix self-shaped by electrohydrodynamic pressure 2-D microstructures, while same time, nanocrystals self-assembled dielectrophoretic forces. proposed approach could inspire future smart...
We designed, constructed and tested a holographic surface plasmon resonance (HoloSPR) objective-based microscope for simultaneous amplitude-contrast phase-contrast imaging (SPRi). SPRi is widely spread tool label-free detection of changes in refractive index concentration, as well mapping thin films. Currently, most the SPR sensors rely on amplitude or phase light. Despite high sensitivities achieved so far, each technique alone has limited range with optimal sensitivity. Here we use...
The dynamics and stability of thin liquid films have fascinated scientists over many decades. Thin film flows are central to numerous areas engineering, geophysics, biophysics occur a wide range lengths, velocities, property scales. In spite significant developments in this area, we still lack appropriate quantitative experimental tools with the spatial temporal resolution necessary for comprehensive study evolution. We propose tackling problem holographic technique that combines phase...
Fluorescence microscopy is one of the most indispensable and informative driving forces for biological research, but extent observable phenomena essentially determined by content quality acquired images. To address different noise sources that can degrade these images, we introduce an algorithm multiscale image restoration through optimally sparse representation (MIRO). MIRO a deterministic framework models acquisition process uses pixelwise correction to improve quality. Our study...
Fourier Ptychography probes the sample from different directions to achieve label-free quantitative phase imaging with a large space-bandwidth product. However, special attention has be paid in calibration of optical setup assure accurate knowledge geometrical parameters involved image reconstruction. Any slight misalignment can provoke incorrect synthesis observables and, turn, severe errors resulting high-resolution image. Here, we present new processing pipeline that automatically removes...
Electrohydrodynamic jetting is emerging as a successful technique for printing inks with resolutions well beyond those offered by conventional inkjet printers. However, the variety of printable still limited to relatively low viscosities (typically <20 mPa s) due nozzle clogging problems. Here, we show possibility ordered microdots high viscous such poly(lactic-co-glycolic acid) (PLGA) exploiting spontaneous breakup thin fiber generated through nozzle-free pyro-electrospinning. The PLGA...
In recent years, the use of probiotics in food and health has increased so much that usually market offers several functional fermented or nutraceuticals containing probiotics, often also associated to prebiotics. Both industry pharmaceutics, it is very important development methodologies quickly allow a precise overview about microbial population present specific biological matrix, monitor over time any changes may undergo. this paper, we propose biospeckle decorrelation as tool for fast...
The study of cell adhesion contacts is pivotal to understand mechanics and interaction at substrates or chemical physical stimuli. We designed built a HoloTIR microscope for label-free quantitative phase imaging total internal reflection. Here we show the first time that good choice focal cell/substrate as its sensitivity enhanced in comparison with standard TIR microscopy. Finally, simplicity implementation relative low cost, due requirement less optical components, make reasonable...
We report an optofluidic microscope that exploits multi-focal excitation using the fluidic motion of specimens for super-resolution, live-cell imaging.
Super-resolution fluorescence microscopy has revolutionized cell biology over the past decade, enabling visualization of subcellular complexity with unparalleled clarity and detail. However, rapid development image-scanning-based super-resolution systems still restrains convenient access to commonly used instruments such as epi-fluorescence microscopes. Here, we present multifocal scanning (MSM) for imaging simultaneous multicolor acquisition minimal instrumental complexity. MSM implements a...
The surface of a c− cut ferroelectric crystal at room temperature is characterized by the so‐called screening charges, able to compensate charge due spontaneous polarization. Recently, these charges inspired investigation interaction affinity live cells with lithium niobate and tantalate crystals. However, different knowledge gaps still remain that prevent reasonable application materials for biological applications. Here, label‐free holographic total internal reflection microscopy shown;...
Abstract Fluorescence microscopy has undergone rapid advancements, offering unprecedented visualization of biological events and shedding light on the intricate mechanisms governing living organisms. However, exploration dynamics still poses a significant challenge due to limitations current digital camera architectures inherent compromise between imaging speed other capabilities. Here, we introduce sHAPR, high-speed acquisition technique that leverages operating principles sCMOS cameras...
Fluorescence live-cell imaging allows for continuous interrogation of cellular behaviors, and the recent development portable platforms has rapidly transformed conventional schemes with high adaptability, cost-effective functionalities easy accessibility to cell-based assays. However, broader applications remain restrictive due compatibility cell culture workflow biochemical sensors, up-right physiological imaging, or parallelization data acquisition. Here, we introduce miniaturized...
Red blood cells on the surface of a lithium niobate crystal can be used as optical lenses for direct writing laser-induced refractive index changes. The process by such photomask made biological is due to photorefractive effect. Wavefront analysis digital holographic microscope performed deep and accurate evaluation local Different focusing properties imprinted depending which type RBC employed, discocytes or spherical-like RBCs. possibility fix into solid material fingerprint RBCs will have...
The development of tools for rapid food quality inspection is a highly pursued goal. These could be valuable devices to used by producers in factories or the customers themselves specific installations at marketplace. Here we show how speckle patterns coherent imaging systems can employed as indicators presence bacteria colonies contaminating water. Speckle decorrelation induced self-propelling movement these organisms when they interact with light. Hence, their detected using simple setup...
In global healthcare and point-of-care diagnostics there is an increasing request of medical equipment with devices able to provide fast reliable testing for clinical diagnosis. developing countries that lack adequate facilities, this need even more urgent. Lab-on-a-Chip have undergone a great growth during the last decade, supported by optical imaging techniques refined. Here we present recent progresses in tools based on holographic microscopy can be very useful when applied into...