A5046909586- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Genomics and Phylogenetic Studies
- Hepatitis Viruses Studies and Epidemiology
- Virus-based gene therapy research
- RNA modifications and cancer
- interferon and immune responses
- Viral Infections and Immunology Research
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Streptococcal Infections and Treatments
- Liver Disease and Transplantation
- Gut microbiota and health
- Probiotics and Fermented Foods
- Animal Virus Infections Studies
- Enzyme Structure and Function
- Advanced Biosensing Techniques and Applications
- Helicobacter pylori-related gastroenterology studies
- Nanoplatforms for cancer theranostics
- Bacterial biofilms and quorum sensing
- Gastrointestinal disorders and treatments
University of Córdoba
2022-2024
Instituto Maimónides de Investigación Biomédica de Córdoba
2022-2024
Biomedical Research Institute
2022-2023
Département Génétique Animale
2022-2023
Université Paris Cité
2019-2021
Institut Pasteur
2019-2021
Centre National de la Recherche Scientifique
2020-2021
Sorbonne Paris Cité
2019
Université Paris-Saclay
2016-2018
Microbiologie de l’alimentation au service de la santé
2015-2018
Wastewater pathogen monitoring is useful for surveillance of enteric pathogens. Information about the presence Paslahepevirus balayani (HEV) and emergent Rocahepevirus ratti (RHEV) in untreated water their correlation with clinical cases scarce. Aim To longitudinally monitor HEV RHEV wastewater to evaluate possible human cases.This study was carried out city Cordoba (southern Spain) from March 2021 2023. occurrence were evaluated by PCR three sample types: i) sera patients acute hepatitis...
SARS-CoV-2, the causative agent of present COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome, and some have been implicated facilitating infection pathogenesis through their interaction with cellular components. Among these proteins, protein ORF7a ORF7b functions are poorly understood. In this study, A549 cells were transduced to express ORF7b, respectively, explore more depth role each pathological manifestation leading COVID-19. Bioinformatic analysis integration...
Antiviral signaling, immune response and cell metabolism are dysregulated by SARS-CoV-2, the causative agent of COVID-19. Here, we show that SARS-CoV-2 accessory proteins ORF3a, ORF9b, ORF9c ORF10 induce a significant mitochondrial metabolic reprogramming in A549 lung epithelial cells. While induced largely overlapping transcriptomes, ORF3a distinct transcriptome, including downregulation numerous genes with critical roles function morphology. On other hand, all four ORFs altered dynamics...
Clostridium difficile is the leading cause of antibiotic-associated diarrhea in adults. During infection, C. must detect host environment and induce an appropriate survival strategy. Signal transduction networks involving serine/threonine kinases (STKs) play key roles adaptation, as they regulate numerous physiological processes. PrkC STK with two PASTA domains. We showed that membrane associated found at septum.
SARS-CoV-2, the cause of COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome. Their roles during infection are still not completely understood. In this study, transcriptomics analysis revealed that both WNT5A and IL11 were significantly up-regulated A549 cells expressing individual ORF6, ORF8, ORF9b or ORF9c from SARS-CoV-2 (Wuhan-Hu-1 isolate). is a member IL6 family cytokines. signaling-related genes also differentially expressed. Bioinformatics disclosed involved...
Rat hepatitis E virus (ratHEV; species Rocahepevirus ratti) is considered a newly emerging cause of acute zoonotic origin. ratHEV infection people living with HIV (PLWH) might portend worse, as (HEV; Paslahepevirus balayani), and consequently this group may constitute high-risk population. We aimed to evaluate the prevalence by measuring viral RNA specific IgG antibodies in large Spanish cohort PLWH. Multicenter study conducted Spain evaluating PLWHIV included AIDS Research Network (CoRIS)....
Rat hepatitis E virus (ratHEV) is an emerging cause of acute zoonotic origin. Since seroprevalence studies are scarce, at-risk groups almost unknown. Because blood-borne infections frequently occur in people with drug use, who particularly vulnerable to infection due lack housing and homelessness, this population constitutes a priority which ratHEV should be evaluated. Therefore, the aim study was evaluate RNA detection rate users as potential population. We designed retrospective involving...
YabA negatively regulates initiation of DNA replication in low-GC Gram-positive bacteria. The protein exerts its control through interactions with the initiator DnaA and sliding clamp DnaN. Here, we combined X-ray crystallography, scattering (SAXS), modeling biophysical approaches, vivo experimental data to gain insight into function. crystal structure N-terminal domain (NTD) solved at 2.7 Å resolution reveals an extended α-helix that contributes intermolecular four-helix bundle. Homology...
An optimized workflow for phosphopeptide enrichment has been developed in Clostridioides difficile revealing extensive protein phosphorylation.Using kinase or phosphatase mutant strains and quantitative proteomics, 41 114 downstream targets of the kinases PrkC CD2148, respectively, were identified C. difficile.We confirmed phosphorylation Spo0A vitro FtsK vivo. Highlights• protocol difficile.• About 117 events under control PASTA kinase, PrkC.• 27 PrkC-dependent more phosphorylated a...
Cell growth and division require a balance between synthesis hydrolysis of the peptidoglycan (PG). Inhibition PG or uncontrolled can be lethal for cells, making it imperative to control hydrolase (PGH) activity. The activity several key enzymes along biosynthetic pathway is controlled by Hanks-type serine/threonine kinases (STKs). In Gram-positive bacteria, inactivation genes encoding STKs associated with range phenotypes, including cell defects changes in wall metabolism, but only few...
Bacillus subtilis cells can adopt different life-styles in response to various environmental cues, including planktonic during vegetative growth, sessile biofilm formation and sporulation. While switching life-styles, bacteria must coordinate the progression of their cell cycle with physiological status. Our current understanding regulatory pathways controlling decision-making processes triggering developmental switches highlights a key role protein phosphorylation. The mechanisms that...
Abstract Cell growth and division require a balance between synthesis hydrolysis of the peptidoglycan (PG). Inhibition PG or uncontrolled can be lethal for cells, making it imperative to control hydrolase (PGH) activity. The serine/threonine kinases (STKs) Hanks family cell envelope homeostasis, but only few kinase substrates associated molecular mechanisms have been identified. In this work, we identified CwlA as first STK-PrkC substrate in human pathogen Clostridiodes difficile showed that...
From the early days of COVID-19 pandemic, an excessive release proinflammatory cytokines, such as IL6, was detected in serum from patients. As a consequence, several anti-inflammatory drugs, Dexamethasone (a strong corticoid), were used to counteract cytokine storm occurring during severe disease. By contrast, pro-inflammatory interleukin 11 (IL11), member IL6 family, respiratory tissues infected patients and experimental epithelial cellular models. In this work, human A549 lung cells...
ABSTRACT Antiviral signaling, immune response and cell metabolism in human body are dysregulated by SARS-CoV-2, the causative agent of COVID-19. Here, we show that SARS-CoV-2 accessory proteins ORF3a, ORF9b, ORF9c ORF10 induce a significant mitochondrial metabolic reprogramming A549 lung epithelial cells. While all four ORFs caused fragmentation altered function, only ORF3a induced marked structural alteration cristae. largely overlapping transcriptomes. In contrast, distinct transcriptome,...
WhiA is a conserved DNA-binding protein that influences cell division in many Gram-positive bacteria and, B. subtilis, also chromosome segregation. How works Bacillus subtilis unknown. Here, we tested three hypothetical mechanisms using metabolomics, fatty acid analysis, and confirmation capture experiments. This revealed does not influence segregation by modulating either central carbon metabolism or composition. However, the inactivation of reduces short-range interactions. These findings...
Abstract SARS-CoV-2, the causative agent of present COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome, and some have been implicated facilitating infection pathogenesis through their interaction with cellular components. Among these proteins, protein ORF7a ORF7b functions are poorly understood. In this study, A549 cells were transduced to express ORF7b, respectively, explore more depth role each pathological manifestation leading COVID-19. Bioinformatic analysis...
Summary SARS-CoV-2, the cause of COVID19 pandemic, possesses eleven accessory proteins encoded in its genome. Their roles during infection are still not completely understood. Transcriptomic analysis revealed that both WNT5A and IL11 were significantly up-regulated A549 cells expressing individual ORF6, ORF8, ORF9b or ORF9c from SARS-CoV-2 (Wuhan-Hu-1 isolate). signaling-related genes also differentially expressed. Bioinformatics disclosed involved pulmonary fibrosis idiopathic disease....
ABSTRACT The conserved WhiA protein family is present in most Gram-positive bacteria and plays a role cell division. contains DNA-binding motive has been identified as transcription factor actinomycetes. In Bacillus subtilis , the absence of influences division chromosome segregation, however, it still unclear how these processes, but does not seem to function this organism. To further investigate B. we performed yeast two-hybrid screen find interaction partners, Hi-C experiment reveal...