A5071536356- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Immune Cell Function and Interaction
- interferon and immune responses
- RNA regulation and disease
- COVID-19 Clinical Research Studies
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Mosquito-borne diseases and control
- RNA modifications and cancer
- Vector-Borne Animal Diseases
- Renal Transplantation Outcomes and Treatments
- Polyomavirus and related diseases
- Neurological Complications and Syndromes
- Mitochondrial Function and Pathology
- HIV Research and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- PI3K/AKT/mTOR signaling in cancer
Hospital Universitario 12 De Octubre
2021-2025
Instituto de Salud Carlos III
2022-2025
Centro de Investigación Biomédica en Red
2021-2025
Research Institute Hospital 12 de Octubre
2023-2024
Centre for Biomedical Network Research on Rare Diseases
2023-2024
Centro de Investigaciones Biológicas Margarita Salas
2021-2023
Hospital de Poniente
2023
Consejo Superior de Investigaciones Científicas
2010-2022
Hospital Universitario Fundación Jiménez Díaz
2020-2022
St. James's Hospital
2020
We characterized clinical isolates of Campylobacter using whole-genome sequencing (WGS) for detection virulence genes, antimicrobial resistance markers and phylogenetic analysis in order to increase the knowledge on molecular epidemiology Ireland, where there are significant gaps due widespread use culture independent methods diagnosis campylobacteriosis.WGS was applied 122 human collected over a 10-years period, from diarrhoeal stool samples submitted routine enteric screening.Genes...
SARS-CoV-2, the causative agent of present COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome, and some have been implicated facilitating infection pathogenesis through their interaction with cellular components. Among these proteins, protein ORF7a ORF7b functions are poorly understood. In this study, A549 cells were transduced to express ORF7b, respectively, explore more depth role each pathological manifestation leading COVID-19. Bioinformatic analysis integration...
ABSTRACT Background Kidney transplant (KT) recipients at intermediate risk for cytomegalovirus (CMV) infection constitute a potential target individualized prevention strategies informed by the CMV‐specific cell‐mediated immunity (CMV‐CMI). The optimal method functional assessment of CMV‐CMI in this group remains unclear. Methods We included 74 CMV‐seropositive KT that did not receive T‐cell‐depleting induction and were managed preemptive therapy. was monitored baseline months 1, 3, 6, 12...
ABSTRACT The impact of human cytomegalovirus (HCMV) infection on the mid‐ and long‐term balance between pro‐inflammatory anti‐inflammatory cytokines among kidney transplant recipients (KTRs) remains unclear. We measured plasma levels 12 Th1/Th2‐type (granulocyte‐macrophage colony‐stimulating factor, interferon‐γ, interleukin [IL]‐1β, IL‐2, IL‐4, IL‐5, IL‐6, IL‐10, IL‐12p70, IL‐13, IL‐18 tumor necrosis factor‐α) in a cohort 290 KTRs at four time points through month after transplantation....
Antiviral signaling, immune response and cell metabolism are dysregulated by SARS-CoV-2, the causative agent of COVID-19. Here, we show that SARS-CoV-2 accessory proteins ORF3a, ORF9b, ORF9c ORF10 induce a significant mitochondrial metabolic reprogramming in A549 lung epithelial cells. While induced largely overlapping transcriptomes, ORF3a distinct transcriptome, including downregulation numerous genes with critical roles function morphology. On other hand, all four ORFs altered dynamics...
ABSTRACT Virus infections can result in a variety of cellular injuries, and these often involve the permeabilization host membranes by viral proteins viroporin family. Prototypical 2B is responsible for alterations cell membrane permeability that take place enterovirus-infected cells. protein be localized at endoplasmic reticulum (ER) Golgi complex, inducing remodeling blockade glycoprotein trafficking. These findings suggest has potential to integrate into ER membrane, but specific...
Background and purpose Netrin‐1, an axon guidance protein, reduces serum levels of pro‐inflammatory mediators stabilizes the blood−brain barrier limiting entrance immune cells into central nervous system. The aim was to investigate its presence in experimental autoimmune encephalomyelitis ( EAE ) model multiple sclerosis MS patients with without clinical activity. Methods Netrin‐1 were evaluated mouse tissues. Afterwards, netrin‐1 cross‐sectionally quantified 90 different phenotypes 30...
SARS-CoV-2, the cause of COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome. Their roles during infection are still not completely understood. In this study, transcriptomics analysis revealed that both WNT5A and IL11 were significantly up-regulated A549 cells expressing individual ORF6, ORF8, ORF9b or ORF9c from SARS-CoV-2 (Wuhan-Hu-1 isolate). is a member IL6 family cytokines. signaling-related genes also differentially expressed. Bioinformatics disclosed involved...
Three different matrix (M) proteins termed M1, M2 and M3 have been described in cells infected with vesicular stomatitis virus (VSV). Individual expression of VSV M induces an evident cytopathic effect including cell rounding detachment, addition to a partial inhibition cellular protein synthesis, likely mediated by indirect mechanism. Analogous viroporins, M1 promotes the budding new particles; however, this process does not produce increase plasma membrane permeability. In contrast neither...
Members of the Mycobacterium abscessus complex (MABC) are multidrug-resistant nontuberculous mycobacteria and cause opportunistic pulmonary infections in individuals with cystic fibrosis (CF). In this study, genomic analysis MABC isolates was performed to gain greater insights into epidemiology circulating strains Ireland. Whole-genome sequencing (WGS) on 70 that had been referred Irish Mycobacteria Reference Laboratory between 2006 2017 across nine health care centers.
Objectives. To evaluate whether the use of platelet immunohistochemistry (IHC) markers improves sensitivity histological methods to detect microthrombosis in SLE nephritis and aPLs analyse clinicopathological correlations this setting. Methods. Kidney biopsy specimens from 65 patients with SLE, including 36 positive aPLs, were studied by IHC using antibodies against glycoproteins CD41 CD61. Clinical data at time kidney during a mean follow-up 7.5 years after recorded analysed regard or data....
Poliovirus RNA utilizes eIF2 for the initiation of translation in cell free systems. Remarkably, we now describe that poliovirus takes place at late times infection when is inactivated by phosphorylation. By contrast, directed blocked earlier times. Thus, exhibits a dual mechanism protein synthesis as regards to requirement eIF2. Analysis individual non-structural proteins indicates presence 2A(pro) alone sufficient provide independence IRES-driven translation. This effect not observed with...
Previous work by several laboratories has established that translation of picornavirus RNA requires active eIF2α for in cell free systems or after transfection culture cells. Strikingly, we have found encephalomyocarditis virus protein synthesis at late infection times is resistant to inhibitors induce the phosphorylation whereas early during blocked upon inactivation induced arsenite. The presence this compound first hour leads a delay appearance virus-infected Depletion also provokes...
Translation directed by several picornavirus IRES elements can usually take place after cleavage of eIF4G proteases 2A(pro) or L(pro). The hepatitis A virus (HAV) is thought to be an exception this rule because it requires intact eIF4F complex for translation. In line with previous results we report that poliovirus (PV) strongly blocks protein synthesis HAV IRES. However, in contrast findings now demonstrate foot-and-mouth disease (FMDV) L(pro) stimulates IRES-driven Thus, the first...
Risk stratification for cytomegalovirus (CMV) infection after kidney transplantation (KT) remains to be determined. Since endosomal toll-like receptors (TLRs) are involved in viral sensing, we investigated the impact of common single-nucleotide polymorphisms (SNPs) located within TLR3 and TLR9 genes on occurrence overall high-level (≥1,000 IU/ml) CMV a cohort 197 KT recipients. Homozygous carriers minor allele (rs3775291) had higher infection-free survival compared with reference (60.0% TT...
Abstract The best method for monitoring cytomegalovirus (CMV)‐specific cell‐mediated immunity (CMV‐CMI) among high‐risk kidney transplant (KT) recipients remains uncertain. We assessed CMV‐CMI by intracellular cytokine staining (ICS) flow cytometry and a commercial interferon (IFN)‐γ release assay (QuantiFERON®‐CMV [QTF‐CMV]) at posttransplant months 3, 4, 5 in 53 CMV‐seropositive KT that had received induction therapy with antithymocyte globulin (ATG) 3‐month course of valganciclovir...