A5034427288- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Polyomavirus and related diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Renal Transplantation Outcomes and Treatments
- Animal Virus Infections Studies
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 and COVID-19 Research
- Microscopic Colitis
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- Urinary Tract Infections Management
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Tuberculosis Research and Epidemiology
- Neurological Complications and Syndromes
- Parvovirus B19 Infection Studies
- Mosquito-borne diseases and control
- Vitamin D Research Studies
- Toxoplasma gondii Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HIV Research and Treatment
- Mycobacterium research and diagnosis
Hospital Universitario 12 De Octubre
2016-2025
Instituto de Salud Carlos III
2018-2025
Centro de Investigación Biomédica en Red
2022-2025
Research Institute Hospital 12 de Octubre
2016-2024
Hospital de Poniente
2023
Red Espanola de Investigacion en Patologia Infecciosa
2018
Universidad Complutense de Madrid
2015
The indication for antimicrobial treatment of asymptomatic bacteriuria (AB) after kidney transplantation (KT) remains controversial. Between January 2011 and December 2013, 112 KT recipients that developed one episode or more AB beyond the second month were included in this open-label trial. Participants randomized (1:1 ratio) to group (systematic therapy all episodes occurring ≤24 mo [53 patients]) control (no [59 patients]). Systematic screening was performed similarly both groups. primary...
ABSTRACT Background Kidney transplant (KT) recipients at intermediate risk for cytomegalovirus (CMV) infection constitute a potential target individualized prevention strategies informed by the CMV‐specific cell‐mediated immunity (CMV‐CMI). The optimal method functional assessment of CMV‐CMI in this group remains unclear. Methods We included 74 CMV‐seropositive KT that did not receive T‐cell‐depleting induction and were managed preemptive therapy. was monitored baseline months 1, 3, 6, 12...
ABSTRACT The impact of human cytomegalovirus (HCMV) infection on the mid‐ and long‐term balance between pro‐inflammatory anti‐inflammatory cytokines among kidney transplant recipients (KTRs) remains unclear. We measured plasma levels 12 Th1/Th2‐type (granulocyte‐macrophage colony‐stimulating factor, interferon‐γ, interleukin [IL]‐1β, IL‐2, IL‐4, IL‐5, IL‐6, IL‐10, IL‐12p70, IL‐13, IL‐18 tumor necrosis factor‐α) in a cohort 290 KTRs at four time points through month after transplantation....
The replication kinetics of nonpathogenic anelloviruses belonging to the Alphatorquevirus genus (such as torque teno virus) might reflect overall state posttransplant immunosuppression. We analyzed 221 kidney transplant (KT) recipients in whom plasma alphatorquevirus DNA load was quantified by real-time polymerase chain reaction at baseline and regularly through first 12 months. Study outcomes included infection a composite opportunistic and/or de novo malignancy (immunosuppression-related...
Antibiotic resistance is an emerging phenomenon in kidney transplantation (KT).We compared species distribution and antimicrobial susceptibility patterns 1052 isolates from urine cultures obtained 2 different cohorts of transplant recipients a single center (Cohort A: 189 patients undergoing KT between January 2002 December 2004 [336 isolates]; Cohort B: 115 2011 2013 [716 isolates]).Asymptomatic bacteriuria accounted for most the (86.9% A 92.3% B). Klebsiella pneumoniae (9.5% vs. 15.6%),...
Monitoring for cytomegalovirus (CMV)-specific cell-mediated immunity (CMV-CMI) may be useful individualizing valganciclovir (VGCV) prophylaxis after kidney transplantation (KT). We performed a commercial ELISA-based interferon (IFN)-γ release assay (QTF-CMV) from posttransplant months 2-5 (362 points) in 120 CMV-seropositive KT recipients that received antithymocyte globulin as induction therapy and VGCV (median of 92 days). Forty-seven patients (39.3%) had CMV infection discontinuation...
Severe acute respiratory syndrome coronavirus 2-specific cell-mediated immunity (SARS-CoV-2-CMI) elicited by mRNA-based vaccines in solid organ transplant (SOT) recipients and its correlation with antibody responses remain poorly characterized.We included 44 (28 kidney, 14 liver, 2 double organ) who received the full series of mRNA-1273 vaccine. SARS-CoV-2-CMI was evaluated at baseline, before second dose, wk after completion vaccination an ELISpot-based interferon-γ FluoroSpot assay using...
The increased risk of tuberculosis (TB) reactivation in solid organ transplant recipients supports the recommendation screening for latent infection (LTBI). Adherence to available tests has not been studied kidney (KT) population. We aimed assess compliance within ATALANTA-DOS population study. an intervention bundle at preventing KT recipients. compared LTBI rates between pre-intervention (February 2016 - September 2017) and 2018 2019) cohorts evaluated adherence interferon-gamma release...
The objective of the present study was to evaluate value PCR cycle threshold (CT ) for predicting recurrence/severity infection compared that toxin detection plus clinical variables. First episodes Clostridium difficile (CDI) diagnosed during 2015 at our institution were included. Samples tested glutamate dehydrogenase (GDH) and A/B by use a single enzyme immunoassay (EIA). Xpert C. assay performed on GDH-positive samples. Medical data reviewed investigators blinded diagnostic results...
The magnitude and kinetics of severe acute respiratory syndrome coronavirus 2-specific cell-mediated immunity (SARS-CoV-2-CMI) in kidney transplant (KT) recipients remain largely unknown.We enumerated SARS-CoV-2-specific interferon-γ-producing CD69+ CD4+ CD8+ T cells at months 4 6 from the diagnosis disease 2019 (COVID-19) 21 KT by intracellular cytokine staining. Overlapping peptides encompassing SARS-CoV-2 spike (S) glycoprotein N-terminal 1- to 643-amino acid sequence membrane protein...
Whether immunosuppression impairs severe acute respiratory syndrome coronavirus 2-specific T cell-mediated immunity (SARS-CoV-2-CMI) after liver transplantation (LT) remains unknown. We included 31 LT recipients in whom SARS-CoV-2-CMI was assessed by intracellular cytokine staining (ICS) and interferon (IFN)-γ FluoroSpot assay a median of 103 days from COVID-19 diagnosis. Serum SARS-CoV-2 IgG antibodies were measured ELISA. A control group nontransplant immunocompetent patients matched (1:1...
Risk stratification for cytomegalovirus (CMV) infection after kidney transplantation (KT) remains to be determined. Since endosomal toll-like receptors (TLRs) are involved in viral sensing, we investigated the impact of common single-nucleotide polymorphisms (SNPs) located within TLR3 and TLR9 genes on occurrence overall high-level (≥1,000 IU/ml) CMV a cohort 197 KT recipients. Homozygous carriers minor allele (rs3775291) had higher infection-free survival compared with reference (60.0% TT...
Abstract The best method for monitoring cytomegalovirus (CMV)‐specific cell‐mediated immunity (CMV‐CMI) among high‐risk kidney transplant (KT) recipients remains uncertain. We assessed CMV‐CMI by intracellular cytokine staining (ICS) flow cytometry and a commercial interferon (IFN)‐γ release assay (QuantiFERON®‐CMV [QTF‐CMV]) at posttransplant months 3, 4, 5 in 53 CMV‐seropositive KT that had received induction therapy with antithymocyte globulin (ATG) 3‐month course of valganciclovir...
Abstract Background The transmembrane glycoprotein CD 30 contributes to regulate the balance between Th 1 and 2 responses. Previous studies have reported conflicting results on utility of its soluble form ( sCD 30) predict post‐transplant infection. Methods Serum was measured by a commercial ELISA assay at baseline months 1, 3, 6 in 100 kidney transplant KT ) recipients (279 monitoring points). impact levels incidence overall, bacterial opportunistic infection during first 12 after...
Abstract Background Recent studies have reported an increased susceptibility to infection among vitamin D‐deficient kidney transplant ( KT ) recipients, although methodological concerns remain. Methods Serum 25‐hydroxyvitamin D (25( OH )D) levels were measured in 246 recipients at post‐transplant months 1, 3, 6 and 12. Vitamin status was analysed terms of deficiency (Endocrine Society [<20 ng/mL] Institute Medicine [IoM, <12 criteria) as a continuous variable. Cox models for overall,...
The immunogenicity elicited by the Omicron BA.4/BA.5-adapted bivalent booster vaccine after solid organ transplantation (SOT) has not been characterized.
The QuantiFERON-Monitor assay (QTF-Monitor) is intended to assess innate and adaptive immune responses by quantifying interferon (IFN)-γ release upon whole blood stimulation with a TLR7/8 agonist an anti-CD3 antibody. We performed the QTF-Monitor in 126 kidney transplant recipients (KTRs) at different points during first 6 post-transplant months. primary outcome was overall infection, whereas secondary outcomes included bacterial opportunistic infection