A5070279839- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Dental Research and COVID-19
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Prion Diseases and Protein Misfolding
- Glycosylation and Glycoproteins Research
- Cell death mechanisms and regulation
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Oral microbiology and periodontitis research
- Cystic Fibrosis Research Advances
- RNA Research and Splicing
- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- COVID-19 diagnosis using AI
- Insect symbiosis and bacterial influences
- Protein purification and stability
- Enzyme Structure and Function
- Vector-borne infectious diseases
- Supramolecular Self-Assembly in Materials
- Plant Stress Responses and Tolerance
- Ion Channels and Receptors
Universitat de València
2010-2025
Dentaid (Spain)
2022-2024
Universitat Autònoma de Barcelona
2017-2019
The aggregation propensity of each particular protein seems to be shaped by evolution according its natural abundance in the cell. production and downstream processing recombinant polypeptides implies attaining concentrations that are orders magnitude above their levels, often resulting aggregation; a phenomenon precludes marketing many globular proteins for biomedical or biotechnological applications. Therefore, there is huge interest methods aimed increase solubility limits. Here, we...
SARS-CoV-2 is continuously disseminating worldwide. The development of strategies to break transmission mandatory.To investigate the potential cetylpyridinium chloride (CPC) as a viral inhibitor.SARS-CoV-2 Virus Like-Particles (VLPs) were incubated with CPC, potent surfactant routinely included in mouthwash preparations.Concentrations 0.05% CPC (w/v) commonly used preparations are sufficient promote rupture VLP membranes.Including mouthwashes could be prophylactic strategy keep from spreading.
Biological membranes consist of a lipid bilayer studded with integral and peripheral membrane proteins. Most α-helical proteins require protein-conducting insertases known as translocons to assist in their insertion folding. While the sequence-dependent propensities for helix either translocate through translocon or insert into have been codified numerical hydrophobicity scales, corresponding propensity partition interface remains unrevealed. By engineering diagnostic glycosylation sites...
N-glycosylation is the most common and versatile protein modification. In eukaryotic cells, this modification catalyzed cotranslationally by enzyme oligosaccharyltransferase, which targets β-amide of asparagine in an Asn-Xaa-Ser/Thr consensus sequon (where Xaa any amino acid but proline) nascent proteins as they enter endoplasmic reticulum. Because glycosylation acceptor site on membrane occurs a compartment-specific manner, presence used to indicate topology. Moreover, sites can be added...
Abstract Integral membrane proteins are assembled into the ER via a continuous ribosome-translocon channel. The hydrophobicity and thickness of core bilayer leads to expectation that transmembrane (TM) segments minimize cost harbouring polar polypeptide backbones by adopting regular pattern hydrogen bonds form α-helices before integration. Co-translational folding nascent chains an α-helical conformation in ribosomal tunnel has been demonstrated previously, but features governing this not...
ABSTRACT Virus infections can result in a variety of cellular injuries, and these often involve the permeabilization host membranes by viral proteins viroporin family. Prototypical 2B is responsible for alterations cell membrane permeability that take place enterovirus-infected cells. protein be localized at endoplasmic reticulum (ER) Golgi complex, inducing remodeling blockade glycoprotein trafficking. These findings suggest has potential to integrate into ER membrane, but specific...
The long‐standing paradigm that all peroxisomal proteins are imported post‐translationally into pre‐existing peroxisomes has been challenged by the detection of membrane ( PMPs ) inside endoplasmic reticulum ER ). In mammals, mechanisms entry and exit completely unknown. We show human PMP PEX3 inserts co‐translationally mammalian via Sec61 translocon. Photocrosslinking fluorescence spectroscopy studies demonstrate N‐terminal transmembrane segment TMS ribosome‐bound is recognized signal...
Oxidatively modified forms of proteins accumulate during aging. Oxidized protein conformers might act as intermediates in the formation amyloids age-related disorders. However, it is not known whether this amyloidogenic conversion requires an extensive oxidative damage or can be promoted just by a discrete, localized post-translational modification certain residues. Here, we demonstrate that irreversible oxidation single free Cys suffices to severely perturb folding energy landscape stable...
The vast majority of membrane proteins are anchored to biological membranes through hydrophobic α-helices. Sequence analysis high-resolution protein structures show that ionizable amino acid residues present in transmembrane (TM) helices, often with a functional and/or structural role. Here, using as scaffold the TM domain model glycophorin A (GpA), we address consequences replacing specific by acids on helix insertion and packing, both detergent micelles membranes. Our findings demonstrate...
The oral cavity is particularly susceptible to viral infections that are self-recovering in most cases. However, complications may appear severe cases and/or immunocompromised subjects. Cetylpyridinium chloride (CPC)-containing mouthwashes able decrease the infectivity of SARS-CoV-2 virus by disrupting integrity envelope. Here, we show CPC, as active ingredient contained commercialized, exerts significant antiviral activity against enveloped viruses, such HSV-1, but not non-enveloped HPV....
Amyloids have been exploited to build amazing bioactive materials. In most cases, short synthetic peptides constitute the functional components of such The controlled assembly globular proteins into active amyloid nanofibrils is still challenging, because formation amyloids implies a conformational conversion towards β-sheet-rich structure, with concomitant loss native fold and inactivation protein. There is, however, remarkable exception this rule: yeast prions. They are singular able...
Mutations or cellular conditions that destabilize the native protein conformation promote population of partially unfolded conformations, which in many cases assemble into insoluble amyloid fibrils, a process associated with multiple human pathologies. Therefore, stabilization structures is seen as an efficient way to prevent misfolding and subsequent aggregation. This has been suggested be underlying reason why proteins living harsh environments, such extracellular space, have evolved...
Background The oral cavity is the site of entry and replication for many respiratory viruses. Furthermore, it source droplets aerosols that facilitate viral transmission. It thought appropriate hygiene alters infectivity might reduce spread viruses contribute to infection control. Materials methods Here, we analyzed antiviral activity cetylpyridinium chloride (CPC), chlorhexidine (CHX), three commercial CPC CHX-containing mouthwash preparations against Influenza A virus Respiratory syncytial...
A peptide corresponding to bacteriorhodopsin (bR) helix C, later named pHLIP, inserts across lipid bilayers as a monomeric α-helix at acidic pH, but is an unstructured surface-bound monomer neutral pH. As result of such pH-responsiveness, pHLIP targets tumors and has been used vehicle for imaging drug-delivery cargoes. To gain insights about the insertion bR C into biological membranes, we replaced two key aspartic residues that control topological transition from aqueous phase bilayer....
Abstract Biological membranes consist of a lipid bilayer studded with integral and peripheral membrane proteins. Most α-helical proteins require protein-conducting insertases known as translocons to assist in their insertion folding. While the sequence-dependent propensities for helix either translocate through translocon or insert into have been codified numerical hydrophobicity scales, corresponding propensity partition interface remains unraveled. By engineering diagnostic glycosylation...
The bacterial spirochete Borrelia burgdorferi is the causative agent of most commonly reported arthropod-borne illness in United States, Lyme disease. A family proteins containing von Willebrand Factor (VWFA) domains adjacent to a MoxR AAA+ ATPase have been found be highly conserved genus Borrelia. Previously, VWFA domain protein B. burgdorferi, BB0172, was determined an outer membrane capable binding integrin α3β1. In this study, characterization new protein, BB0173, evaluated order define...