Summary Borrelia burgdorferi , the aetiological agent of Lyme disease, utilizes multiple adhesins to interact with both arthropod vector and mammalian hosts it colonizes. One such adhesive molecule is a surface‐exposed fibronectin‐binding lipoprotein, designated BBK32. Previous characterization BBK32‐mediated fibronectin binding has been limited biochemical analyses due difficulty in mutagenizing infectious isolates B. . Here we report an alternative method inactivate bbk32 via allelic...

Disease risk maps are important tools that help ascertain the likelihood of exposure to specific infectious agents. Understanding how climate change may affect suitability habitats for ticks will improve accuracy tick-borne pathogen transmission in humans and domestic animal populations. Lyme disease (LD) is most prevalent arthropod borne US Europe. The bacterium Borrelia burgdorferi causes LD it transmitted other mammalian hosts through bite infected Ixodes ticks. transboundary region...

Summary Borrelia burgdorferi , the causative agent of Lyme disease, has a limited set genes to combat oxidative/nitrosative stress encountered in its tick vector or mammalian hosts. We inactivated gene encoding for superoxide dismutase A ( sodA bb0153 ), an enzyme mediating dismutation anions and examined vitro vivo phenotype mutant. There were no significant differences growth characteristics mutant compared with control strains. Microscopic analysis viability spirochaetes revealed greater...

ABSTRACT Carbon storage regulator A (CsrA) is an RNA binding protein that has been characterized in many bacterial species to play a central regulatory role by modulating several metabolic processes. We recently showed homolog of CsrA Borrelia burgdorferi (CsrA Bb , BB0184) was upregulated response propagation B. under mammalian host-specific conditions. In order further delineate the we generated deletion mutant designated ES10 linear plasmid 25-negative isolate strain B31 (ML23). The...

Background In the United States, Tick‐Borne Relapsing Fever (TBRF) in dogs is caused by spirochete bacteria Borrelia turicatae and hermsii , transmitted Ornithodoros spp . ticks. The hallmark diagnostic feature of this infection visualization numerous spirochetes during standard blood smear examination. Although course spirochetemia has not been fully characterized dogs, humans infected with TBRF episodes fever are intermittent. Objectives To describe providing additional case reports...

Borrelia burgdorferi, the causative agent of Lyme disease, undergoes rapid adaptive gene expression in response to environmental signals encountered during different stages its life cycle arthropod vector or mammalian host. Among all plasmid-encoded genes B. several linear plasmid 54 (lp54)-encoded open reading frames (ORFs) exhibit greatest differential host-specific temperature, pH, and other uncharacterized signals. These ORFs include members paralogous family (pgf 54), such as BBA64,...

Borrelia burgdorferi, the agent of Lyme disease, alters its gene expression in response to highly disparate environmental signals encountered hosts. Among relatively few regulators adaptive present borrelial genome is an open reading frame (ORF), BB0184, annotated as CsrA (carbon storage regulator A). CsrA, several bacterial species, has been characterized a small RNA binding protein that functions global affecting mRNA stability or levels translation multiple ORFs. Consistent with known...

ABSTRACT Borrelia burgdorferi , the agent of Lyme disease, undergoes rapid adaptive gene expression in response to signals unique its arthropod vector or vertebrate hosts. Among upregulated genes under host conditions is one five annotated homologs oligopeptide permease A (OppA5, BBA34). mutant lacking oppA5 was constructed an lp25-deficient isolate B. strain B31, and minimal regions infectivity were restored via a shuttle pBBE22 with without intact copy bba34 . Immunoblot analysis revealed...

Rickettsial infection in dog-associated ticks three rural communities of Yucatan, Mexico was investigated using qPCR and nested PCR assays. A total 319 dogs were studied samples collected. 170 infested with (frequency 53.4%). Overall, 1,380 representing seven species collected: Amblyomma mixtum, A. ovale, parvum, cf. oblongoguttatum, Ixodes affinis, Rhipicephalus microplus, R. sanguineus sensu lato. The most abundant s.l. a mean intensity 7.4 ticks/host. Dogs the Chan San Antonio Yaxcheku...

Ticks display a distinct type of host-seeking behaviour called questing. It has been proposed that the questing Ixodes scapularis explains geographic variation in Lyme disease (LD) risk eastern USA because northern population shown to quest more often than southern population. The height at which occurs is variable and this study aimed characterize for I. scapularis. were collected from state (i.e. Maryland Texas) bioassays conducted. We report nymphs Texas quested lower heights compared...

Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi infection. Upon infection, some B. genes are upregulated, including members of the microbial surface components recognizing adhesive matrix molecule (MSCRAMM) protein family, which facilitate adherence to extracellular host. Comparative genome analysis has revealed new family proteins containing von Willebrand factor A (vWFA) domain. In present study, we characterized expression and membrane association vWFA...

Ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. The prevalence this disease in veterinary medicine can vary depending on the diagnostic method used geographic location. One hundred fifty-two blood samples from six clinics two shelters Sinaloa State (Mexico) were analyzed study. All animals suspected having Canine Monocytic Ehrlichiosis (CME). methods ELISA (Snap4Dx, IDEXX) together with smear platelet count. From all dogs...

Borrelia burgdorferi, the causative agent of Lyme disease, alters its gene expression in response to environmental signals unique tick vector or vertebrate hosts. B. burgdorferi carries one superoxide dismutase (sodA) capable controlling intracellular levels. Previously, sodA was shown be essential for infection C3H/HeN model disease. We employed two-dimensional electrophoresis (2-DE) and immunoblot analysis with antibodies specific carbonylated proteins identify targets that were...

Determining which wildlife hosts are involved in the enzootic cycles of tick-borne diseases (TBD) enables enhanced surveillance and risk assessment potential transmission to humans domestic species. Currently, there is limited data indicate pathogens (TBP) can infect coyotes. Additionally, for white-tailed deer (WTD) south Texas available. The purpose this study was detect current infections common TBP coyotes WTD Texas, represents a transboundary region site animal migrations across...