A5102707770- Vector-borne infectious diseases
- Bacterial biofilms and quorum sensing
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Protein purification and stability
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Heat shock proteins research
- Yersinia bacterium, plague, ectoparasites research
- Clostridium difficile and Clostridium perfringens research
- Fungal Infections and Studies
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Toxin Mechanisms and Immunotoxins
- Bacillus and Francisella bacterial research
- Mosquito-borne diseases and control
- Metabolomics and Mass Spectrometry Studies
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
Texas Center for Infectious Disease
2010-2017
The University of Texas at San Antonio
2010-2017
United States Army Institute of Surgical Research
2016
Texas A&M University
2010
Universitat de València
2010
Johns Hopkins University
2003-2007
Public Health Laboratory
1969
Statistical Research (United States)
1961
Medical Research Council
1961
The RpoS transcription factor of Borrelia burgdorferi is a 'gatekeeper' because it activates genes required for spirochaetes to transition from tick vertebrate hosts. However, remains unknown how becomes repressed allow the back host vector. Here we show that putative carbohydrate-responsive regulatory protein, designated BadR (Borrelia adaptation Regulator), transcriptional repressor rpoS. levels are elevated in B. cultures grown under vitro conditions mimicking unfed-ticks and...
Biofilm development, specifically the fundamentally adaptive switch from acute to chronic infection phenotypes, requires global regulators and small non-coding regulatory RNAs (sRNAs). This work utilized RNA-sequencing (RNA-seq) detect sRNAs differentially expressed in Pseudomonas aeruginosa biofilm versus planktonic state.A computational algorithm was devised categorize into 5 types: intergenic, intragenic, 5'-UTR, 3'-UTR, antisense. Here we report a novel RsmY/RsmZ-type sRNA, termed RsmW,...
ABSTRACT Carbon storage regulator A (CsrA) is an RNA binding protein that has been characterized in many bacterial species to play a central regulatory role by modulating several metabolic processes. We recently showed homolog of CsrA Borrelia burgdorferi (CsrA Bb , BB0184) was upregulated response propagation B. under mammalian host-specific conditions. In order further delineate the we generated deletion mutant designated ES10 linear plasmid 25-negative isolate strain B31 (ML23). The...
The presence of specific proteins, including Ece1p, Hwp1p and Als3p, distinguishes the Candida albicans hyphal cell wall from that yeast-form cells. These proteins are thought to be important for ability C. cells adhere living non-living surfaces cell-to-cell adhesion necessary biofilm formation, also pivotal in mediating interactions with endothelial Using an vitro flow assay, we previously observed yeast bind greater numbers human microvascular than do or pseudohyphal This is consistent...
Borrelia burgdorferi, the agent of Lyme disease, is a spirochetal pathogen with limited metabolic capabilities that survives under highly disparate host-specific conditions. However, borrelial genome encodes several proteins mevalonate pathway (MP) utilizes acetyl-CoA as substrate leading to intermediate metabolites critical for biogenesis peptidoglycan and post-translational modifications proteins. In this study, we analyzed MP contributions acetate in modulation adaptive responses B....
Abstract Pseudomonas aeruginosa and Staphylococcus aureus mixed‐species biofilm infections are more resilient to biocide attacks compared their single‐species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen vivo investigate the interactions of P. S. biofilms. RNA sequencing ( ‐seq) was utilized identify entire genomic response, both open reading frames ORF s) small s sRNA s), each species. Using competitive indexes, transposon mutants...
ABSTRACT Borrelia burgdorferi , the agent of Lyme disease, undergoes rapid adaptive gene expression in response to signals unique its arthropod vector or vertebrate hosts. Among upregulated genes under host conditions is one five annotated homologs oligopeptide permease A (OppA5, BBA34). mutant lacking oppA5 was constructed an lp25-deficient isolate B. strain B31, and minimal regions infectivity were restored via a shuttle pBBE22 with without intact copy bba34 . Immunoblot analysis revealed...
Influenza vaccines prepared with a mineral oil adjuvant induce substantial and durable antibody response. However, containing in simple emulsion (water-in-oil emulsion) sometimes produce persistent local reactions (Medical Research Council, 1964). The development of redispersed ‘multiple’ which antigen is incorporated as an oil-in-water starting from original water-in-oil was described by Herbert (1965). Such multiple vaccine has lesser viscosity than emulsions might therefore response fewer...
Carbon storage regulator A of Borrelia burgdorferi (CsrABb) contributes to vertebrate host-specific adaptation by modulating activation the Rrp2-RpoN-RpoS pathway and is critical for infectivity. We hypothesized that functions CsrABb are dependent on environmental signals select residues. analyzed phenotype csrABb deletion site-specific mutants determine conserved pathogen-specific attributes CsrABb. Levels phosphate acetyltransferase (Pta) involved in conversion acetyl acetyl-coenzyme...
Borrelia burgdorferi, the agent of Lyme disease, responds to numerous host-derived signals alter adaptive capabilities during its enzootic cycle in an arthropod vector and mammalian host. Molecular mechanisms that enable B. burgdorferi detect, channel, respond these have become intense area study for developing strategies limit transmission/infection. Bioinformatic analysis borrelial genome revealed presence polyamine transport components (PotA, PotB, PotC, PotD), while homologs biosynthesis...
Infection with Pseudomonas aeruginosa leads to impairment of healing and many deaths in severe burn patients. The phenotypic diversity P. strains makes it difficult define a therapeutic strategy. Here we report the genome sequence highly virulent strain aeruginosa, VA-134, isolated from patient.
In 1957, freeze-dried B.C.G. vaccine, prepared in Britain by Glaxo Laboratories Ltd., and liquid Denmark the State Serum Institute, were compared for their ability to induce tuberculin sensitivity produce local vaccination lesions (Medical Research Council, 1958Council, , 1960).The trials made six areas Middlesex among 13-and 14-year-old schoolchildren, who divided according month of birth into two groups similar size.One group was vaccinated with Danish vaccine; 15 batches used, each within...