High-throughput single-cell analysis is a challenging task. Label-free tomographic phase microscopy an excellent candidate to perform this However, in-line tomography very difficult implement in practice because it requires complex set-up for rotating the sample and examining cell along several directions. We demonstrate that by exploiting random rolling of cells while they are flowing microfluidic channel, possible obtain phase-contrast tomography, if smart strategies wavefront adopted. In...

Abstract Quantitative phase imaging has gained popularity in bioimaging because it can avoid the need for cell staining, which, some cases, is difficult or impossible. However, as a result, quantitative does not provide labelling of various specific intracellular structures. Here we show novel computational segmentation method based on statistical inference that makes possible techniques to identify nucleus. We demonstrate approach with refractive index tomograms stain-free cells...

The perspective of using live cells as lenses could open new revolutionary and intriguing scenarios in the future biophotonics biomedical sciences for endoscopic vision, local laser treatments via optical fibres diagnostics. Here we show that a suspended red blood cell (RBC) behaves an adaptive liquid-lens at microscale, thus demonstrating its imaging capability tunable focal length. In fact, thanks to intrinsic elastic properties, RBC can swell up from disk volume 90 fl sphere reaching 150...

Sperm morphology is regarded as a significant prognostic factor for fertilization, abnormal sperm structure one of the most common factors in male infertility. Furthermore, obtaining accurate morphological information an important issue with strong implications zoo-technical industries, example to perform sorting species X from Y. A challenging step forward would be availability fast, high-throughput and label-free system measurement physical parameters visualization 3D shape such biological...

Microplastics (MPs) are a major environmental concern due to their possible impact on water pollution, wildlife, and the food chain. Reliable, rapid, high‐throughput screening of MPs from other components sample after sieving and/or digestion is still highly desirable goal avoid cumbersome visual analysis by expert users under optical microscope. Here, new approach presented that combines 3D coherent imaging with machine learning (ML) achieve accurate automatic detection in filtered samples...

Abstract Three dimensional (3D) morphometric analysis of flowing and not‐adherent cells is an important aspect for diagnostic purposes. However, diagnostics tools need to be quantitative, label‐free and, as much possible, accurate. Recently, a simple holographic approach, based on shape from silhouette algorithm, has been demonstrated accurate calculation biovolume displaying their 3D shapes. Such approach adopted in combination with optical tweezers successfully applied convex shape....

We report a reliable full-angle tomographic phase microscopy (FA-TPM) method for flowing quasi-spherical cells along microfluidic channels. This lies in completely passive optical system, i.e. mechanical scanning or multi-direction probing of the sample is avoided. It exploits engineered rolling while they are channel. Here we demonstrate significant progress with respect to state art in-flow TPM by showing general extension having almost spherical shapes suspension. In fact, adopted...

Abstract Circulating tumor cells (CTCs) are rare released from primary, metastatic, or recurrent tumors in the peripheral blood of cancer patients. CTCs isolation and their molecular characterization represent a new marker screening, diagnostic tool called “liquid biopsy” (LB). Compared to traditional tissue biopsy that is invasive does not reveal heterogeneity, LB noninvasive reflects “real‐time” dynamism drug sensitivity. In frame LB, paradigm based on single‐cell label‐free analysis...

An approach that uses an electro-optically tunable two dimensional phase grating to enhance the resolution in digital holographic microscopy is proposed. We show that, by means of a flexible hexagonal grating, it possible increase numerical aperture imaging system, thus improving spatial images dimensions. The augment optical system obtained recording spatially multiplexed holograms. tuneability allows one adjust intensity among holograms maximizing diffraction efficiency. Furthermore we...

In liquids realm, surface tension and capillarity are the key forces driving formation of shapes pervading nature. The steady dew drops appearing on plant leaves spider webs result from minimization overall energy [Zheng Y, et al. (2010) Nature 463:640-643]. Thanks to tension, interfaces such spontaneous structures exhibit extremely good spherical shape consequently worthy optical quality. Also nanofluidic instabilities generate a variety fascinating liquid silhouettes, but they however...

Today, fast and accurate diagnosis through portable cheap devices is in high demand for the general healthcare. Lab-on-chips (LoCs) have undergone a great growth this direction, supported by optical imaging techniques more refined. Here we present recent progresses developing tools based on coherent microscopy that can be very useful when applied into biomicrofluidics. In some cases, tweezers (OT) technique combined with digital holography (DH), thus offering possibility to manipulate,...

The 3D tracking of micro-objects, based on digital holography, is proposed through the analysis complex wavefront light scattered by micro-samples. Exploiting advantages off-axis full-field holographic interferometry, multiple objects achieved a direct at focal plane overcoming limitation conventional back interferometry in which only one object time can be tracked. Furthermore, method and demonstrated here step forward with respect to other tools. approach tested two experiments, first...

Water plastic pollution is a serious problem affecting sealife, marine habitats, and the food chain. Artificial intelligence-enabled coherent imaging has recently shown exciting advances in field of environmental monitoring, portable holographic microscopes are good candidates to map microparticles content waters. The "holographic fingerprint" due light diffraction rich information, fully encoded into complex wavefront scattered by sample. Hence, proper analysis wavefronts reconstructed from...

Although holography is topic that goes back to the 1950s, research in this field continues be very active worldwide. A continuous growth confirmed by publication of more than 2000 papers each year archival journal on different holographic issues. Here we describe shortly what appeared us most significant achievements reached 2013 imaging.

Imaging through turbid media is a challenging topic. A liquid considered when dispersed particles provoke strong light scattering, thus destroying the image formation by any standard optical system. Generally, colloidal solutions belong to class of since have dimensions ranging between 0.2 μm and 2 μm. However, in microfluidics, another relevant issue has be case flowing made multitude occluding objects, e.g. red blood cells (RBCs) veins. In such instead severe scattering processes...

The gold-standard methods for anemia diagnosis are complete blood counts and peripheral-smear observations. However, these do not allow a differential as that requires biochemical assays, which label-dependent techniques. On the other hand, recent studies focus on label-free quantitative phase imaging (QPI) of samples to investigate diseases by using video-based morphological methods. when sick cells very similar healthy ones in terms morphometric features, identification disease becomes...

Photothermal therapy (PTT) is an efficient method of inducing localized hyperthermia and can be achieved using gold nanoparticles as photothermal agents. However, there are many hurdles to get over before this safely reach the clinics, including nanoparticles' optimal shape accurate prediction cellular responses. Here, we describe synthesis nanorods nanoprisms with similar surface plasmon resonances in near-infrared (NIR) comparable conversion efficiencies characterize response NIR...

Interaction of nanoparticles (NPs) with cells is fundamental importance in biology and biomedical sciences. NPs can be taken up by cells, thus interacting their intracellular elements, modifying the life cycle pathways, possibly inducing death. Therefore, there a great interest understanding visualizing process cellular uptake itself or even secondary effects, for example, toxicity. Nowadays, no method reported yet which 3D imaging distribution achieved suspended flow-cytometry. Here we show...

Holographic tomography allows the 3D mapping of refractive index biological samples thanks to reconstruction methods based on knowledge illumination directions or rotation angles imaged sample. Recently, phase contrast tomographic flow cytometry by digital holography has been demonstrated reconstruct three-dimensional distribution single cells while they are flowing along microfluidic channels. In this system, direction is fixed sample's not deterministically known a priori but induced...

The label-free single cell analysis by machine and Deep Learning, in combination with digital holography transmission microscope configuration, is becoming a powerful framework exploited for phenotyping biological samples. Usually, quantitative phase images of cells are retrieved from the reconstructed complex diffraction patterns used as inputs deep neural network. However, retrieval process can be very time consuming prone to errors. Here we address classification using learning strategies...