A5068562700- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- Glioma Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Ancient Mediterranean Archaeology and History
- Classical Antiquity Studies
- COVID-19 Clinical Research Studies
- Lung Cancer Research Studies
- Protein Degradation and Inhibitors
- Liver Disease Diagnosis and Treatment
- Ancient Egypt and Archaeology
- Digital Holography and Microscopy
- Archaeology and Historical Studies
- Microfluidic and Bio-sensing Technologies
- Health Systems, Economic Evaluations, Quality of Life
- Cancer Treatment and Pharmacology
- Historical, Religious, and Philosophical Studies
- PARP inhibition in cancer therapy
- Cell Image Analysis Techniques
- Ubiquitin and proteasome pathways
- Erythrocyte Function and Pathophysiology
- Renaissance Literature and Culture
- Signaling Pathways in Disease
- Historical and Religious Studies of Rome
University of Naples Federico II
2016-2025
Ceinge Biotecnologie Avanzate (Italy)
2016-2025
Azienda Ospedaliera Ospedale Maggiore
2024-2025
Federico II University Hospital
2012-2024
Collaborative Group (United States)
2024
SDN Istituto di Ricerca Diagnostica e Nucleare
2009-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2009-2023
University of Padua
2013-2023
University of Siena
2023
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2023
Neuroblastoma is a malignant condition of the developing sympathetic nervous system that most commonly affects young children and often lethal. Its cause not known.
Background: Recently, loss-of-function variants in TLR7 were identified two families which COVID-19 segregates like an X-linked recessive disorder environmentally conditioned by SARS-CoV-2. We investigated whether the represent tip of iceberg a subset male patients. Methods: This is nested case-control study we compared participants with extreme phenotype selected from Italian GEN-COVID cohort SARS-CoV-2-infected (<60 y, 79 severe cases versus 77 control cases). applied LASSO Logistic...
Abstract In recent months, Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world. COVID-19 patients show mild, moderate or symptoms with latter ones requiring access to specialized intensive care. SARS-CoV-2 infections, pathogenesis and progression have not been clearly elucidated yet, thus forcing development of many complementary approaches identify candidate cellular pathways involved in progression. Host...
Abstract Quantitative phase imaging has gained popularity in bioimaging because it can avoid the need for cell staining, which, some cases, is difficult or impossible. However, as a result, quantitative does not provide labelling of various specific intracellular structures. Here we show novel computational segmentation method based on statistical inference that makes possible techniques to identify nucleus. We demonstrate approach with refractive index tomograms stain-free cells...
Abstract The response to antiviral therapy is lower in hepatitis C virus (HCV) patients with genotype 1 than those 2. Overexpression of the suppressor cytokine signaling 3 (SOCS3) gene liver tissue associated a poorer treatment outcome chronic viral 1. Also, insulin resistance has been implicated nonresponse an anti-HCV treatment. To understand why HCV respond differently, we investigated SOCS3 expression, metabolic syndrome (MS), and cohort HCV-related hepatitis. A total 198 (108 90 2)...
Neuroblastoma is a malignant neoplasm of the developing sympathetic nervous system that notable for its phenotypic diversity. High-risk patients typically have widely disseminated disease at diagnosis and poor survival probability, but low-risk frequently localized tumors are almost always cured with little or no chemotherapy. Our genome-wide association study (GWAS) has identified common variants within FLJ22536, BARD1, LMO1 as significantly associated neuroblastoma more robustly high-risk...
TP53 is the most frequently mutated gene in human malignancies; however, de novo somatic mutations childhood embryonal cancers such as neuroblastoma are rare. We report on analysis of three independent case-control cohorts comprising 10290 individuals and demonstrate that rs78378222 rs35850753, rare germline variants linkage disequilibrium map to 3' untranslated region (UTR) 5' UTR Δ133 isoform TP53, respectively, robustly associated with (rs35850753: odds ratio [OR] = 2.7, 95% confidence...
Several neuroblastoma (NB) susceptibility loci have been identified within LINC00340, BARD1, LMO1, DUSP12, HSD17B12, DDX4, IL31RA, HACE1 and LIN28B by genome-wide association (GWA) studies including European American individuals. To validate comprehensively evaluate the impact of NB variants on disease risk phenotype, we analyzed 16 single nucleotide polymorphisms (SNPs) in an Italian population (370 cases 809 controls). We assessed their regulatory activity gene expression lymphoblastoid...
HIF1A (Hypoxia-Inducible-Factor 1A) expression in solid tumors is relevant to establish resistance therapeutic approaches. The use of compounds direct against hypoxia signaling and does not show clinical efficiency because changeable oxygen concentrations tumor areas. identification targets expressed both normoxia HIF1A/hypoxia signatures might meliorate the prognostic stratification successes patients with high-risk tumors. In this study, we conducted a combined analysis RNA DNA methylation...
Abstract Circulating tumor cells (CTCs) are rare released from primary, metastatic, or recurrent tumors in the peripheral blood of cancer patients. CTCs isolation and their molecular characterization represent a new marker screening, diagnostic tool called “liquid biopsy” (LB). Compared to traditional tissue biopsy that is invasive does not reveal heterogeneity, LB noninvasive reflects “real‐time” dynamism drug sensitivity. In frame LB, paradigm based on single‐cell label‐free analysis...
The established risk factors of coronavirus disease 2019 (COVID-19) are advanced age, male sex, and comorbidities, but they do not fully explain the wide spectrum manifestations. Genetic implicated in host antiviral response provide for novel insights into its pathogenesis.We performed an in-depth genetic analysis chromosome 21 exploiting genome-wide association study data, including 6,406 individuals hospitalized COVID-19 902,088 controls with European ancestry from Host Genetics...
COVID-19 is a global threat that has spread since the end of 2019, causing severe clinical sequelae and deaths, in context world pandemic. The infection highly pathogenetic infectious SARS-CoV-2 coronavirus been proven to exert systemic effects impacting metabolism. Yet, metabolic pathways involved pathophysiology progression are still unclear. Here, we present results mass spectrometry-based targeted metabolomic analysis on cohort 52 hospitalized patients, classified according disease...
Abstract Toll-like receptors (TLR) are crucial components in the initiation of innate immune responses to a variety pathogens, triggering production pro-inflammatory cytokines and type I II interferons, which responsible for antiviral responses. Among different TLRs, TLR7 recognizes several single-stranded RNA viruses including SARS-CoV-2. We others identified rare loss-of-function variants X-chromosomal young men with severe COVID-19 no prior history major chronic diseases, that were...
Abstract Image-based identification of circulating tumor cells in microfluidic cytometry condition is one the most challenging perspectives Liquid Biopsy scenario. Here we show a machine learning-powered tomographic phase imaging flow system capable to provide high-throughput 3D phase-contrast tomograms each single cell. In fact, that discrimination against white blood potentially achievable with aid artificial intelligence label-free flow-cyto-tomography method. We propose hierarchical...
Abstract The mechanisms underlying genetic susceptibility at loci discovered by genome-wide association study (GWAS) approaches in human cancer remain largely undefined. In this study, we characterized the high-risk neuroblastoma BRCA1-related locus, BARD1, showing that disease-associated variations correlate with increased expression of oncogenically activated isoform, BARD1β. cells, silencing BARD1β showed genotype-specific cytotoxic effects, including decreased substrate-adherence,...
Objective Adiponectin is an adipocytokine that exerts beneficial effects on obesity and related disorders by two receptors (ADIPORs). produced as a monomer circulates in serum different oligomers. The oligomerization state the tissue expression of adiponectin ADIPORs are linked to its biological activities. In this study, levels total oligomers were evaluated relation surgical weight loss. was analyzed visceral subcutaneous adipose tissues obese patients. Design methods 25 patients 44 age-...
Epigenetic changes in DNA methylation could regulate the expression of several allergy-related genes. We investigated whether tolerance acquisition children with immunoglobulin E (IgE)-mediated cow's milk allergy (CMA) is characterized by a specific profile Th2 (IL-4, IL-5) and Th1 (IL-10, IFN-γ)-associated cytokine genes.DNA CpGs promoting regions genes from peripheral blood mononuclear cells serum level IL-4, IL-5, IL-10 INF-γ were assessed active IgE-mediated CMA (group 1), who acquired...
The spectrum of somatic mutation the most aggressive forms neuroblastoma is not completely determined. We sought to identify potential cancer drivers in clinically neuroblastoma.Whole exome sequencing was conducted on 17 germline and tumor DNA samples from high-risk patients with adverse events within 36 months diagnosis (HR-Event3) mutations deep targeted 134 genes selected initial screening additional 48 pairs (62.5% HR-Event3 patients), tumors human-derived cell lines.We revealed 22...