A5054913846- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Microfluidic and Bio-sensing Technologies
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Metabolism and Genetic Disorders
- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- RNA and protein synthesis mechanisms
- Glioma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Advanced Fluorescence Microscopy Techniques
- Image Processing Techniques and Applications
- Cancer Research and Treatments
- MicroRNA in disease regulation
- Coenzyme Q10 studies and effects
- Cancer-related molecular mechanisms research
- Metabolism, Diabetes, and Cancer
- Endometrial and Cervical Cancer Treatments
- Single-cell and spatial transcriptomics
- Extracellular vesicles in disease
University of Bologna
2016-2025
Azienda USL di Bologna
2025
Istituti di Ricovero e Cura a Carattere Scientifico
2025
The Francis Crick Institute
2017-2020
Shanghai Jiao Tong University
2020
Policlinico S.Orsola-Malpighi
2008-2018
Medica (Italy)
2008-2018
Aarhus University Hospital
2016
Institute of Cancer Research
2016
A. O. Ordine Mauriziano di Torino
2013
Significance Increasing evidence suggests that extracellular vesicles (EVs) can transfer genetic material to recipient cells. However, the mechanism and role of this phenomenon are largely unknown. Here we have made a remarkable discovery: EVs harbor full mitochondrial genome. These in turn their mtDNA cells with impaired metabolism, leading restoration metabolic activity. We determined hormonal therapy induces oxidative phosphorylation-deficient breast cancer cells, which be rescued via...
Abstract Circulating tumor cells (CTCs) are rare released from primary, metastatic, or recurrent tumors in the peripheral blood of cancer patients. CTCs isolation and their molecular characterization represent a new marker screening, diagnostic tool called “liquid biopsy” (LB). Compared to traditional tissue biopsy that is invasive does not reveal heterogeneity, LB noninvasive reflects “real‐time” dynamism drug sensitivity. In frame LB, paradigm based on single‐cell label‐free analysis...
Abstract Image-based identification of circulating tumor cells in microfluidic cytometry condition is one the most challenging perspectives Liquid Biopsy scenario. Here we show a machine learning-powered tomographic phase imaging flow system capable to provide high-throughput 3D phase-contrast tomograms each single cell. In fact, that discrimination against white blood potentially achievable with aid artificial intelligence label-free flow-cyto-tomography method. We propose hierarchical...
DNAJC15 is a mitochondrial TIMM23-related co-chaperonin known for its role in regulating oxidative phosphorylation efficiency, stress response and lipid metabolism. Recently, it has been proposed that the loss of correlates with cisplatin (CDDP)-resistance onset ovarian cancer (OC), suggesting this protein as potential prognostic factor during OC progression. However, molecular mechanisms through which contributes to CDDP remains poorly investigated. Here, we show high levels are associated...
The oncogenic versus suppressor roles of mitochondrial genes have long been debated. Peculiar features genetics such as hetero/homoplasmy and mutation threshold are seldom taken into account in this debate. Mitochondrial DNA (mtDNA) mutations generally claimed to be protumorigenic, but they also hallmarks mostly benign oncocytic tumors wherein help reduce adaptation hypoxia by destabilizing hypoxia-inducible factor-1α (HIF1α). To determine the influence a disassembling mtDNA its on...
Mitochondrial DNA mutations are currently investigated as modifying factors impinging on tumor growth and aggressiveness, having been found in virtually all cancer types most commonly affecting genes encoding mitochondrial complex I (CI) subunits. However, it is still unclear whether they exert a pro- or anti-tumorigenic effect. We here analyzed the impact of three homoplasmic mtDNA (m.3460G>A/MT-ND1, m.3571insC/MT-ND1 m.3243A>G/MT-TL1) osteosarcoma progression, chosen since induce different...
Abstract Background Aerobic glycolysis, namely the Warburg effect, is main hallmark of cancer cells. Mitochondrial respiratory dysfunction has been proposed to be one major causes for such glycolytic shift. This hypothesis revisited as tumors appear undergo waves gene regulation during progression, some which rely on functional mitochondria. In this framework, role mitochondrial complex I still debated, in particular with respect effect DNA mutations metabolism. The aim work provide proof...
Abstract Converting carcinomas in benign oncocytomas has been suggested as a potential anti-cancer strategy. One of the oncocytoma hallmarks is lack respiratory complex I (CI). Here we use genetic ablation this enzyme to induce indolence two cancer types, and show reversed by allowing stabilization Hypoxia Inducible Factor-1 alpha (HIF-1α). We further that on long run CI-deficient tumors re-adapt their inability respond hypoxia, concordantly with persistence human oncocytomas. demonstrate...
The most recent discoveries in the biochemical field are highlighting increasingly important role of lipid droplets (LDs) several regulatory mechanisms living cells. LDs dynamic organelles and therefore their complete characterization terms number, size, spatial positioning relative distribution cell volume can shed light on roles played by LDs. Until now, fluorescence microscopy transmission electron assessed as gold standard methods for identifying due to high sensitivity specificity....
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive neoplasia, characterized by early metastasis, low diagnostic rates at stages, resistance to drugs, and poor prognosis. There an urgent need better characterize this disease in order identify efficient diagnostic/prognostic biomarkers. Since microRNAs (miRNAs) contribute oncogenesis metastasis formation PDAC, they are considered potential candidates for fulfilling task. In work, levels two miRNA subsets (involved...
RET is a tyrosine kinase transmembrane receptor expressed in two main alternative isoforms: RET9 and RET51. transduces positive signal leading to survival, differentiation, or migration the presence of its ligand glial cell line-derived neurotrophic factor, whereas absence proapoptotic fragment that initiates negative signaling for apoptosis generated. The transduction mechanisms are still unclear.To shed light on RET-induced apoptosis, we searched novel interactors RET51.The "split...
Mitochondrial DNA (mtDNA) mutations have been described in almost all types of cancer. However, their exact role and timing occurrence during tumor development progression are still a matter debate. A Vogelstein-like model is well established for endometrial carcinoma (EC), however, mtDNA has scarcely investigated these tumors despite the fact that mitochondrial biogenesis increase shown to be hallmark type I EC. Here, we screened panel 23 EC tissues matched typical hyperplasia four...
Mitochondrial DNA (mtDNA) mutations leading to the disruption of respiratory complex I (CI) have been shown exhibit anti-tumorigenic effects, at variance with those impairing only function but not assembly complex, which appear contribute positively cancer development. Owing challenges in analysis multi-copy mitochondrial genome, it is yet be determined whether tumour-associated mtDNA lesions occur as somatic modifying factors or germ-line predisposing elements. Here we investigated whole...
Mitochondrial biogenesis is an orchestrated process that presides to the regulation of organelles homeostasis within a cell. We show γ-rays, at doses commonly used in radiation therapy for cancer treatment, induce increase mitochondrial mass and function, response genotoxic stress pushes cells into senescence, presence functional p53. Although main effector γ-rays p53-p21 axis, we demonstrated only indirectly regulated by p53, whose activation triggers murine double minute 2 (MDM2)-mediated...
Cytochrome b is the only mtDNA-encoded subunit of mitochondrial complex III (CIII), functional bottleneck respiratory chain. Previously, human cytochrome missense mutation m.15579A>G, which substitutes Tyr 278 with Cys (p.278Y>C), was identified in a patient severe exercise intolerance and multisystem manifestations. In this study, we characterized biochemical properties cybrids carrying report that homoplasmic p.278Y>C caused dramatic reduction CIII activity CIII-driven ATP synthesis....
To efficiently tackle certain tumor types, finding new biomarkers for rapid and complete phenotyping of cancer cells is highly demanded. This especially the case most common pediatric solid sympathetic nervous system, namely, neuroblastoma (NB). Liquid biopsy in principle a very promising tool this purpose, but usually enrichment isolation circulating such patients remain difficult due to unavailability universal NB cell-specific surface markers. Here, we show that screening through...
A disruptive frameshift mtDNA mutation affecting the ND5 subunit of complex I is present in homoplasmy a nasopharyngeal oncocytic tumor and inherited as heteroplasmic germline recurring two patient's siblings. Homoplasmic correlates with lack ND6 subunit, suggesting disassembly. few areas, expressing for mutation, harbor de novo homoplasmic ND1 mutation. Since shift to mutations occurs exclusively cells, we conclude that may have selective advantage accompany transformation. Hum Mutat 0,...
Glioblastoma multiforme (GBM) is the most malignant brain tumor in adults, but its etiology still remains unknown. Recently, a role of viruses such as cytomegalovirus and JC virus gliomagenesis has been suggested. Since human papillomavirus (HPV) considered common oncogenic humans, we evaluated occurrence GBM samples. Fifty-two formalin-fixed paraffin-embedded primary glioblastoma specimens were retrospectively analyzed. The presence HPV genome on DNA was assessed by MY/GP nested PCR....
Thyroid neoplasias with oncocytic features represent a specific phenotype in non-medullary thyroid cancer, reflecting the unique biological phenomenon of mitochondrial hyperplasia cytoplasm. Oncocytic cells are characterized by prominent eosinophilia (or oxyphilia) caused abundance. Although disruptive mutations DNA (mtDNA) most significant hallmark such tumors, oncocytomas may be envisioned as heterogeneous neoplasms, multiple nuclear and gene lesions. We investigated mutational profile...