A5012779792- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Hepatitis C virus research
- Digital Holography and Microscopy
- Reproductive Biology and Fertility
- HIV/AIDS Research and Interventions
- Hepatitis B Virus Studies
- Sperm and Testicular Function
- Microfluidic and Bio-sensing Technologies
- Genetic and phenotypic traits in livestock
- Immune Cell Function and Interaction
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- HIV-related health complications and treatments
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Cardiovascular Syncope and Autonomic Disorders
- Effects of Environmental Stressors on Livestock
- Orbital Angular Momentum in Optics
- Cytomegalovirus and herpesvirus research
- Liver Disease Diagnosis and Treatment
- Fish Ecology and Management Studies
- Advanced Optical Imaging Technologies
- Quantum Chromodynamics and Particle Interactions
- Plant and animal studies
University of Copenhagen
2013-2024
Copenhagen University Hospital
2011-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2010-2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2014-2023
Vita-Salute San Raffaele University
2004-2023
Technical University of Denmark
2022
Hvidovre Hospital
2014-2021
San Raffaele University of Rome
2006-2021
Associazione Regionale Allevatori della Lombardia
2020-2021
Cereal Research Centre
2019-2020
Objective: We compared the immunological and clinical outcomes of lamivudine monotherapy complete therapy interruption in treatment HIV-1-infected patients harbouring lamivudine-resistant virus. Methods: This 48-week, open-label pilot study randomly assigned HIV-infected receiving lamivudine-containing HAART M184V mutation to with 300 mg once daily (lamivudine group) or discontinuation all antiretroviral drugs (TI group). The primary endpoint was occurrence failure; failure defined as first...
Sperm morphology is regarded as a significant prognostic factor for fertilization, abnormal sperm structure one of the most common factors in male infertility. Furthermore, obtaining accurate morphological information an important issue with strong implications zoo-technical industries, example to perform sorting species X from Y. A challenging step forward would be availability fast, high-throughput and label-free system measurement physical parameters visualization 3D shape such biological...
Efforts to mitigate the coronavirus disease 2019 (COVID-19) pandemic include screening of existing antiviral molecules that could be repurposed treat severe acute respiratory syndrome 2 (SARS-CoV-2) infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nuc’s) often show decreased activity these cells due inefficient metabolization.
We show that sharp imaging and quantitative phase-contrast microcopy is possible in microfluidics flowing turbid media by digital holography. In fact, liquids with suspended colloidal particles, clear vision hindered cannot be recovered any other microscopic technique. On the contrary, using holography, thanks to Doppler frequency shift experienced photons scattered which do not contribute interference process, i.e. recorded hologram. The method illustrated results are demonstrated for pure...
The COVID-19 pandemic continues to threaten healthcare systems worldwide due the limited access vaccines, suboptimal treatment options, and continuous emergence of new more transmissible SARS-CoV-2 variants. Reverse-genetics studies viral genes mutations have proven highly valuable in advancing basic virus research, leading development therapeutics. We developed a functional versatile full-length infectious system by cloning sequence associated isolate (DK-AHH1) into bacterial artificial...
Differential image contrast (DIC), through the numerical managing and manipulation of complex wavefronts obtained by digital holography (DH), is investigated.We name approach Dynamical Holographic Image Contrast (DDHIC).DDHIC dispenses from special optics and/or setup configurations with moveable components, as usually occurs in classical DIC, that not well-suited for investigating objects experiencing dynamic evolution during measurement.In fact, technique presented here, useful floating...
: Evaluate HIV-1 subtype B integrase gene evolution in patients failing raltegravir (RAL)-based savage regimens by clonal analysis of the replicating viral quasispecies.: Seven triple class failure (subtype B)-infected patients, followed at San Raffaele Hospital and enrolled RAL Expanded Access Program (MK0518-023), were evaluated. Patients up for 24-48 weeks due to absence other active drugs, was maintained their even if resistance mutations detected.: Immunologic virologic parameters...
An investigation is reported of the identification and measurement region interest (ROI) in quantitative phase-contrast maps biological cells by digital holographic microscopy. In particular, two different methods have been developed for vitro bull sperm head morphometry analysis. We show that semen analysis can be accomplished means proposed techniques . Extraction various parameters are performed. It demonstrated both efficient to skim data set a preselective discarding anomalous data.
The preparation of sexed semen is based on the differential DNA content between X and Y chromosome bearing sperm cells determined by fluorescence-activated cell sorting. In spite its intrinsic limitations this represents only effective method. However, employment for breeding food producing animals a large scale requires additional knowledge in protein repertoire development improved methods to differentiate maintaining high vitality. order address issue, we performed comparative shotgun...
In the last few decades a negative association between level of milk production and fertility has been observed. Currently, most utilized method measuring male employed by livestock industry is related to Non-Return Rate (NRR). Through differential proteome analysis, this study evaluated changes in expression protein profile spermatozoa collected from 16 bulls with different levels field expressed as an estimated relative conception rate (ERCR). The main aim identify putative markers be used...
Significance HIV-1 full-length RNA must go to specific subcellular compartments carry out its functions as a template for translation of structural and enzymatic proteins the genetic material new virions. mislocalization can affect encoded proteins, causing defects in viral replication. Currently, little is known about how transported cytoplasm. Here, we demonstrate that RNAs use diffusion major mechanism cytoplasmic transport absence group-specific antigen (Gag) even presence sufficient Gag...
Significance Hepatitis C virus (HCV) is a pervasive human pathogen for which vaccine urgently needed. Vaccine development relies on inducing neutralizing antibodies (NAbs), but HCV employs complex mechanisms to evade neutralization that remain incompletely understood. Here, we discover unique interplay between two separate molecular features of envelope protein E2, the hypervariable region 1 and N-linked glycans, protect from NAbs. Furthermore, find they share mechanism in broadly modulate...
A targeted survey was designed with the aim of describing diffusion precision livestock farming (PLF) tools in one most intensive dairy provinces Italy. Technicians at Provincial Breeder Association Cremona interviewed 490 farmers and obtained data regarding role age respondents; land owned by farmers; their herd sizes (HS, lactating plus dry cows; small HS < 101, medium 101-200, large > 200 cows/herd); average 305 day milk yield (low MY 9501, 9501-10,500, high 10,500 kg/head); cow to...
Antivirals targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could improve treatment of COVID-19. We evaluated the efficacy clinically relevant hepatitis C virus (HCV) NS3 protease inhibitors (PIs) against SARS-CoV-2 and their interactions with remdesivir, only direct-acting antiviral approved for COVID-19 treatment.
A digital holographic microscope (DHM) has been employed in the retrieval and analysis of morphological images bovine's sperm cells. Digital holography is a noncontact technique capable investigating shape sample without altering its characteristics used for first time retrieving quantitative information Different spermatozoa have analyzed by means this allowing us to obtain 3-D with precise topographical details valuable about defects, provided biological considerations. Moreover, making...
HIV-1 non-B subtypes have recently entered Western Europe following immigration from other regions. The distribution of clades and their association with demographic factors, over the entire course epidemic, not been fully investigated in Italy.We carried out a phylogenetic analysis pol sequences derived 3670 patients followed at 50 Italian clinical centres nearly three decades.Overall, 417 (11.4%) subtypes. prevalence strains increased 2.6% 1980-1992 to 18.9% 1993-2008 (P<0.0001) subset...
Genetic recombination is an important mechanism for increasing diversity of RNA viruses, and constitutes a viral escape to host immune responses treatment with antiviral compounds. Although rare, epidemiologically hepatitis C virus (HCV) recombinants have been reported. In addition, regulatory cytopathogenicity the related pestiviruses. Here we describe HCV in cell culture leading production infectious virus. Initially, hepatoma cells were co-transfected replicating JFH1ΔE1E2 genome...