A5031824210- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Hepatitis B Virus Studies
- HIV/AIDS drug development and treatment
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Animal Virus Infections Studies
- COVID-19 epidemiological studies
- Biochemical and Molecular Research
- COVID-19 Clinical Research Studies
- Infective Endocarditis Diagnosis and Management
- Viral Infections and Outbreaks Research
- Pneumonia and Respiratory Infections
- Biosensors and Analytical Detection
- Polar Research and Ecology
- COVID-19 Impact on Reproduction
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Marine Biology and Ecology Research
- Influenza Virus Research Studies
Hvidovre Hospital
2017-2024
Copenhagen University Hospital
2017-2024
Rigshospitalet
2021-2024
University of Copenhagen
2017-2023
Roskilde University
2002-2023
Metropolitan University
2023
Amager Hospital
2021-2022
Bipar
2018
Gentofte Hospital
2018
Following emergence of the SARS-CoV-2 variant Omicron in November 2021, dominant BA.1 sub-lineage was replaced by BA.2 Denmark. We analysed first 2,623 cases from 29 2021 to 2 January 2022. No epidemiological or clinical differences were found between individuals infected with versus BA.2. Phylogenetic analyses showed a geographic east-to-west transmission Capital Region clusters expanding after Christmas holidays. Mutational analysis shows distinct and
New lineages of SARS-CoV-2 are potential concern due to higher transmissibility, risk severe outcomes, and/or escape from neutralizing antibodies. Lineage B.1.1.7 (the Alpha variant) became dominant in early 2021, but the association between transmissibility and factors, such as age primary case viral load remains poorly understood. Here, we used comprehensive administrative data Denmark, comprising full population (January 11 February 7, 2021), estimate household transmissibility. This...
Efforts to mitigate the coronavirus disease 2019 (COVID-19) pandemic include screening of existing antiviral molecules that could be repurposed treat severe acute respiratory syndrome 2 (SARS-CoV-2) infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nuc’s) often show decreased activity these cells due inefficient metabolization.
In Denmark, vaccination against Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) has been with the Pfizer-BioNTech (BTN162b2) or Moderna (mRNA-1273) mRNA vaccines. Patients chronic hepatitis C virus (HCV) infection followed in our clinic received vaccinations according to Danish roll-out plan. To monitor HCV infection, RNA was extracted from patient plasma and sequencing performed on Illumina platform. 10 of 108 samples, full-length traces SARS-CoV-2 spike vaccine sequences were...
bla TEM-1 encodes a narrow-spectrum β-lactamase that is inhibited by inhibitors and commonly present in Escherichia coli. Hyperproduction of blaTEM-1 may cause resistance to penicillin/β-lactamase inhibitor (P/BLI) combinations.To characterize EC78, an E. coli bloodstream isolate, resistant P/BLI combinations, which contains extensive amplification within the chromosome.EC78 was sequenced using Illumina Oxford Nanopore Technology (ONT) methodology. Configuration probed PCR. Expression mRNA...
Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has been associated with a high risk of adverse outcomes in solid organ transplant (SOT) recipients the pre-vaccination era. In this retrospective cohort study, we examined incidence and severity COVID-19 kidney liver Denmark post-vaccination era, from December 27, 2020, to 2021. We included 1428 SOT 143 cases first-positive PCR test. The cumulative test 1 year after initiation vaccination was 10.4% (95% CI: 8.8-12.0), higher than...
Abstract Background In early 2021, the SARS-CoV-2 lineage B.1.1.7 (Alpha variant) became dominant across large parts of world. Denmark, comprehensive and real-time test, contact-tracing, sequencing efforts were applied to sustain epidemic control. Here, we use these data investigate transmissibility, introduction, onward transmission in Denmark. Methods We analyzed a set 60,178 genomes generated from high-throughput by Danish COVID-19 Genome Consortium, representing 34% all positive cases...
We describe a ceftriaxone-resistant Salmonella Typhi bacteraemia in pregnant woman returning from family visit Pakistan. Whole genome sequencing confirmed similarity to Pakistani outbreak clone. Pregnancy and unawareness of this delayed appropriate antibiotic therapy. Concurrently, we detected faecal carriage carbapenemase-producing Escherichia coli. Awareness the ongoing should affect empiric treatment typhoid fever hygiene precautions travellers Meropenem may be warranted severe cases.
In 2020, the novel coronavirus, SARS-CoV-2, caused a pandemic, which is still raging at time of writing this. Here, we present results from SpikeSeq, first published Sanger sequencing-based method for detection Variants Concern (VOC) and key mutations, using 1 kb amplicon recognized ARTIC Network primers. The proposed setup relies entirely on materials methods already in use diagnostic RT-qPCR labs existing commercial infrastructure offering sequencing services. For data analysis, provide an...
Primary screening for VRE with PCR directed against vanA allowed identification of vanA+ samples from which could not be isolated when selective culture methods were used. From such a sample vancomycin-susceptible, Enterococcus faecium, Efm-V1511, was isolated, vancomycin selection used during culture. Similar isolates variable susceptibility to obtained in the following months.To characterize Efm-V1511 and investigate causes vancomycin.All strains sequenced using Illumina technology....
Objectives: We describe factors associated with and barriers to initiation of Direct Acting Antiviral (DAA) treatment in patients chronic hepatitis C, who fulfill national fibrosis guidelines Denmark.Materials Methods: In this nationwide cohort study, we included C from The Danish Database for Hepatitis B (DANHEP) fulfilled criteria. Factors failure were determined by logistic regression analyses. Medical records reviewed criteria, but did not initiate DAA determine the cause.Results: 344...
Synbiotics combine probiotics and prebiotics are being investigated for potential health benefits. In this single-group-design trial, we analyzed changes in the gut microbiome, stool quality, gastrointestinal well-being 15 healthy volunteers after a synbiotic intervention comprising Lacticaseibacillus rhamnosus (LGG), Lactobacillus acidophilus (LA-5), paracasei subsp. (L. CASEI 431), Bifidobacterium animalis lactis BB-12 20 g of chicory-derived inulin powder consumed daily 4 weeks. Fecal...
Abstract Background Since 2012, the incidence of vancomycin-resistant Enterococcus faecium (VREfm) has increased dramatically in Copenhagen and vanA E. become endemic polyclonal. Objectives To examine whether a patient with positive VRE clinical sample had same VREfm preceding screening (within 60 days). Methods We performed 30 month retrospective study. From our laboratory information system (LIS), we identified all patients an invasive isolate rectal within days before infection. pairs...
Abstract Daptomycin is a cyclic lipopeptide used in the treatment of vancomycin-resistant Enterococcus faecium (VREfm). However, development daptomycin-resistant VREfm challenges nosocomial infections. Resistance mechanisms daptomycin are not fully understood. Here, we analyzed genomic changes leading to daptomycin-susceptible isolate becoming resistant after 50 days and linezolid combination therapy. A total seven isogenic isolates from same patient (daptomycin-susceptible...
Vast amounts of pathogen genomic, demographic and spatial data are transforming our understanding SARS-CoV-2 emergence spread. We examined the drivers molecular evolution spread 291,791 genomes from Denmark in 2021. With a sequencing rate consistently exceeding 60%, up to 80% PCR-positive samples between March November, viral genome set is broadly whole-epidemic representative. identify consistent rise diversity over time, with notable spikes upon importation novel variants (e.g., Delta...
Objective HCV-genotype 4 infections are a major cause of liver diseases in the Middle East/Africa with certain subtypes associated increased risk direct-acting antiviral (DAA) treatment failures. We aimed at developing infectious genotype cell culture systems to understand evolutionary genetic landscapes resistance, which can help preserve future efficacy DAA-based therapy. Design HCV recombinants were tested liver-derived cells. Long-term coculture DAAs served induce antiviral-resistance...
Shortening the treatment duration for chronic hepatitis C may increase feasibility and reduce cost of cure. The aims this study were to compare 4 weeks glecaprevir/pibrentasvir (GLE/PIB) with without ribavirin patients favourable baseline characteristics monitor development resistance-associated substitutions (RAS) re-treatment outcomes if failed.We performed an open-label single-centre randomized controlled trial, in which 1:1 GLE/PIB ± ribavirin, stratified by genotype 3. main inclusion...
In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and stronger positive subtype A(H3N2). Forty-three were in InfA (H3N2) (mean Cq 30.9, range 23.9-35.1), 26 of 43 28.0, 23.2-30.6). Our multiplex test is laboratory-developed four-target, four-color reverse-transcription PCR assay targeting gene, subtypes A(H3N2) A(H1N1)pdm09. Several when retested on commercial Point-of-Care assays. As stand-alone target most diagnostic...