Lars Christoffersen
A5000739494- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Bacterial biofilms and quorum sensing
- Protein Structure and Dynamics
- Cystic Fibrosis Research Advances
- Cell Image Analysis Techniques
- Animal Virus Infections Studies
- Single-cell and spatial transcriptomics
- SARS-CoV-2 detection and testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Maritime and Coastal Archaeology
- Inhalation and Respiratory Drug Delivery
- Historical and Architectural Studies
- Mycobacterium research and diagnosis
- Enzyme Structure and Function
- COVID-19 epidemiological studies
- Gene Regulatory Network Analysis
- Machine Learning in Bioinformatics
- Plant Virus Research Studies
Statens Serum Institut
2021-2025
University of Copenhagen
2018-2022
Rigshospitalet
2007-2014
Quorum sensing (QS) denotes a density-dependent mode of inter-bacterial communication based on signal transmitter molecules. Active QS is present during chronic infections with the opportunistic pathogen Pseudomonas aeruginosa in immunocompromised patients. The authors have previously demonstrated QS-regulated tolerance biofilm bacteria to antimicrobial properties polymorphonuclear leukocytes (PMNs). precise effect PMNs is, however, unknown. Incubation human supernatants from dense P....
Cystic fibrosis (CF) patients have increased susceptibility to chronic lung infections by Pseudomonas aeruginosa, but the ecophysiology within CF during is poorly understood. The aim of this study was elucidate in vivo growth physiology P. aeruginosa lungs chronically infected patients. A novel, quantitative peptide nucleic acid (PNA) fluorescence situ hybridization (PNA-FISH)-based method used estimate rates directly tissue samples from and a mouse model. rate did not correlate with...
New lineages of SARS-CoV-2 are potential concern due to higher transmissibility, risk severe outcomes, and/or escape from neutralizing antibodies. Lineage B.1.1.7 (the Alpha variant) became dominant in early 2021, but the association between transmissibility and factors, such as age primary case viral load remains poorly understood. Here, we used comprehensive administrative data Denmark, comprising full population (January 11 February 7, 2021), estimate household transmissibility. This...
Summary Single-cell RNA-seq (scRNA-seq) assays are being increasingly utilized to investigate specific hypotheses in both basic biology and clinically-applied studies. The design of most such studies can be often reduced a comparison between two or more groups samples, as disease cases healthy controls, treatment placebo. Comparative analysis scRNA-seq samples brings additional statistical considerations, currently there is lack tools address this common scenario. Based on our experience...
Abstract Background In early 2021, the SARS-CoV-2 lineage B.1.1.7 (Alpha variant) became dominant across large parts of world. Denmark, comprehensive and real-time test, contact-tracing, sequencing efforts were applied to sustain epidemic control. Here, we use these data investigate transmissibility, introduction, onward transmission in Denmark. Methods We analyzed a set 60,178 genomes generated from high-throughput by Danish COVID-19 Genome Consortium, representing 34% all positive cases...
Background/Objectives: Fosfomycin, a critically important antibiotic, is widely used to treat urinary tract infections (UTIs) caused by multidrug-resistant (MDR) Escherichia coli, particularly those producing extended-spectrum β-lactamases (ESBLs). However, its increasing use in livestock has raised concerns about resistance development and global dissemination. This study investigated fosfomycin E. coli isolates from 400 fecal samples collected at Brazilian broiler farms. Methods: The were...
Based on the development of new algorithms and growth sequence databases, it has recently become possible to build robust higher-order models based sets aligned protein sequences. Such have proven useful in de novo structure prediction, where are used find pairs residues that co-vary during evolution, hence likely be spatial proximity native protein. The accuracy these algorithms, however, drop dramatically when number sequences alignment is small. We developed a method we termed CE-YAPP...
Abstract Based on the development of new algorithms and growth sequence databases, it has recently become possible to build robust informative higher-order statistical models based large sets aligned protein sequences. By disentangling direct indirect effects, such have proven useful assess phenotypic landscapes, determine protein-protein interaction sites, in de novo structure prediction. In context prediction, are used find pairs residues that co-vary during evolution, hence likely be...