A5074555605- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Tracheal and airway disorders
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Asthma and respiratory diseases
- Child Nutrition and Feeding Issues
- Infections and bacterial resistance
- Plant Pathogenic Bacteria Studies
- Respiratory viral infections research
- Immunodeficiency and Autoimmune Disorders
- Pediatric health and respiratory diseases
- Antifungal resistance and susceptibility
- Nosocomial Infections in ICU
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Bacterial Infections and Vaccines
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Adolescent and Pediatric Healthcare
- Mycobacterium research and diagnosis
- Nematode management and characterization studies
- Pharmaceutical studies and practices
- Fungal Infections and Studies
- Vitamin D Research Studies
Rigshospitalet
2015-2024
Copenhagen University Hospital
2015-2024
Danish Multiple Sclerosis Center
2006-2023
Inserm
2020
École nationale d'ingénieurs de Brest
2020
Université de Bretagne Occidentale
2020
University of Copenhagen
1995-2019
Office of Adolescent Health
2018
Cystic Fibrosis Foundation
1999-2017
KU Leuven
2016
The present study was undertaken to investigate the appearance and location of Pseudomonas aeruginosa in cystic fibrosis (CF) lung sputum. Samples include preserved tissues CF patients who died due chronic P. infection prior advent intensive antibiotic therapy, explanted lungs from 3 intensively treated chronically infected routine sputum 77 patients. All samples were investigated microscopically using hematoxylin-eosin (HE), Gram alcian-blue stain, PNA FISH immunofluorescence for...
Mannose-binding lectin (MBL) is a key factor in innate immunity, and lung infections are the leading cause of morbidity mortality cystic fibrosis (CF). Accordingly, we investigated whether MBL variant alleles, which associated with recurrent infections, might be risk factors for CF patients. In 149 patients, different genotypes were compared respect to function, microbiology, survival end-stage (death or transplantation). The function was significantly reduced carriers alleles when normal...
Quorum sensing (QS) denotes a density-dependent mode of inter-bacterial communication based on signal transmitter molecules. Active QS is present during chronic infections with the opportunistic pathogen Pseudomonas aeruginosa in immunocompromised patients. The authors have previously demonstrated QS-regulated tolerance biofilm bacteria to antimicrobial properties polymorphonuclear leukocytes (PMNs). precise effect PMNs is, however, unknown. Incubation human supernatants from dense P....
Rationale: The triple-combination regimen elexacaftor/tezacaftor/ivacaftor (ELX/TEZ/IVA) was shown to be safe and efficacious in children aged 6 through 11 years with cystic fibrosis at least one F508del-CFTR allele a phase 3, open-label, single-arm study. Objectives: To further evaluate the efficacy safety of ELX/TEZ/IVA age heterozygous for F508del minimal function CFTR mutation (F/MF genotypes) randomized, double-blind, placebo-controlled 3b trial. Methods: Children were randomized...
Oxidative stress caused by chronic lung inflammation in patients with cystic fibrosis (CF) and infection Pseudomonas aeruginosa is characterized the reactive oxygen species (ROS) liberated polymorphonuclear leukocytes (PMNs). We formulated hypothesis that oxidation of bacterial DNA ROS presents an increased risk for occurrence hypermutable P. aeruginosa. The isolates was investigated directly sputum 79 CF among 141 collected from 11 (10 to 15 isolates/patient) 1st up 25th year their...
Respiratory tract colonization by molds in patients with cystic fibrosis (CF) were analyzed, particular focus on the frequency, genotype, and underlying mechanism of azole resistance among Aspergillus fumigatus isolates. Clinical demographic data also analyzed. A total 3,336 respiratory samples from 287 CF collected during two 6-month periods 2007 2009. Azole was detected using an itraconazole screening agar (4 mg/liter) EUCAST method. cyp51A gene sequencing microsatellite genotyping...
This is the second in a series of four papers updating European Cystic Fibrosis Society (ECFS) standards for care people with CF. paper focuses on establishing and maintaining health. The guidance produced using an evidence-based framework wide stakeholder engagement, including from CF community. Authors provided narrative description their topic statements, which were more directive. These statements reviewed by Delphi exercise, achieving good levels agreement group all statements....
Most cystic fibrosis (CF) patients with chronic Pseudomonas aeruginosa lung infection have a persistent acute type inflammation dominated by polymorphonuclear neutrophils (PMN) and pronounced antibody response against P. aeruginosa. We speculated whether this immune in CF is of the Th2 change to Th1 could improve prognosis. Therefore, we studied 14 (CF +P) without –P) infection. The specific production interferon‐gamma (IFN‐γ) interleukin‐4 (IL‐4) peripheral blood mononuclear cells was...
Aerosolized recombinant human deoxyribonuclease (rhDNase I (Pulmozyme)) has previously been shown to increase pulmonary function and reduce exacerbations of respiratory symptoms in cystic fibrosis (CF) patients with moderate severe reduction function.To analyse whether aerosolized Pulmozyme could the number bacterial infections lower airways CF without chronic infection.Patients were randomized either 2(1/2) mg once daily or no rhDNase treatment. The study period was 1 y, blinded for...
Cystic fibrosis (CF) is a life shortening disease, however prognosis has improved and the adult population growing. Most adults with cystic live independent lives balance demands of work family significant treatment burden. The aim this study was to examine relationships among adherence, symptoms depression health-related quality (HRQoL) in young CF.We administered three standardized questionnaires 67 patients CF aged 18-30 years; Morisky Medication Adherence Scale, Major Depression...
Despite intensive antibiotic treatment, Pseudomonas aeruginosa often persists in the airways of cystic fibrosis (CF) patients for decades, and can do so without resistance development. Using high-throughput screening assays bacterial survival after treatment with high concentrations ciprofloxacin, we have determined prevalence persisters a large patient cohort using 460 longitudinal isolates P. from 39 CF patients. Isolates were classed as persister variants (Hip) if they regrew following at...
Achromobacter species are increasingly being detected in patients with cystic fibrosis (CF), and this emerging pathogen is associated antibiotic resistance more-severe disease outcomes. Nonetheless, little known about the extent of transmission development infections. We sequenced genomes 101 clinical isolates (identified as xylosoxidans based on matrix-assister laser desorption ionization-time flight [MALDI-TOF] or API N20 typing) collected from 51 CF-the largest longitudinal data set to...
Deficiency of mannose-binding lectin has been shown to be a risk factor for cystic fibrosis (CF) patients. We, therefore, decided treat patient with CF, deficiency, severe bronchopulmonary Pseudomonas aeruginosa infection, and rapid deterioration lung function purified in an attempt ameliorate the course disease. The used originated from pooled human donor plasma was given as intravenous infusion twice week period 3 months. patients's clinical condition stabilized during treatment period,...
In a 4-year prospective study, we evaluated specific airway resistance (sRaw) by whole-body plethysmography, respiratory the interrupter technique, and reactance at 5 Hz impulse oscillation technique combined with measurement of responsiveness to bronchodilators cold air in 30 children (mean [range] age 5.7 [2 8] years) cystic fibrosis (CF). Spirometry was done school age. Mean sRaw consistently abnormal: mean z score (SD) 2.52 (2.02) (p < 0.001) start unchanged 36 months later 2.74 (2.02)....
Azole resistance in Aspergillus terreus isolates was explored. Twenty related (MB) and 6 unrelated A. were included. CYP51A sequencing RAPD genotyping performed. Five MB itraconazole susceptible, whereas the minimum inhibitory concentrations (MICs) for 15 elevated (1-2 mg/L). Voriconazole posaconazole MICs 0.5-4 0.06-0.5 mg/L, respectively, but 0.25-0.5 <0.03-0.06 controls. Sequencing identified a Cyp51Ap M217I alteration all with MICs. Genotyping showed that 18 of 20 identical unique,...