A5050246861- Bacterial biofilms and quorum sensing
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Cystic Fibrosis Research Advances
- Inhalation and Respiratory Drug Delivery
- Antibiotic Resistance in Bacteria
- Wound Healing and Treatments
- Orthopedic Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Central Venous Catheters and Hemodialysis
- Nosocomial Infections in ICU
- Antibiotics Pharmacokinetics and Efficacy
- Mycobacterium research and diagnosis
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Cardiac Valve Diseases and Treatments
- Neutropenia and Cancer Infections
- Diabetic Foot Ulcer Assessment and Management
- Urinary Tract Infections Management
- S100 Proteins and Annexins
- Infections and bacterial resistance
- Dialysis and Renal Disease Management
Copenhagen University Hospital
2016-2025
Rigshospitalet
2016-2025
University of Copenhagen
2014-2025
European Society of Clinical Microbiology and Infectious Diseases
2024
Zealand University Hospital
2019
Herlev Hospital
2013-2018
Hvidovre Hospital
2018
Innsbruck Medical University
2015
Universität Innsbruck
2015
Université Paris Cité
2014
Biofilms cause chronic infections in tissues or by developing on the surfaces of medical devices. Biofilm persist despite both antibiotic therapy and innate adaptive defence mechanisms patient. are characterized persisting progressive pathology due primarily to inflammatory response surrounding biofilm. For this reason, many biofilm may be difficult diagnose treat efficiently. It is purpose guideline bring current knowledge diagnosis attention clinical microbiologists infectious disease...
Patients with infective endocarditis on the left side of heart are typically treated intravenous antibiotic agents for up to 6 weeks. Whether a shift from oral antibiotics once patient is in stable condition would result efficacy and safety similar those continued treatment unknown.
ABSTRACT Between 1 and 2% of the population in developed world experiences a nonhealing or chronic wound characterized by an apparent arrest stage dominated inflammatory processes. Lately, research groups have proposed that bacteria might be involved contribute to lack healing these wounds. To investigate this, we collected examined samples from wounds obtained 22 different patients, all selected because suspicion Pseudomonas aeruginosa colonization. These were investigated standard...
The opportunistic human pathogen Pseudomonas aeruginosa is the predominant micro-organism of chronic lung infections in cystic fibrosis (CF) patients. P. colonizes CF lungs by forming biofilm structures alveoli. In mode growth bacteria are highly tolerant to otherwise lethal doses antibiotics and protected from bactericidal activity polymorphonuclear leukocytes (PMNs). controls expression many its virulence factors means a cell–cell communication system termed quorum sensing (QS). present...
The opportunistic human pathogen Pseudomonas aeruginosa is the predominant micro-organism of chronic lung infections in cystic fibrosis patients. P. colonizes lungs by forming biofilm microcolonies throughout lung. Quorum sensing (QS) renders bacteria highly tolerant to otherwise lethal doses antibiotics, and protects against bactericidal activity polymorphonuclear leukocytes (PMNs). It has been previously demonstrated that QS inhibited garlic extract. In this study, synergistic effects...
In relation to emerging multiresistant bacteria, development of antimicrobials and new treatment strategies infections should be expected become a high-priority research area. Quorum sensing (QS), communication system used by pathogenic bacteria like Pseudomonas aeruginosa synchronize the expression specific genes involved in pathogenicity, is possible drug target. Previous vitro vivo studies revealed significant inhibition P. QS crude garlic extract. By bioassay-guided fractionation...
Quorum sensing (QS) denotes a density-dependent mode of inter-bacterial communication based on signal transmitter molecules. Active QS is present during chronic infections with the opportunistic pathogen Pseudomonas aeruginosa in immunocompromised patients. The authors have previously demonstrated QS-regulated tolerance biofilm bacteria to antimicrobial properties polymorphonuclear leukocytes (PMNs). precise effect PMNs is, however, unknown. Incubation human supernatants from dense P....
Polymorphonuclear neutrophilic leukocytes (PMNs) play a central role in innate immunity, where they dominate the response to infections, particular cystic fibrosis lung. PMNs are phagocytic cells that produce wide range of antimicrobial agents aimed at killing invading bacteria. However, opportunistic pathogen Pseudomonas aeruginosa can evade destruction by and thus cause persistent infections. In this study, we show biofilm P. recognize presence attracted direct information their fellow...
Many of the virulence factors produced by opportunistic human pathogen Pseudomonas aeruginosa are quorum‐sensing (QS) regulated. Among these rhamnolipids, which have been shown to cause lysis several cellular components immune system, e.g. monocyte‐derived macrophages and polymorphonuclear leukocytes (PMNs). We previously that rhamnolipids P. necrotic death PMNs in vitro . This raises possibility may function as a ‘biofilm shield’ vivo , contributes significantly increased tolerance biofilms...
Chronic lung infection with Pseudomonas aeruginosa is the most severe complication for patients cystic fibrosis (CF). This characterised by endobronchial mucoid biofilms surrounded numerous polymorphonuclear leucocytes (PMNs). The phenotype offers protection against PMNs, which are in general assumed to mount an active respiratory burst leading tissue deterioration. An ongoing PMNs has, however, not been demonstrated previously secretions from chronically infected CF.Based on accumulating...
Cystic fibrosis (CF) patients have increased susceptibility to chronic lung infections by Pseudomonas aeruginosa, but the ecophysiology within CF during is poorly understood. The aim of this study was elucidate in vivo growth physiology P. aeruginosa lungs chronically infected patients. A novel, quantitative peptide nucleic acid (PNA) fluorescence situ hybridization (PNA-FISH)-based method used estimate rates directly tissue samples from and a mouse model. rate did not correlate with...
ObjectivesIn Denmark, the incidence of vancomycin-resistant Enterococcus faecium (VREfm) has increased since 2012. The aim this study was to investigate epidemiology and clonal relatedness VREfm isolates in Danish hospitals 2012–13 using WGS. second evaluate if WGS-based typing could replace PFGE for VREfm.