A5053059399- SARS-CoV-2 detection and testing
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Respiratory viral infections research
- Oral microbiology and periodontitis research
- Biosensors and Analytical Detection
- Salivary Gland Disorders and Functions
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Polyomavirus and related diseases
- Influenza Virus Research Studies
- COVID-19 diagnosis using AI
- Dental Health and Care Utilization
- Animal Virus Infections Studies
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- Parvovirus B19 Infection Studies
- Antimicrobial Resistance in Staphylococcus
- HIV-related health complications and treatments
- Antibiotic Resistance in Bacteria
- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- Chronic Lymphocytic Leukemia Research
- Vaccine Coverage and Hesitancy
Rigshospitalet
2015-2025
Copenhagen University Hospital
2014-2025
University of Copenhagen
1995-2023
Statens Serum Institut
1998-2022
Delta Air Lines (United States)
2022
Imperial College London
2022
Nordsjællands Hospital
2022
Omron (Japan)
2022
Czech Academy of Sciences, Institute of Microbiology
2021
Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to support clinical diagnosis and epidemiological investigations. Recently, large-scale detection of total antibodies (Ab), immunoglobulin G (IgG), IgM against SARS-CoV-2 antigens have been developed, but there limited data on the diagnostic accuracy these assays. This study was a Danish national collaboration evaluated 15 commercial one in-house anti-SARS-CoV-2 in 16 laboratories. Sensitivity...
Background and objectiveThe bacterial profile of saliva is composed bacteria from different oral surfaces. The objective this study was to determine whether diet intake, lifestyle, or socioeconomic status associated with characteristic profiles.DesignStimulated samples 292 participants low levels dental caries periodontitis, enrolled in the Danish Health Examination Survey (DANHES), were analyzed for presence approximately 300 species by means Human Oral Microbe Identification Microarray...
Periodontitis is a multifactorial disease in which subgingival bacteria play an important role the pathogenesis of disease. The objective this study was to determine if periodontitis associated with characteristic salivary bacterial profile. This accomplished by comparing profile saliva from subjects chronic that control cohort.Stimulated samples 139 patients and 447 cohort were analysed using Human Oral Microbe Identification Microarray (HOMIM). Frequency levels (mean HOMIM-value) around...
The purpose of this study was to characterize and compare subgingival salivary microbiotas before after periodontal treatment learn if any changes the microbiota were reflected in saliva. We tested hypothesis that levels specific periopathogens correlate with corresponding treatment.Twenty-five patients generalized chronic periodontitis completed study. Stimulated saliva samples plaque collected at baseline 2, 6, 12 weeks nonsurgical therapy. Subgingival processed by means Human Oral Microbe...
New lineages of SARS-CoV-2 are potential concern due to higher transmissibility, risk severe outcomes, and/or escape from neutralizing antibodies. Lineage B.1.1.7 (the Alpha variant) became dominant in early 2021, but the association between transmissibility and factors, such as age primary case viral load remains poorly understood. Here, we used comprehensive administrative data Denmark, comprising full population (January 11 February 7, 2021), estimate household transmissibility. This...
The chronic Pseudomonas aeruginosa infection of the lungs cystic fibrosis (CF) patients is characterized by biofilm mode growth and inflammation dominated polymorphonuclear leukocytes (PMNs). A high percentage P. strains show frequencies mutations (hypermutators [HP]). exposed to oxygen radicals, both those generated its own metabolism especially released a large number PMNs in response CF lung infection. Our work therefore focused on role DNA oxidative repair system development HP...
Objectives The purpose of this study was to compare microbial profiles saliva, pooled and site-specific subgingival samples in patients with periodontitis. We tested the hypotheses that saliva can be an alternative samples, when screening for presence periopathogens. Design Site specific plaque (n = 54), 18) stimulated were collected from 18 generalized chronic Subgingival salivary microbiotas characterized by means HOMINGS (Human Oral Microbe Identification using Next Generation Sequencing)...
This study compared salivary bacterial profiles in two groups having a 10-fold difference levels of caries experience, as it was hypothesized that the composition microbiota might associate with experience.Bacterial stimulated saliva samples from 85 individuals low experience (healthy group) and 79 high (caries were analyzed by means Human Oral Microbiome Identification Next Generation Sequencing (HOMINGS) technique. Subsequently, caries-free healthy group (n = 57) 31) compared.A...
Recently, reports of severe thromboses, thrombocytopenia, and hemorrhage in persons vaccinated with the chimpanzee adenovirus-vectored vaccine (ChAdOx1 nCoV-19, AZD1222, Vaxzevria; Oxford/AstraZeneca) against acute respiratory syndrome coronavirus 2 have emerged. We describe an otherwise healthy 30-year-old woman who developed ecchymosis, portal vein thrombosis, cerebral venous sinus thrombosis second week after she received ChAdOx1 nCoV-19 vaccine. Extensive diagnostic workup for...
Abstract Background In early 2021, the SARS-CoV-2 lineage B.1.1.7 (Alpha variant) became dominant across large parts of world. Denmark, comprehensive and real-time test, contact-tracing, sequencing efforts were applied to sustain epidemic control. Here, we use these data investigate transmissibility, introduction, onward transmission in Denmark. Methods We analyzed a set 60,178 genomes generated from high-throughput by Danish COVID-19 Genome Consortium, representing 34% all positive cases...
Measles is a highly contagious viral disease. Vaccinated mothers transfer fewer antibodies during pregnancy, resulting in shortened infant immunity. Earlier primary vaccination might avert the gap protection.
Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% the survivors. Erythropoietin (Epo) has - besides its well known haematopoietic properties significant anti-inflammatory, antioxidant anti-apoptotic effects various brain disorders. The neurobiological responses to exogenously injected Epo during murine were examined.Female...
The aim of this study was to learn whether presence caries in an adult population associated with a salivary bacterial profile different from that individuals without untreated caries. Stimulated saliva samples 621 participants the Danish Health Examination Survey were analyzed using Human Oral Microbe Identification Microarray technology. Samples 174 dental and 447 control cohort compared frequency levels identified taxa/clusters as endpoints. Differences at taxon/cluster level...
Abstract Chronic wounds are presumed to persist in the inflammatory state, preventing healing. Emerging evidence indicates a clinical impact of bacterial biofilms soft tissues, including P seudomonas aeruginosa ( PA ) biofilms. To further investigate this, we developed chronic biofilm wound infection model C 3 H / e N and BALB /c mice. The was established by an injection seaweed alginate‐embedded . PAO 1 beneath third‐degree thermal lesion providing full thickness skin necrosis, as human...
The diagnostic yield for cytomegalovirus (CMV) polymerase chain reaction (PCR) viral load in bronchoalveolar lavage (BAL) or plasma to diagnose CMV pneumonia lung transplant recipients remains uncertain and was investigated a large cohort of consecutive recipients.Bronchoscopies within the first year transplantation with detectable BAL by PCR (ie, load, ≥273 IU/mL) were included (66 recipients; 145 bronchoscopies); at each bronchoscopy episode, 2 independent experts reviewed clinical...
Background: Estimates of the severity emerging Omicron variant (lineage B.1.1.529) are critical to assess public health impact associated with its rapid global dissemination. We estimated risk SARS-CoV-2-related hospitalizations after infection compared Delta overall and stratified by vaccination status in Denmark, a country high mRNA coverage an extensive free-of-charge PCR test capacity.Methods: Observational cohort study all RT-PCR SARS-CoV-2 positive individuals Denmark between 21...
Abstract Background and Aims A high prevalence of hepatitis delta virus (HDV) infection, the most severe form viral hepatitis, has been reported among persons living with HIV (PLWH) in Europe. We analysed data from a large cohort collaboration to characterize HDV epidemiological trends across Europe, as well its impact on clinical outcomes. Methods All PLWH positive B surface antigen (HBsAg) Swiss Cohort Study EuroSIDA between 1988 2019 were tested for anti‐HDV antibodies and, if positive,...
Importance Self- or health care worker (HCW)–collected nasal swab specimens are the preferred sampling method to perform rapid antigen testing for COVID-19, but it is debated whether throat can improve test sensitivity. Objective To compare diagnostic accuracy of self- and HCW-collected vs COVID-19 testing. Design, Setting, Participants This per-protocol multicenter randomized clinical trial was conducted from February 15 through March 25, 2022. The participants, individuals aged 16 years...
Finding the focus of bacterial infections can be challenging, especially for hospitalised patients. Conventional microbiological diagnostic methods are either time consuming, expensive, or difficult to interpret due contamination. The aim this study was apply machine learning (ML) reliably predict infections. This utilised a dataset including samples from 10,153 patients, collected November 1, 2019 June 3, 2023 at Rigshospitalet, Denmark. contains findings, biochemical data, and vital...
People with HIV (PWH) have a high risk of coronary artery disease (CAD). Cytomegalovirus (CMV) co-infection is very common in PWH, but little known about its association CAD. We aimed to investigate if CMV IgG serostatus and concentrations are associated CAD PWH. From the Copenhagen Comorbidity Infection (COCOMO) Study we included PWH CT angiography (CCTA) quantitative concentration measurements. measured stenosis grades plaque volumes vessels. Using multivariable regressions adjusted for...
Respiratory tract infections (RTIs) are a leading cause of morbidity and mortality in children worldwide. Aetiologic virus bacteria vary geographically. To date, no systematic data on RTI aetiologies have been published from Kyrgyzstan, country with high under-five lower RTIs widespread overuse antibiotics. We aimed to identify the Kyrgyzstan assess pneumococcal conjugate vaccine (PCV) vaccination rate correlation detection Streptococcus pneumoniae our population. collected samples aged 6...
Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways patients with cystic fibrosis (CF) promote long-term survival bacteria during chronic lung infection. Twelve clonally related, sequential mucoid non-mucoid paired P. isolates obtained from three Danish CF were investigated. The vitro biofilm formation capacity was studied under static flow through conditions global gene expression profiles investigated by Affymetrix GeneChip. Regulatory genes alginate...
The purpose of the present study was to characterize and compare supragingival salivary microbiotas during a 10-d period oral hygiene discontinuation. We tested hypothesis that composition microbiota will reflect local microbial changes associated with accumulated biofilm formation maturation. Pooled plaque (n = 145) stimulated saliva samples were collected gingival indices recorded from 29 orally healthy individuals at baseline, discontinuation (days 4, 7, 10), 14 d after resumption...