A5051400918- Antibiotic Resistance in Bacteria
- Hepatitis C virus research
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Hepatitis B Virus Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Liver Disease Diagnosis and Treatment
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- Clostridium difficile and Clostridium perfringens research
- Antibiotics Pharmacokinetics and Efficacy
- Immune Cell Function and Interaction
- Escherichia coli research studies
- Enterobacteriaceae and Cronobacter Research
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Urinary Tract Infections Management
- HIV/AIDS Research and Interventions
- SARS-CoV-2 detection and testing
- Nosocomial Infections in ICU
- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Pharmacological Effects of Natural Compounds
Rigshospitalet
2002-2025
Copenhagen University Hospital
2016-2025
University of Copenhagen
2016-2025
Hvidovre Hospital
2013-2024
Amager Hospital
1997-2021
Capital Region of Denmark
1997-2012
Office of Infectious Diseases
1998
Large conjugative plasmids are important drivers of bacterial evolution and contribute significantly to the dissemination antibiotic resistance. Although plasmid borne multidrug resistance is recognized as one main challenges in modern medicine, adaptive forces shaping these within pathogenic hosts poorly understood. Here we study plasmid-host adaptations following transfer a 73 kb naïve clinical isolates Klebsiella pneumoniae Escherichia coli. We use experimental evolution, mathematical...
Extraintestinal pathogenic Escherichia coli (ExPEC) is the main cause of urinary tract infections and septicemia. Significant attention has been given to ExPEC sequence type ST131, which categorized as a “high-risk” clone. High-risk clones are globally distributed associated with various antimicrobial resistance determinants, ease transmission, persistence in hosts, effective transmission between hosts. The high-risk have enhanced pathogenicity severe and/or recurrent infections. We show...
Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to support clinical diagnosis and epidemiological investigations. Recently, large-scale detection of total antibodies (Ab), immunoglobulin G (IgG), IgM against SARS-CoV-2 antigens have been developed, but there limited data on the diagnostic accuracy these assays. This study was a Danish national collaboration evaluated 15 commercial one in-house anti-SARS-CoV-2 in 16 laboratories. Sensitivity...
ObjectivesTo investigate the clinical relevance and molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Klebsiella species in animals.
Mycoplasma genitalium (MG) causes urethritis and cervicitis, potentially causing reproductive complications. Resistance in MG to first-line (azithromycin) second-line (moxifloxacin) treatment has increased. We examined the clinical analytical performance of new Conformité Européene (CE)/in vitro diagnostics (IVD) Aptima assay (CE/IVD AMG; Hologic); prevalence MG, Chlamydia trachomatis (CT) Neisseria gonorrhoeae (NG); resistance azithromycin moxifloxacin Denmark, Norway Sweden 2016.From...
New lineages of SARS-CoV-2 are potential concern due to higher transmissibility, risk severe outcomes, and/or escape from neutralizing antibodies. Lineage B.1.1.7 (the Alpha variant) became dominant in early 2021, but the association between transmissibility and factors, such as age primary case viral load remains poorly understood. Here, we used comprehensive administrative data Denmark, comprising full population (January 11 February 7, 2021), estimate household transmissibility. This...
The ESCPM group (Enterobacter species including Klebsiella aerogenes - formerly Enterobacter aerogenes, Serratia species, Citrobacter freundii complex, Providencia and Morganella morganii) has not yet been incorporated into systematic surveillance programs. We conducted a multicentre retrospective observational study analysing all strains isolated from blood cultures in 27 European hospitals over 3-year period (2020-2022). Diagnostic approach, epidemiology, antimicrobial susceptibility were...
This paper describes how the introduction of "cationic" aliphatic amino groups in chalcone scaffold results potent antibacterial compounds. It is shown that most favorable position for group 2-position B-ring, particular combination with a lipophilic substituent 5-position B-ring. We demonstrate compounds act by unselective disruption cell membranes. Introduction an additional A-ring are selective bacterial membranes combined high activity against both Gram-positive and -negative pathogens....
Abstract Global surveillance for a novel rhinovirus genotype indicated its association with community outbreaks and pediatric respiratory disease in Africa, Asia, Australia, Europe, North America. Molecular dating indicates that these viruses have been circulating at least 250 years.
Elucidating mechanisms leading to the natural control of HIV-1 infection is great importance for vaccine design and understanding viral pathogenesis. Rare HIV-1-infected individuals, termed controllers, have plasma RNA levels below limit detection by standard clinical assays (<50 75 copies/ml) without antiretroviral therapy. Although several recent studies documented persistent low-grade viremia in controllers at a level not significantly different from that individuals undergoing treatment...
CMY-2 is the most common plasmid-mediated AmpC β-lactamase in Escherichia coli isolates of human and animal origin. The aim this study was to elucidate epidemiology CMY-2-producing E. Denmark. Strain plasmid relatedness studied 93 clinical commensal collected from 2006 2012 humans, retail poultry meat, broilers, dogs. Multilocus sequence typing (MLST), antimicrobial susceptibility testing, conjugation were performed conjunction with replicon typing, multilocus (pMLST), restriction fragment...
bla TEM-1 encodes a narrow-spectrum β-lactamase that is inhibited by inhibitors and commonly present in Escherichia coli. Hyperproduction of blaTEM-1 may cause resistance to penicillin/β-lactamase inhibitor (P/BLI) combinations.To characterize EC78, an E. coli bloodstream isolate, resistant P/BLI combinations, which contains extensive amplification within the chromosome.EC78 was sequenced using Illumina Oxford Nanopore Technology (ONT) methodology. Configuration probed PCR. Expression mRNA...
Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has been associated with a high risk of adverse outcomes in solid organ transplant (SOT) recipients the pre-vaccination era. In this retrospective cohort study, we examined incidence and severity COVID-19 kidney liver Denmark post-vaccination era, from December 27, 2020, to 2021. We included 1428 SOT 143 cases first-positive PCR test. The cumulative test 1 year after initiation vaccination was 10.4% (95% CI: 8.8-12.0), higher than...
The emergence of bloodstream infections (BSI) caused by vancomycin-resistant Enterococci (VRE) has concern. Nonetheless, it remains unclear whether these types are associated with an excess risk severe outcomes when compared vancomycin-susceptible (VSE). This cohort study included hospitalized patients in Denmark
Escherichia coli is the most common cause of urinary tract infection (UTI). The pathogenic isolates are becoming increasingly resistant to antibiotics; with a worldwide dissemination sequence types (ST). We characterized three different uropathogenic E. populations, from non-hospitalized patients describe genetic kinship between and susceptible isolates. studied populations by use multi-locus typing (MLST) abbreviated-multi locus variable number tandem repeat analysis (a-MLVA). Urine samples...
We describe clonal shifts in vanA Enterococcus faecium isolates from clinical samples obtained patients Denmark 2015 to the first quarter (Q1) of 2019. During Q1 2019, vancomycin-variable enterococci (VVE) ST1421-CT1134 E. became most dominant clone and has spread all five regions Denmark. Among 174 with vanA, vanB or vanA/vanB genes 44% belonged this type.
For future decisions on respiratory syncytial virus (RSV)-vaccination strategies and implementation into national immunisation-programmes, we used registry data (hospitalisation, microbiology vital statistics) to determine the age-specific incidence direct medical costs of annual RSV-associated admissions in children < 5 years-old for period 2010–2015. We identified ca 2,500 hospitalisations annually amounting total medical-costs EUR 4.1 million per year. The peaked infants 1–2 months age...
Staphylococcus aureus is presently regarded as an emerging zoonotic agent due to the spread of specific methicillin-resistant S. (MRSA) clones in pig farms. Studying microbiota can be useful for identification bacteria that antagonize such opportunistic veterinary and pathogen animal carriers. The aim this study was determine whether nasal microbiome carriers differs from non-carriers. V3-V5 region 16S rRNA gene sequenced swabs 44 56 non-carriers using 454 GS FLX titanium system. Carriers...
Summary Background A defined bacterial mixture could be a safer alternative to faecal microbiota transplantation (FMT). Aims To compare the efficacy of 12‐strain termed rectal bacteriotherapy with either FMT or vancomycin for recurrent Clostridioides difficile infection (CDI) in an open‐label 3‐arm randomised controlled trial. Methods We screened all individuals positive C from May 2017 March 2019. Persons laboratory‐confirmed CDI were included. Before and bacteriotherapy, we pre‐treated...
Abstract Background In early 2021, the SARS-CoV-2 lineage B.1.1.7 (Alpha variant) became dominant across large parts of world. Denmark, comprehensive and real-time test, contact-tracing, sequencing efforts were applied to sustain epidemic control. Here, we use these data investigate transmissibility, introduction, onward transmission in Denmark. Methods We analyzed a set 60,178 genomes generated from high-throughput by Danish COVID-19 Genome Consortium, representing 34% all positive cases...