A5033991931- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- COVID-19 epidemiological studies
- Cellular Mechanics and Interactions
- Escherichia coli research studies
- Antifungal resistance and susceptibility
- Global Maternal and Child Health
- Respiratory viral infections research
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- Child Nutrition and Water Access
- Forensic Anthropology and Bioarchaeology Studies
- Mycobacterium research and diagnosis
- Reproductive tract infections research
- Parasitic Infections and Diagnostics
- Fungal Infections and Studies
- Forensic Entomology and Diptera Studies
- Protist diversity and phylogeny
University of Sassari
2015-2024
Azienda Ospedaliero Universitaria di Sassari
2015-2024
University of Genoa
2022
University of Belgrade
2022
University of Washington
2018-2020
Institute for Health Metrics and Evaluation
2018-2020
Sapienza University of Rome
2019
King Faisal Specialist Hospital & Research Centre
2014-2018
All India Institute of Medical Sciences
2018
Biology of Infection
2011-2017
We have developed a simple and efficient procedure for adding an epitope-encoding tail to one or more genes of interest in the bacterial chromosome. The is modification gene replacement method Datsenko Wanner [Datsenko, K. A. & Wanner, B. L. (2000) Proc. Natl. Acad. Sci. USA 97, 6640–6645]. A DNA module that begins with sequence includes selectable marker amplified by PCR primers carry extensions (as short as 36 nt) homologous last portion targeted region downstream from it....
As of 28 February 2020, Italy had 888 cases SARS-CoV-2 infections, with most in Northern the Lombardia and Veneto regions. Travel-related were main source COVID-19 during early stages current epidemic Italy. The month February, however, has been dominated by two large clusters outbreaks Italy, south Milan, mainly local transmission infections. Contact tracing failed to identify patient zero one outbreaks. twenty-one have died. Comparison between case fatality rates China are identical at...
18 years ago, in 2002, the world was astonished by appearance of Severe Acute Respiratory Syndrome (SARS), supported a zoonotic coronavirus, called SARS-CoV, from Guangdong Province southern China. After about 10 years, 2012, another similar coronavirus triggered Middle East (MERS-CoV) Saudi Arabia. Both caused severe pneumonia killing 774 and 858 people with 8700 cases confirmed infection for former, 2494 latter, causing significant economic losses. 8 later, despite MERS outbreak remaining...
On 31 December 2019 the Wuhan Health Commission reported a cluster of atypical pneumonia cases that was linked to wet market in city Wuhan, China. The first patients began experiencing symptoms illness mid-December 2019. Clinical isolates were found contain novel coronavirus with similarity bat coronaviruses. As 28 January 2020, there are excess 4,500 laboratory-confirmed cases, > 100 known deaths. SARS-CoV, infections children appear be rare. Travel-related have been confirmed multiple...
Abstract Severe acute respiratory syndrome coronavirus 2 infection might induce a significant and sustained lymphopenia, increasing the risk of developing opportunistic infections. Mucormycosis is rare but severe invasive fungal infection, mainly described in immunocompromised patients. The first case patient diagnosed with disease (COVID-19) who developed pulmonary mucormycosis extensive cavitary lesions here reported. This highlights how this new impair immune response, exposing patients...
Sardinia, Italy, has a high prevalence of residents who live more than 100 years. The reasons for longevity in this isolated region are currently unknown. Gut microbiota may hold clue. To explore the role gut play healthy aging and longevity, we used metagenomic sequencing to determine compositional functional differences associated with populations different ages Sardinia. Our data revealed that both young elderly Sardinians shared similar taxonomic profiles. A pattern was found...
ABSTRACT Human infections with nontyphoidal Salmonella serotypes, such as S. enterica serotype Typhimurium, are characterized by a massive neutrophil influx in the colon and terminal ileum. In contrast, neutrophils scarce intestinal infiltrates of typhoid fever patients. Here, we show that Typhi, causative agent fever, expression Vi capsular antigen reduced chemoattractant interleukin-8 (IL-8) host cells. Capsulated bacteria elicited IL-8 polarized human epithelial cells (T84)...
The Chikungunya virus infection zones have now quickly spread from Africa to parts of Asia, North America and Europe. Originally thought trigger a disease only mild symptoms, recently caused large-scale fatalities widespread economic loss that was linked recent genetic mutation evolution. Due the paucity information on immunological progression, we investigated serum levels 13 cytokines/chemokines during acute phase 6- 12-month post-infection follow-up patients Italian outbreak. We found...
Pandemic H1N1 influenza A (H1N1pdm) is currently a dominant circulating strain worldwide. Severe cases of H1N1pdm infection are characterized by prolonged activation the immune response, yet specific role inflammatory mediators in disease poorly understood. The cytokine IL-6 has been implicated both seasonal and severe pandemic infection. Here, we investigated We found to be an important feature host response humans mice infected with H1N1pdm. Elevated levels were associated patients...
The island of Sardinia has been particular interest to geneticists for decades. current model Sardinia's genetic history describes the as harboring a founder population that was established largely from Neolithic peoples southern Europe and remained isolated later Bronze Age expansions on mainland. To evaluate this model, we generate genome-wide ancient DNA data 70 individuals 21 Sardinian archaeological sites spanning Middle through Medieval period. earliest show strong affinity western...
COVID-19 case fatalities surged during the month of March 2020 in Italy, reaching over 10,000 by 28 2020. This number exceeds China (3,301) recorded from January to March, even though diagnosed cases was similar (85,000 Italy vs. 80,000 China). Case Fatality Rates (CFR) could be somewhat unreliable because estimation total numbers is limited several factors, including insufficient testing and limitations test kits materials, such as NP swabs PPE for testers. Sero prevalence SARS-CoV-2...
Drug resistance in antifungal therapy, a problem unknown until few years ago, is increasingly assuming importance especially immunosuppressed patients and receiving chemotherapy radiotherapy. In the past years, use of essential oils as an approach to improve effectiveness agents reduce levels has been proposed. Our research aimed evaluate activity Colombian rue, Ruta graveolens, oil (REO) against clinical strains Candida albicans, parapsilopsis, glabrata, tropicalis. Data obtained showed...
The ESCPM group (Enterobacter species including Klebsiella aerogenes - formerly Enterobacter aerogenes, Serratia species, Citrobacter freundii complex, Providencia and Morganella morganii) has not yet been incorporated into systematic surveillance programs. We conducted a multicentre retrospective observational study analysing all strains isolated from blood cultures in 27 European hospitals over 3-year period (2020-2022). Diagnostic approach, epidemiology, antimicrobial susceptibility were...
Malaria-causing protozoa of the genus Plasmodium have exerted one strongest selective pressures on human genome, and resistance alleles provide biomolecular footprints that outline historical reach these species
Background: Salmonellosis remains one of the most frequent food-borne diseases worldwide, especially in developing countries. The emergence antimicrobial resistance Salmonella isolates from food can potentially compromise treatment these infections. This investigation was conducted for first time Morocco both to detect occurrence foods as well determine antibiotic profile isolates. Methodology: In total, 11,516 samples collected 2002 2005 were investigated. Isolated characterized by...
To define the genetic characteristics and resistance mechanisms of clinical isolates Salmonella enterica serovar Typhi (S. Typhi) S. Paratyphi A A) exhibiting high-level fluoroquinolones resistance.Three two ciprofloxacin-resistant (MICs > 4 mg/L) were compared with reduced susceptibility to ciprofloxacin 0.125-1 by PFGE, plasmid analysis, presence integrons nucleotide changes in topoisomerase genes.In A, a single gyrA mutation (Ser-83-->Phe or Ser-83-->Tyr) was associated mg/L); an...
Synergistic combinations of antimicrobial agents with different mechanisms action have been introduced as more successful strategies to combat infections involving multidrug resistant (MDR) bacteria. In this study, we investigated synergistic activity Camellia sinensis and Juglans regia which are commonly used plants agents. Antimicrobial susceptibility 350 Gram-positive Gram-negative strains belonging 10 bacterial species, was tested against extracts. Minimum inhibitory concentrations...
Through a comprehensive analysis of Italy's estimates from the Global Burden Diseases, Injuries, and Risk Factors Study (GBD) 2017, we aimed to understand patterns health loss response health-care system, offer evidence-based policy indications in light demographic transition government spending country.
Sardinians are "outliers" in the European genetic landscape and, according to paleogenomic nuclear data, closest early Neolithic farmers. To learn more about their ancestry, we analyzed 3,491 modern and 21 ancient mitogenomes from Sardinia. We observed that 78.4% of cluster into 89 haplogroups most likely arose situ. For each Sardinian-specific haplogroup (SSH), also identified upstream node phylogeny, which non-Sardinian radiate. This provided minimum maximum time estimates for presence SSH...
Staphylococcus epidermidis and aureus are currently considered two of the most important pathogens in nosocomial infections associated with catheters other medical implants also main contaminants instruments. However because these species part normal bacterial flora human skin mucosal surfaces, it is difficult to discern when a microbial isolate cause infection or detected on samples as consequence contamination. Rapid identification invasive strains crucial for correctly diagnosing treating...