A5041967571- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- COVID-19 epidemiological studies
- Virus-based gene therapy research
- Hepatitis B Virus Studies
- Vaccine Coverage and Hesitancy
- Long-Term Effects of COVID-19
- Disaster Response and Management
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- CRISPR and Genetic Engineering
- Immune responses and vaccinations
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Poxvirus research and outbreaks
- Global Security and Public Health
- Phenothiazines and Benzothiazines Synthesis and Activities
University of Saskatchewan
2016-2025
Canadian Science Centre for Human and Animal Health
2023
Institut de Recherche Vaccinale
2023
Vancouver Infectious Diseases Centre
2020-2022
National Institute of Allergy and Infectious Diseases
2010-2018
National Institutes of Health
2010-2018
State Key Laboratory of Virology
2013-2016
Friedrich-Loeffler-Institut
2015
Liberian Institute for Biomedical Research
2015
Centers for Disease Control and Prevention
2015
In 2012, a novel betacoronavirus, designated Middle East respiratory syndrome coronavirus or MERS-CoV and associated with severe disease in humans, emerged the Arabian Peninsula. To date, 108 human cases have been reported, including of human-to-human transmission. The availability an animal model is essential for understanding pathogenesis developing effective countermeasures. Upon combination intratracheal, ocular, oral, intranasal inoculation 7 × 10(6) 50% tissue culture infectious dose...
The identification of a novel β coronavirus, nCoV, as the causative agent severe respiratory illness in humans originating Saudi Arabia, Qatar and Jordan has raised concerns about possibility coronavirus pandemic similar to that SARS-CoV. As definitive treatment regimen never been thoroughly evaluated for infections, there is an urgent need rapidly identify potential therapeutics address future cases nCoV. To determine intervention strategy, effect interferon-α2b ribavirin on nCoV isolate...
A consensus MERS spike protein synthetic DNA vaccine can induce protective responses against viral challenge.
On 31 December 2019 the Wuhan Health Commission reported a cluster of atypical pneumonia cases that was linked to wet market in city Wuhan, China. The first patients began experiencing symptoms illness mid-December 2019. Clinical isolates were found contain novel coronavirus with similarity bat coronaviruses. As 28 January 2020, there are excess 4,500 laboratory-confirmed cases, > 100 known deaths. SARS-CoV, infections children appear be rare. Travel-related have been confirmed multiple...
The availability of a robust disease model is essential for the development countermeasures Middle East respiratory syndrome coronavirus (MERS-CoV). While rhesus macaque MERS-CoV has been established, lack uniform, severe in this complicates analysis countermeasure studies. Modeling interaction between spike glycoprotein and its receptor dipeptidyl peptidase 4 predicted comparable energies common marmosets humans. suitability marmoset as was tested by inoculation via combined intratracheal,...
Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at field diagnostic laboratory Liberia the current Ebola virus outbreak.
Ebola virus (EBOV), an enveloped, single-stranded, negative-sense RNA virus, causes severe hemorrhagic fever in humans and nonhuman primates. The EBOV glycoprotein (GP) gene encodes the nonstructural soluble (sGP) but also produces transmembrane (GP₁,₂) through transcriptional editing. A third GP product, a small (ssGP), has long been postulated to be produced as result of To identify characterize expression this new protein, we first analyzed relative ratio gene-derived transcripts during...
(See the editorial commentary by Bausch, on pages 179-81.)Reston ebolavirus was recently detected in pigs Philippines. Specific antibodies were found pig farmers, indicating exposure to virus. This important observation raises possibility that may be susceptible Ebola virus infection, including from other species, such as Zaire (ZEBOV), and can transmit hosts.This study investigated whether ZEBOV, a species commonly reemerging central Africa, replicate induce disease transmitted naive...
Ebola hemorrhagic fever (EHF) is a severe viral infection for which no effective treatment or vaccine currently available. While the nonhuman primate (NHP) model used final evaluation of experimental vaccines and therapeutic efficacy, rodent models have been widely in ebolavirus research because their convenience. However, validity has questioned given low predictive value efficacy testing therapeutics, result inconsistent manifestation coagulopathy seen EHF. Here, we describe lethal Syrian...
Significance Effective therapies are urgently needed for COVID-19. We rapidly (within a week) identified fully human monoclonal germline-like antibody (ab1) from phage-displayed libraries that potently inhibited mouse ACE2-adapted SARS-CoV-2 replication in wild-type BALB/c mice and native virus transgenic expressing ACE2 as well hamsters when administered before challenge. It was also effective after infection of hamsters, although at lower efficacy than used prophylactically. Ab1 highly...
COVID-19 (coronavirus disease 2019) caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) infection is a affecting several organ systems. A model that captures all clinical symptoms of as well long-haulers needed. We investigated the host responses associated with in major systems including tract, heart, and kidneys after Syrian hamsters. found significant increases inflammatory cytokines (IL-6, IL-1beta, TNF) type II interferons whereas I were inhibited. Examination...
Significance Type I interferons (IFN-I) exhibit various biological effects during viral infections, and they have been successfully used for clinical treatment of diseases. Humans express 12 IFNα subtypes, which strongly differ in their antiviral responses against different viruses. Here we analyzed the activity all human subtypes severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to identify underlying immune signatures explore therapeutic potential. Our data provide a systemic...
Abstract Post-acute sequelae of COVID-19 (PASC) or the continuation (Coronavirus disease 2019) symptoms past 12 weeks may affect as many 30% people recovering from a SARS-CoV-2 (severe acute respiratory coronavirus 2) infection. The mechanisms regulating development PASC are currently not known; however, hypotheses include virus reservoirs, pre-existing conditions, microblood clots, immune dysregulation, well poor antibody responses. Importantly, neutralizing antibodies essential for...
Currently, there is no registered treatment for infections with emerging zoonotic coronaviruses like SARS- and MERS-coronavirus. We here report that in cultured cells low-micromolar concentrations of alisporivir, a non-immunosuppressive cyclosporin A-analog, inhibit the replication four different coronaviruses, including MERS- SARS-coronavirus. Ribavirin was found to further potentiate antiviral effect alisporivir these cell culture-based infection models, but this combination unable improve...
ABSTRACT Hantavirus pulmonary syndrome (HPS) is caused by infection with several Sigmodontinae - and Neotominae -borne hantaviruses has a case fatality rate of 30 to 50%. Humans often become infected inhalation materials contaminated virus-laden rodent urine or saliva, although human-to-human transmission also been documented for Andes virus (ANDV). The ability transmit via aerosolization, coupled the high mortality rates lack therapeutic options, makes development medical countermeasures...
The emerging coronavirus disease 2019 (COVID-19) outbreak caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has rapidly spread worldwide, resulting in global public health emergencies and economic crises. In the present study, a noninfectious biosafety level (BSL2)-compatible SARS-CoV-2 replicon expressing nano luciferase (nLuc) reporter was constructed bacterial artificial chromosomal (BAC) vector reverse genetics. nLuc is highly sensitive, easily quantifiable, high throughput...