A5054316522- Methane Hydrates and Related Phenomena
- SARS-CoV-2 and COVID-19 Research
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Earthquake Detection and Analysis
- Seismic Waves and Analysis
- Crystallization and Solubility Studies
- Parasite Biology and Host Interactions
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- X-ray Diffraction in Crystallography
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- Photodynamic Therapy Research Studies
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Nanoparticle-Based Drug Delivery
- Microbial infections and disease research
- SARS-CoV-2 detection and testing
- Advanced Electron Microscopy Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Parasitic Infections and Diagnostics
- COVID-19 Clinical Research Studies
- Photoacoustic and Ultrasonic Imaging
Daping Hospital
2025
Army Medical University
2023-2025
University of British Columbia
2020-2024
Guizhou University
2021-2024
Sun Yat-sen University
2020-2023
Shandong University
2023
Army Medical College
2023
Capital Medical University
2018-2022
Beijing Children’s Hospital
2021-2022
National Center for Women and Children’s Health, China CDC
2022
The newly reported Omicron variant is poised to replace Delta as the most prevalent SARS-CoV-2 across world. Cryo-EM structural analysis of spike protein in complex with human ACE2 reveals new salt bridges and hydrogen bonds formed by mutated residues R493, S496 R498 RBD ACE2. These interactions appear compensate for other mutations such K417N known reduce binding affinity, resulting similar biochemical affinities variants. Neutralization assays show that pseudoviruses displaying exhibit...
The recently reported "UK variant" (B.1.1.7) of SARS-CoV-2 is thought to be more infectious than previously circulating strains as a result several changes, including the N501Y mutation. We present 2.9-Å resolution cryo-electron microscopy (cryo-EM) structure complex between ACE2 receptor and spike protein ectodomains that shows Y501 inserted into cavity at binding interface near Y41 ACE2. This additional interaction provides structural explanation for increased affinity mutant, likely...
The Delta and Kappa variants of SARS-CoV-2 co-emerged in India late 2020, with the variant underlying resurgence COVID-19, even countries high vaccination rates. In this study, we assess structural biochemical aspects viral fitness for these two using cryo-electron microscopy (cryo-EM), ACE2-binding antibody neutralization analyses. Both demonstrate escape antibodies targeting N-terminal domain, an important immune hotspot neutralizing epitopes. Compared to wild-type lineages, spike proteins...
With unknown etiology and limited treatment options, neonatal hypoxic-ischemic brain damage (HIBD) remains a major cause of mortality in newborns. Ferroptosis, recently discovered type cell death triggered by lipid peroxidation, is closely associated with HIBD. High-mobility group box 1 (HMGB1), molecule inflammation damage, can induce neuronal However, it whether HMGB1 contributes to ferroptosis patients Herein, glycyrrhizin (GL), an inhibitor, was used investigate the relationship between...
Connexins, like true cell adhesion molecules, have extracellular domains that provide strong and specific homophilic, in some cases, heterophilic interactions between cells. Though the structure of binding proteins been determined, connexins, consisting two loops ∼34–37 amino acids each, are not easily studied isolation from rest molecule. As an alternative, we used a novel application site-directed mutagenesis which four six conserved cysteines connexin 32 were moved individually all...
Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete is a gigantic complex with molecular mass of approximately five megadaltons. Here, we report the nearly architecture Saccharomyces cerevisiae determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. majority density maps were modeled and assigned specific RNA protein components. nascent ribosome isolated native-like substructures that...
The recently emerged severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Beta (B.1.351) and Gamma (P.1) variants of concern (VoCs) include a key mutation (N501Y) found in the Alpha (B.1.1.7) variant that enhances affinity spike protein for its receptor, angiotensin-converting enzyme 2 (ACE2). Additional mutations are these at residues 417 484 appear to promote antibody evasion. In contrast, Epsilon (B.1.427/429) lack N501Y yet exhibit We have engineered proteins express receptor...
Biomolecular condensates have been implicated in multiple cellular processes. However, the global role played by 3D chromatin organization remains unclear. At present, 1,6-hexanediol (1,6-HD) is only available tool to globally disrupt condensates, yet conditions of 1,6-HD vary considerably between studies and may even trigger apoptosis.In this study, we first analyzed effects different concentrations treatment durations found that short-term exposure 1.5% dissolved biomolecular whereas...
Abstract Mutations in the spike glycoproteins of SARS-CoV-2 variants concern have independently been shown to enhance aspects protein fitness. Here, we describe an antibody fragment (V H ab6) that neutralizes all major including recently emerged BA.1 and BA.2 Omicron subvariants, with a unique mode binding revealed by cryo-EM studies. Further, provide comparative analysis mutational effects within previously variant spikes identify structural role mutations NTD RBD evading neutralization....
Abstract The XBB.1.5 variant of SARS-CoV-2 has rapidly achieved global dominance and exhibits a high growth advantage over previous variants. Preliminary reports suggest that the success stems from mutations within its spike glycoprotein, causing immune evasion enhanced receptor binding. We present binding studies demonstrate retention contacts with human ACE2 striking decrease in to mouse due revertant R493Q mutation. Despite extensive antibody binding, we highlight region on protein domain...
Assembly of eukaryotic ribosome is a complicated and dynamic process that involves series intermediates. It unknown how the highly intertwined structure 60S large ribosomal subunits established. Here, we report an early nucleolar pre-60S determined by cryo-electron microscopy at 3.7 Å resolution, revealing half-assembled subunit. Domains I, II VI 25S/5.8S rRNA pack tightly into native-like substructure, but domains III, IV V are not assembled. The contains 12 assembly factors 19 proteins,...
How ribosomes are made The formation of eukaryotic is a complex process that starts with transcription large precursor RNA assembles into 90S preribosome, which matures to finally give the 40S small subunit ribosome. Cheng et al. and Du insight this process, using cryo–electron microscopy look at intermediates along pathway. Together, these studies reveal how cast molecular players act coordinate compositional structural changes transform preribosome pre-40S subunit. Science , issue p. 1470 1477
This ‘nanobomb’ can mechanically destroy tumor vessels, significantly relieve hypoxia within the and reduce microvessel density.
Abstract Chemical cross-linking of proteins coupled with mass spectrometry analysis (CXMS) is widely used to study protein-protein interactions (PPI), protein structures, and even dynamics. However, structural information provided by CXMS still limited, partly because most experiments use lysine-lysine (K-K) cross-linkers. Although superb in selectivity reactivity, they are ineffective for lysine deficient regions. Herein, we develop aromatic glyoxal cross-linkers (ArGOs) arginine-arginine...
The newly reported Omicron variant is poised to replace Delta as the most rapidly spread SARS-CoV-2 across world. Cryo-EM structural analysis of spike protein in complex with human ACE2 reveals new salt bridges and hydrogen bonds formed by mutated residues R493, S496 R498 RBD ACE2. These interactions appear compensate for other mutations such K417N known reduce binding affinity, explaining our finding similar biochemical affinities variants. Neutralization assays show that pseudoviruses...
Curcumin is extracted from the rhizomes of traditional Chinese herb Curcuma longa. Our previous study indicated curcumin was able to function as a sonosensitizer. Hydroxyl acylated synthesized eliminate unstable hydroxy perssad in our group. The potential use sonosensitizer for sonodynamic therapy (SDT) requires further exploration. This investigated effect on THP-1 macrophage. macrophages were cultured with at concentration 5.0 μg/mL 4 hours and then exposed pulse ultrasound irradiation...
Abstract: Atherosclerosis (AS) is the most vital cardiovascular disease, which poses a great threat to human health. Macrophages play an important role in progression of AS. Photodynamic therapy (PDT) has emerged as useful therapeutic modality not only treatment cancer but also The purpose this study was determine molecular mechanisms underlying activity PDT, using mesoporous-silica-coated upconversion fluorescent nanoparticles encapsulating chlorin e6 (UCNPs-Ce6) induction apoptosis THP-1...
The BA.2 sub-lineage of the Omicron (B.1.1.529) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant rapidly supplanted original BA.1 in early 2022. Both lineages threatened efficacy vaccine-elicited antibodies and acquired increased binding to several mammalian ACE2 receptors. Cryoelectron microscopy (cryo-EM) analysis spike (S) glycoprotein complex with mouse (mACE2) identifies BA.1- BA.2-mutated residues Q493R, N501Y, Y505H as complementing non-conserved between human...