A5061989599- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Microscopic Colitis
- COVID-19 Clinical Research Studies
- Adipose Tissue and Metabolism
- Gut microbiota and health
- Genetic Associations and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Diabetes and associated disorders
- Immunotherapy and Immune Responses
- Inflammatory Bowel Disease
- IL-33, ST2, and ILC Pathways
- Single-cell and spatial transcriptomics
- Cardiovascular Effects of Exercise
- Parasites and Host Interactions
- Long-Term Effects of COVID-19
- Cardiac pacing and defibrillation studies
- Cardiac Imaging and Diagnostics
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- Atrial Fibrillation Management and Outcomes
- Vitamin D Research Studies
- Cardiac Arrhythmias and Treatments
- HIV Research and Treatment
University of British Columbia
2016-2025
The University of Melbourne
2021-2025
Peter Doherty Institute
2021-2025
British Columbia Children's Hospital
2016-2024
St Vincent's Hospital Sydney
2017-2022
UNSW Sydney
2014-2022
Norfolk and Norwich University Hospitals NHS Foundation Trust
2022
Norfolk and Norwich University Hospital
2021
Child and Family Research Institute
2016
National Jewish Health
2005-2008
These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed....
Cardiac involvement among hospitalized patients with severe coronavirus disease 2019 (COVID-19) is common and associated adverse outcomes. This study aimed to determine the prevalence clinical implications of COVID-19 cardiac in young competitive athletes.In this prospective, multicenter, observational cohort data from 42 colleges universities, we assessed prevalence, characteristics, outcomes collegiate athletes United States. Data were collected September 1, 2020, December 31, 2020. The...
Immunotherapy targeting the autoimmune process in type 1 diabetes (T1D) can delay loss of β-cells but needs to have minimal adverse effects be an adjunct insulin management T1D. Ustekinumab binds shared p40 subunit interleukin (IL)-12 and IL-23, development T helper cells 17 (T
Abstract The XBB.1.5 variant of SARS-CoV-2 has rapidly achieved global dominance and exhibits a high growth advantage over previous variants. Preliminary reports suggest that the success stems from mutations within its spike glycoprotein, causing immune evasion enhanced receptor binding. We present binding studies demonstrate retention contacts with human ACE2 striking decrease in to mouse due revertant R493Q mutation. Despite extensive antibody binding, we highlight region on protein domain...
Celiac disease is a chronic immune-mediated inflammatory disorder of the gut triggered by dietary gluten. Although effector T-cell response in patients with celiac has been well characterized, role regulatory T (Treg) cells loss tolerance to gluten remains poorly understood.We sought define whether have dysfunction or lack gluten-specific forkhead box protein 3 (FOXP3)+ Treg cells.Treated underwent oral wheat challenge stimulate recirculation cells. Peripheral blood was collected before and...
In the initial stages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) COVID-19 pandemic, a plethora new serology tests were developed and introduced to global market. Many not evaluated rigorously, there is significant lack concordance in results across methods. To enable meaningful clinical decisions be made, robustly evaluated, quantitative methods are needed. These should harmonized primary reference material, allowing for comparison trial data improved decision making. A...
Pulmonary gammadelta T cells protect the lung and its functions, but little is known about their distribution in this organ relationship to other pulmonary cells. We now show that alphabeta are distributed differently normal mouse lung. The have a bias for nonalveolar locations, with exception of airway mucosa. Subsets exhibit further variation tissue localization. frequently contact leukocytes, they favor different cell-types. an intrinsic preference F4/80+ major histocompatibility complex...
Human Ag‐specific CD4 + T cells can be detected by their dual expression of CD134 (OX40) and CD25 after a 44 hours stimulation with cognate Ag. We show that surface CD39 on consistently identifies substantial population have Treg‐cell‐like phenotype mostly originate from bulk memory CD45RO CD127 low high Treg cells. Viable, could expanded in vitro as cell lines clones, retained Forkhead Box Protein 3, CTLA‐4 expression, suppressive activity Ag specificity. also utilised this combination...
In patients with Wolff‐Parkinson‐White syndrome (WPW), it is important to assess the ventricular response during atrial flutter or fibrillation since conduction across accessory pathway these rhythms may cause hemodynamic impairment life‐threatening arrhythmias. We have recently reported effective use of an esophageal electrode in pacing atrium. this study we praspectively assessed ability induce and by 23 WPW other electrophysiological abnormalities. An bipolar 29 mm interelectrode distance...
The prevalence of childhood obesity is increasing worldwide, along with the associated common comorbidities type 2 diabetes and cardiovascular disease in later life. Motivated by evidence for a strong genetic component, our prior genome-wide association study (GWAS) efforts revealed 19 independent signals trait; however, mechanism action these loci remains to be elucidated. To molecularly characterize loci, we sought determine underlying causal variants corresponding effector genes within...
Abstract Background Inflammatory bowel disease (IBD) affects an estimated 270,000 people in Canada and is increasing prevalence. Current treatments, such as anti-TNFα mAbs, block inflammatory pathways, but require repeated dosing do not reverse intestinal fibrosis. Additionally, a significant subset of IBD patients have relapsing respond to biologics. We shown type 1 regulatory cells (Tr1s) are capable suppressing inflammation via IL-10, promote gut-healing barrier function IL-22,...
Background & AimsBacterial flagellin is an important antigen in inflammatory bowel disease, but the role of flagellin-specific CD4+ T cells disease pathogenesis remains unclear. Also unknown how changes intestinal microbiome intersect with those microbiota-specific cells. We aimed to quantify and characterize Crohn's (CD) ulcerative colitis (UC) patients study their relationship diversity.MethodsBlood was collected from 3 cohorts that included CD patients, UC healthy controls. Flow cytometry...
Immune homeostasis in the intestine is tightly controlled by FOXP3+ regulatory T cells (Tregs), defects of which are linked to development chronic conditions, such as inflammatory bowel disease (IBD). As a mechanism immune evasion, several species intestinal parasites boost Treg activity. The parasite Heligmosomoides polygyrus known secrete molecule (Hp-TGM) that mimics ability TGF-β induce FOXP3 expression CD4+ cells. study aimed investigate whether Hp-TGM could human Tregs potential...