A5016532166- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Herpesvirus Infections and Treatments
- Long-Term Effects of COVID-19
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Cytomegalovirus and herpesvirus research
- Infection Control and Ventilation
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Liver Disease Diagnosis and Treatment
- Dental Research and COVID-19
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- interferon and immune responses
- COVID-19 Impact on Reproduction
- COVID-19 and healthcare impacts
- Hepatitis Viruses Studies and Epidemiology
- COVID-19 and Mental Health
- Poxvirus research and outbreaks
- COVID-19 epidemiological studies
- Tryptophan and brain disorders
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
Essen University Hospital
2013-2024
University of Duisburg-Essen
2015-2024
National Center for Tumor Diseases
2010-2013
Heidelberg University
2010
Ruhr University Bochum
2009
Abstract Long-term antibody responses and neutralizing activities in response to SARS-CoV-2 infection are not yet clear. Here we quantify immunoglobulin M (IgM) G (IgG) antibodies recognizing the receptor-binding domain (RBD) of spike (S) or nucleocapsid (N) protein, during a period 6 months from COVID-19 disease onset 349 symptomatic patients who were among first be infected world-wide. The positivity rate magnitude IgM-S IgG-N increase rapidly. High levels IgM-S/N IgG-S/N at 2-3 weeks...
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic creates a significant threat to global health. Recent studies suggested the significance of throat and salivary glands as major sites virus replication transmission during early disease 2019, thus advocating application oral antiseptics. However, antiviral efficacy rinsing solutions against SARS-CoV-2 has not been examined. Here, we evaluated virucidal activity different available rinses under conditions...
The outbreak of SARS-CoV-2 developed into a global pandemic affecting millions people worldwide. Despite one year intensive research, the current treatment options for infected are still limited. Clearly, novel antiviral compounds patients urgently needed. Complementary medicine is used along with standard medical and accessible to vast majority Natural products activity may contribute improve overall condition individuals. In present study, we investigated glycyrrhizin, primary active...
Abstract We investigated immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among a group of convalescent, potential blood donors in Germany who had PCR-confirmed SARS-CoV-2 infection. Sixty days after onset symptoms, 13/78 (17%) study participants borderline or negative results to an ELISA detecting IgG the S1 protein SARS-CoV-2. analyzed with infection strong antibody (ratio >3) as positive controls and without symptoms household contact infected patients...
Abstract We investigated immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among a group of convalescent, potential blood donors in Germany who had PCR-confirmed SARS-CoV-2 infection. Sixty days after onset symptoms, 13/78 (17%) study participants borderline or negative results to an ELISA detecting IgG the S1 protein SARS-CoV-2. analyzed with infection strong antibody (ratio >3) as positive controls and without symptoms household contact infected patients...
Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2, a new member of the genus Betacoronavirus, is pandemic virus, which has caused numerous fatalities, particularly in elderly and persons with underlying morbidities. At present, there are no approved vaccines nor antiviral therapies available. The detection quantification SARS-CoV-2-neutralizing antibodies plays crucial role assessment immune status convalescent COVID-19 patients, evaluation recombinant therapeutic antibodies,...
Finite therapy of HBV/HDV co-infection with REP 2139-Ca and pegIFN has good long term safety is accompanied by high rates functional cure HDV, normalization liver function additional HBV. HBV inactivation / clearance cccDNA integrated DNA. The nucleic acid polymer 2139 inhibits assembly/secretion hepatitis B virus (HBV) subviral particles. Previously, pegylated interferon (pegIFN) in HBV/hepatitis delta (HDV) coinfection achieved HDV RNA surface antigen (HBsAg) loss/seroconversion the 301...
Severe Acute Respiratory Syndrome Coronavirus Type 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). The availability effective and well-tolerated antiviral drugs for treatment COVID-19 patients still very limited. Traditional herbal medicines elicit activity against various viruses might therefore represent a promising option complementary patients. application turmeric root in medicine has long history. Its bioactive ingredient curcumin shows broad-spectrum...
Significance Type I interferons (IFN-I) exhibit various biological effects during viral infections, and they have been successfully used for clinical treatment of diseases. Humans express 12 IFNα subtypes, which strongly differ in their antiviral responses against different viruses. Here we analyzed the activity all human subtypes severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to identify underlying immune signatures explore therapeutic potential. Our data provide a systemic...
Despite the availability of antiviral chemotherapy, herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) infections remain a severe global health problem. Of particular concern is growing incidence drug resistance in immunocompromised patients, which stresses urgency to develop new effective treatment alternatives. We have developed humanized monoclonal antibody (mAb hu2c) that completely abrogates viral cell-to-cell spread, key mechanism by HSV-1/2 escapes humoral immune surveillance....
Abstract Long-term antibody responses and neutralizing activities following SARS-CoV-2 infections have not yet been elucidated. We quantified immunoglobulin M (IgM) G (IgG) antibodies recognizing the receptor-binding domain (RBD) of spike (S) or nucleocapsid (N) protein, during a period six months COVID-19 disease onset in 349 symptomatic patients, which were among first world-wide being infected. The positivity rate magnitude IgM-S IgG-N increased rapidly. High levels IgM-S/N IgG-S/N at 2-3...
The outbreak of the SARS-CoV-2 pandemic is triggering a global health emergency alert. Until vaccination becomes available, bundle effective preventive measures desperately needed. Recent research indicating relevance aerosols in spread SARS-CoV-2. Thus, this study commercially available antiseptic mouthwashes based on active ingredients chlorhexidine digluconate and octenidine dihydrochloride (OCT) were investigated regarding their efficacy against using European Standard 14476. Based...
Hepatitis D virus (HDV) superinfection in patients with hepatitis B (HBV) is associated rapid progression to liver cirrhosis and hepatocellular carcinoma. Treatment options are limited, no vaccine available. Although HDV-specific CD8+ T cells thought control the virus, little known about which HDV epitopes targeted by virus-specific or why these ultimately fail infection. We aimed define how escapes T-cell-mediated response.We collected plasma DNA samples from 104 chronic HBV infection at...
The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome 2 (SARS-CoV-2) is currently most pressing medical and socioeconomic challenge. Constituting important correlates of protection, determination virus-neutralizing antibodies (NAbs) indispensable for convalescent plasma selection, vaccine candidate evaluation, immunity certificates. In contrast to standard serological ELISAs, plaque reduction neutralization tests (PRNTs) are laborious, time-consuming,...
The role of adaptive SARS-CoV-2 specific immunity in post-acute sequelae COVID-19 (PASC) is not well explored, although a growing population convalescent patients with manifestation PASC observed. We analyzed the SARS-CoV-2-specific immune response, via pseudovirus neutralizing assay and multiparametric flow cytometry 40 non-specific 15 healthy donors. Although frequencies SARS-CoV-2-reactive CD4+ T cells were similar between studied cohorts, stronger reactive CD8+ cell characterized by IFNγ...
Background Breakthrough infections with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are increasingly observed in vaccinated individuals. Immune responses towards SARS-CoV-2 variants, particularly Omicron-BA.5, poorly understood. We investigated the humoral and cellular immune of hospitalized COVID-19 patients during Delta Omicron infection waves. Methods The corresponding variant respective were identified by whole genome sequencing. Humoral analyzed ELISA a cell...
Hematopoietic stem and progenitor cells (HSPCs) can self-renew create committed progenitors, a process supposed to involve asymmetric cell divisions (ACDs). Previously, we had linked the kinetics of CD133 expression with ACDs but failed detect segregation classical epitopes on fixed, mitotic HSPCs. Now, by using novel anti-CD133 antibody (HC7), confirmed occurrence paraformaldehyde-fixed living After showing that HC7 binding does not recognizably affect biological features human HSPCs,...
Abstract In terms of infected human individuals, herpesviruses range among the most successful virus families. Subclinical herpesviral infections in healthy individuals contrast with life-threatening syndromes under immunocompromising and immunoimmature conditions. Based on our finding that cytomegaloviruses interact Cullin Roc ubiquitin ligases (CRLs) context interferon antagonism, we systematically assessed viral dependency CRLs by utilizing drug MLN4924. CRL activity is regulated through...