A5100669996- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Hepatitis Viruses Studies and Epidemiology
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Animal Virus Infections Studies
- Long-Term Effects of COVID-19
- interferon and immune responses
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Bacteriophages and microbial interactions
- Liver Disease and Transplantation
- Viral Infections and Outbreaks Research
- Pediatric Hepatobiliary Diseases and Treatments
- HIV/AIDS drug development and treatment
- Gut microbiota and health
- Viral Infections and Vectors
- Metal and Thin Film Mechanics
- Advanced Surface Polishing Techniques
- Toxin Mechanisms and Immunotoxins
Huazhong University of Science and Technology
2016-2025
Union Hospital
2014-2025
Tsinghua University
2024-2025
Union Hospital
2015-2024
Taiyuan University of Technology
2021-2024
Tongji Hospital
2007-2024
Beijing Tsinghua Chang Gung Hospital
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
First Affiliated Hospital of Dalian Medical University
2023
Dalian Medical University
2023
Background & AimsWe performed a nationwide, retrospective study to determine the incidence and causes of drug-induced liver injury (DILI) in mainland China.MethodsWe collected data on total 25,927 confirmed DILI cases, hospitalized from 2012 through 2014 at 308 medical centers China. We demographic, history, treatment, laboratory, disease severity, mortality all patients. Investigators each site were asked complete causality assessments for case whose diagnosis discharge was (n = 29,478)...
Abstract We have previously shown that Toll-like receptor (TLR)-activated murine nonparenchymal liver cells [(NPC); Kupffer (KC), sinusoidal endothelial (LSEC)] can suppress hepatitis B virus (HBV) replication. Therefore, the aim of this study was to investigate whether HBV has ability counteract TLR-mediated control its Freshly purified hepatocytes and NPCs obtained from C57BL6 mice were stimulated by TLR 1-9 ligands in presence or absence surface antigen (HBsAg), e (HBeAg), virions,...
Hepatitis B virus (HBV) persistence is facilitated by exhaustion of CD8 T cells that express the inhibitory receptor programmed cell death-1 (PD-1). Improvement HBV-specific function has been obtained in vitro inhibiting PD-1/PD-ligand 1 (PD-L1) interaction. In this study, we examined whether vivo blockade PD-1 pathway enhances virus-specific immunity and leads to resolution chronic hepadnaviral infection woodchuck model. The was first cloned, characterized, its expression patterns on from...
Abstract Long-term antibody responses and neutralizing activities in response to SARS-CoV-2 infection are not yet clear. Here we quantify immunoglobulin M (IgM) G (IgG) antibodies recognizing the receptor-binding domain (RBD) of spike (S) or nucleocapsid (N) protein, during a period 6 months from COVID-19 disease onset 349 symptomatic patients who were among first be infected world-wide. The positivity rate magnitude IgM-S IgG-N increase rapidly. High levels IgM-S/N IgG-S/N at 2-3 weeks...
Background: The dynamic changes of lymphocyte subsets and cytokines profiles patients with novel coronavirus disease (COVID-19) their correlation the severity remain unclear. Method: Peripheral blood samples were longitudinally collected from 40 confirmed COVID-19 examined for by flow cytometry cytokine specific immunoassays. Findings: Of enrolled, 13 severe cases showed significant sustained decreases in counts but increases neutrophil than 27 mild cases. Further analysis demonstrated T...
Little is known of how the Toll-like receptor (TLR) system can modulate function non-parenchymal liver cells (NPC) as a major component innate and adaptive immune liver. To investigate diversification TLR signalling pathways in NPC, we isolated Kupffer (KC) sinusoidal endothelial (LSEC) from wild-type C57BL/6 mice examined their responses to TLR1 TLR9 agonists. The data show that KC respond all ligands by producing tumour necrosis factor-alpha (TNF-alpha) or interleukin-6 (IL-6), TLR3 TLR4...
Major advances have been made in understanding the dynamics of humoral immunity briefly after acute coronavirus disease 2019 (COVID-19). However, knowledge concerning long-term kinetics antibody responses convalescent patients is limited. During a one-year period post symptom onset, we longitudinally collected 162 samples from 76 and quantified IgM IgG antibodies recognizing nucleocapsid (N) protein or receptor binding domain (RBD) spike (S). After one year, approximately 90% recovered still...
Abstract Background The dynamic changes of lymphocyte subsets and cytokines profiles patients with novel coronavirus disease (COVID-19) their correlation the severity remain unclear. Methods Peripheral blood samples were longitudinally collected from 40 confirmed COVID-19 examined for by flow cytometry cytokine specific immunoassays. Results Of enrolled, 13 severe cases showed significant sustained decreases in counts but increases neutrophil than 27 mild cases. Further analysis demonstrated...
Tocilizumab has been reported to attenuate the "cytokine storm" in COVID-19 patients. We attempted verify effectiveness and safety of tocilizumab therapy identify patients most likely benefit from this treatment. conducted a randomized, controlled, open-label multicenter trial among The were randomly assigned 1:1 ratio receive either addition standard care or alone. cure rate, changes oxygen saturation interference, inflammation biomarkers observed. Thirty-three randomized group, 32 control...
The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome 2 (SARS-CoV-2) affected over 120 million people and killed 2.7 individuals March 2021. While intermediate interactions between SARS-CoV-2 immune system have been studied extensively, long-term impacts on cellular remain to be analyzed. Here, we comprehensively characterized immunological changes in peripheral blood mononuclear cells 49 COVID-19-convalescent (CI) comparison 27 matched...
We have previously shown that poly(I:C) activates murine hepatic cells to produce interferon (IFN) and suppresses hepatitis B virus (HBV) replication in vitro. Therefore, we addressed whether is able induce the clearance of HBV vivo. The chronic mouse model was established by hydrodynamic injection (HI) pAAV-HBV1.2 into tail veins wild-type IFN-α/βR-, IFN-γ-, CXCR3-deficient C57BL/6 mice. Fourteen days post-HI pAAV-HBV1.2, mice were administered intraperitoneal injection, intramuscular or...
A variety of amino acid substitutions, such as K122I and G145R, have been identified around or within the a determinant hepatitis B surface antigen (HBsAg), impair HBsAg secretion antibody binding, may be responsible for immune escape in patients. In this study, we examined how different substitutions at positions 122 145 influence expression, secretion, recognition by anti-HBs antibodies. The results showed that hydrophobicity, presence phenyl group, charges side chain residues position...
Abstract Background Patients with severe Coronavirus Disease 2019 (COVID-19) will progress rapidly to acute respiratory failure or death. We aimed develop a quantitative tool for early predicting mortality risk of patients COVID-19. Methods 301 confirmed COVID-19 admitted Main District and Tumor Center the Union Hospital Huazhong University Science Technology (Wuhan, China) between January 1, 2020 February 15, were enrolled in this retrospective two-centers study. Data on patient demographic...
Abstract Long-term antibody responses and neutralizing activities following SARS-CoV-2 infections have not yet been elucidated. We quantified immunoglobulin M (IgM) G (IgG) antibodies recognizing the receptor-binding domain (RBD) of spike (S) or nucleocapsid (N) protein, during a period six months COVID-19 disease onset in 349 symptomatic patients, which were among first world-wide being infected. The positivity rate magnitude IgM-S IgG-N increased rapidly. High levels IgM-S/N IgG-S/N at 2-3...
While the immunogenicity of inactivated vaccines against coronavirus disease 2019 (COVID‐19) has been characterized in several well-conducted clinical trials, real-world evidence concerning immune responses severe acute respiratory syndrome 2 (SARS‐CoV‐2) raised by such is currently missing. Here, we comprehensively various parameters SARS-CoV-2-specific cellular and humoral induced COVID-19 126 individuals under conditions. After two doses vaccination, S-receptor binding domain IgG (S-RBD...
Commensal gut microbiota protects the immune defense of extra-intestinal organs. Gut depletion by antibiotics can impair host antiviral responses and alter hepatitis B virus (HBV) infection outcomes. However, how modulates response in liver remains unclear. Here, mice were treated with broad-spectrum to deplete microbiota. integrity was evaluated, translocation live commensal bacteria their components into investigated. An HBV model established evaluate impairment after depletion. We found...
Electrolytic plasma polishing (EPPo) is an advanced metal surface finishing technology with high quality and environmental protection that has broad application prospects in the biomedical field. However, effect of EPPo on properties such as corrosion resistance wettability titanium alloys remains to be investigated. This paper investigated changes roughness, morphology, microstructure, chemical composition Ti6Al4V alloy by their effects resistance, wettability, residual stress. The results...