A5027480329- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Hematopoietic Stem Cell Transplantation
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Renal Transplantation Outcomes and Treatments
- Virus-based gene therapy research
- Animal Nutrition and Physiology
- Mycotoxins in Agriculture and Food
- CAR-T cell therapy research
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Pluripotent Stem Cells Research
- Cancer Immunotherapy and Biomarkers
- Long-Term Effects of COVID-19
- Mesenchymal stem cell research
- Herpesvirus Infections and Treatments
- Hepatitis B Virus Studies
- Rabbits: Nutrition, Reproduction, Health
- MicroRNA in disease regulation
University of Duisburg-Essen
2016-2025
Essen University Hospital
2016-2025
Hamburgische Schiffbau-Versuchsanstalt (Germany)
2022-2024
Heinrich Heine University Düsseldorf
2003-2024
Düsseldorf University Hospital
2024
Magyar Agrár- és Élettudományi Egyetem
2021-2023
University of Kapsovar
2011-2022
Deutschen Konsortium für Translationale Krebsforschung
2014-2021
German Cancer Research Center
2015-2021
Institute for Transfusion Medicine
2009-2017
Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c)...
Although the initial concepts of stem cell therapy aimed at replacing lost tissue, more recent evidence has suggested that and progenitor cells alike promote postischemic neurological recovery by secreted factors restore injured brain's capacity to reshape. Specifically, extracellular vesicles (EVs) derived from such as exosomes have recently been mediate restorative effects. In order define whether EVs indeed improve impairment brain remodeling, we systematically compared effects...
Abstract There is a constant need to increase productivity in aquaculture, particularly improve growth rate, feed utilization as well stress resistance of fish. Because consumer concerns and strict regulations many countries, the use synthetic chemicals, hormones antibiotics becoming unviable natural compounds are more acceptable public. A wide variety chemical found plants, them have been shown beneficial effects on appetite, immune status fish acting through different mechanisms....
Extracellular vesicles (EVs) mediate targeted cellular interactions in normal and pathophysiological conditions are increasingly recognised as potential biomarkers, therapeutic agents drug delivery vehicles. Based on their size biogenesis, EVs classified exosomes, microvesicles apoptotic bodies. Due to overlapping ranges the lack of specific markers, these classes cannot yet be distinguished experimentally. Currently, it is a major challenge field define robust sensitive technological...
Preterm brain injury is a major cause of disability in later life, and may result motor, cognitive behavioural impairment for which no treatment currently available. The aetiology considered as multifactorial, one underlying key player inflammation leading to white grey matter injury. Extracellular vesicles secreted by mesenchymal stem/stromal cells (MSC-EVs) have shown therapeutic potential regenerative medicine. Here, we investigated the effects MSC-EV on microstructure maturation,...
Extracellular vesicles (EVs) provide a complex means of intercellular signalling between cells at local and distant sites, both within different organs. According to their cell-type specific signatures, EVs can function as novel class biomarkers for variety diseases, be used drug-delivery vehicles. Furthermore, from certain cell types exert beneficial effects in regenerative medicine immune modulation. Several techniques are available harvest various body fluids or culture supernatants....
Abstract We investigated immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among a group of convalescent, potential blood donors in Germany who had PCR-confirmed SARS-CoV-2 infection. Sixty days after onset symptoms, 13/78 (17%) study participants borderline or negative results to an ELISA detecting IgG the S1 protein SARS-CoV-2. analyzed with infection strong antibody (ratio >3) as positive controls and without symptoms household contact infected patients...
CD8(+) T lymphocytes can kill autologous melanoma cells, but their activity is impaired when poorly immunogenic tumor phenotypes evolve in the course of disease progression. Here, we analyzed three consecutive lesions obtained within one year developing stage IV for recognition by cells.One skin (Ma-Mel-48a) and two lymph node (Ma-Mel-48b, Ma-Mel-48c) metastases were T-cell infiltration. Melanoma cell lines established from respective characterized, determining T-cell-stimulatory capacity,...
The classical model of hematopoiesis predicts a dichotomous lineage restriction multipotent hematopoietic progenitors (MPPs) into common lymphoid (CLPs) and myeloid (CMPs). However, this idea has been challenged by the identification retaining partial potential (e.g., LMPPs), implying that granulocytes can arise within both branches. Here, we resolve issue using cell-surface CD133 expression to discriminate functional progenitor populations. We show eosinophilic basophilic as well...
Abstract We investigated immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among a group of convalescent, potential blood donors in Germany who had PCR-confirmed SARS-CoV-2 infection. Sixty days after onset symptoms, 13/78 (17%) study participants borderline or negative results to an ELISA detecting IgG the S1 protein SARS-CoV-2. analyzed with infection strong antibody (ratio >3) as positive controls and without symptoms household contact infected patients...
Extracellular vesicles (EVs) have been discussed as a diagnostic tool for minimal residual disease (MRD) evaluation in breast cancer (BC) addition to the analysis of circulating tumor cells (CTCs). Therefore, we investigated EV levels surrogate markers monitoring and prediction prognosis primary, non-metastatic, locally advanced BC patients. EVs were enriched from blood samples patients before after neoadjuvant chemotherapy (NACT) healthy females. marker expression was performed sizes...
Abstract Expression of the non-classical human leukocyte antigen-G (HLA-G) promotes cancer progression in various malignancies including epithelial ovarian (EOC). As single nucleotide polymorphisms (SNPs) HLA-G 3′ untranslated region (UTR) regulate expression, we investigated 3′UTR haplotypes arranged by SNPs healthy controls (n = 75) and primary EOC patients 79) determined soluble (sHLA-G) levels. Results were related to clinical status outcome. Although haplotype frequencies similar...
The cellular response to SARS-CoV-2 vaccination and infection in allogeneic hematopoietic stem cell transplant (HSCT) recipients is not yet clear. In the current study, HSCT prior post were tested for SARS-CoV-2-specific humoral immunity. Antibodies against spike (S) 1 assessed by Anti-SARS-CoV-2 IgG ELISA (Euroimmun). Cellular immunity was analyzed an house interferon-gamma ELISpot T-SPOT.COVID (Oxford Immunotec), using altogether seven antigens. 117 patients vaccinated twice, antibodies...
Extracellular vesicles (EVs) harvested from conditioned media of human mesenchymal stromal cells (MSCs) suppress acute inflammation in various disease models and promote regeneration damaged tissues. After successful treatment a patient with steroid-refractory graft-versus-host (GVHD) using EVs prepared bone marrow-derived MSCs, this study focused on improving the MSC-EV production for clinical application.
Human mesenchymal stromal cell (MSC)-derived extracellular vesicles (EV) revealed neuroprotective potentials in various brain injury models, including neonatal encephalopathy caused by hypoxia-ischemia (HI). However, for clinical translation of an MSC-EV therapy, scaled manufacturing strategies are required, which is challenging with primary MSCs due to inter- and intra-donor heterogeneities. Therefore, we established a clonally expanded immortalized human MSC line (ciMSC) compared the...
The most potent vasoconstrictor known, endothelin-1, is currently considered to mediate cerebral vasospasm in subarachnoid hemorrhage (SAH), which can cause delayed ischemia. In our study, we performed clinical and vitro experiments investigate the origin mechanisms of secretion endothelin-1 SAH.Endothelin-1 markers inflammatory host response (interleukin [IL]-1ss, IL-6, tumor necrosis factor-alpha) were comparatively quantified cerebrospinal fluid (CSF) SAH patients control subjects,...