A5090119518- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Mesenchymal stem cell research
- Acute Lymphoblastic Leukemia research
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Pluripotent Stem Cells Research
- Bacterial Genetics and Biotechnology
- Histone Deacetylase Inhibitors Research
- Hemoglobinopathies and Related Disorders
- CAR-T cell therapy research
- Multiple Myeloma Research and Treatments
- T-cell and B-cell Immunology
- Metabolomics and Mass Spectrometry Studies
- Lymphoma Diagnosis and Treatment
- Virus-based gene therapy research
- Immune cells in cancer
- Protein Degradation and Inhibitors
- Erythrocyte Function and Pathophysiology
- Renal and related cancers
- RNA Interference and Gene Delivery
University Hospital Leipzig
2015-2024
Leipzig University
2013-2023
University of Washington
2021
Statistical Service
2019
Leeds Teaching Hospitals NHS Trust
2013
Bielefeld University
2010
Cancer Research UK Manchester Institute
1991-2007
The Christie NHS Foundation Trust
1994-2007
Max Planck Institute for Medical Research
2006
Johannes Gutenberg University Mainz
2006
We have isolated and characterized both cDNA genomic DNA of the mouse lysozyme M gene. Derivation amino acid sequence from nucleotide sequences revealed six positions in carboxyl terminus that differ partial previously published. The differential detection specific mRNAs closely related P genes has different but overlapping tissue specificities expression. gene is expressed weakly myeloblasts, moderately immature macrophages, strongly mature macrophages macrophage-rich tissues, while high...
Secreted hemolysins were extremely common among clinical isolates of Proteus mirabilis, vulgaris, and Morganella morganii, hemolytic activity was either cell associated or free. Southern hybridization total DNA from to cloned regions the Escherichia coli alpha-hemolysin (hly) determinant showed clear but incomplete homology between genes encoding production in four species. One two E. secretion genes, hlyD, hybridized only with P. vulgaris M. which produced cell-free hemolysis, not that...
Background. To evaluate the pathologic characteristics of primary tumor relative to local control, survival, and freedom from distant metastases, an analysis was performed 172 patients with ductal carcinoma in situ (intraductal carcinoma) breast treated breast-conserving surgery definitive irradiation. Methods. The clinical records pathology slides were reviewed women irradiation at multiple institutions Europe United States. Central review by one pathologist without knowledge outcome....
The classical model of hematopoiesis predicts a dichotomous lineage restriction multipotent hematopoietic progenitors (MPPs) into common lymphoid (CLPs) and myeloid (CMPs). However, this idea has been challenged by the identification retaining partial potential (e.g., LMPPs), implying that granulocytes can arise within both branches. Here, we resolve issue using cell-surface CD133 expression to discriminate functional progenitor populations. We show eosinophilic basophilic as well...
An analysis of 259 women with 261 treated breasts from nine institutions in Europe and the United States was performed to determine 10-year results treatment intraductal carcinoma breast definitive irradiation. All patients had undergone complete gross excision primary carcinoma, irradiation delivered all cases. The median follow-up time 78 months (range, 11 197 months). actuarial overall survival rate 94%, cause-specific (including deaths only breast) 97%. freedom distant metastases 96%....
The safety and efficacy of azacitidine (5-day schedule) were assessed in a multicenter study 40 patients (median age 72 years) with acute myeloid leukemia (AML) medically unfit for (n = 20) or resistant to chemotherapy from April October 2008. Median marrow blasts 42%. After median follow-up 13 months, response (complete remission [CR]/partial [PR]/hematologic improvement [HI]) was 50% 10% newly diagnosed relapsed/refractory patients, respectively (p 0.008). time-to-response 2.5 months...
The risk profile and prognosis of patients with myelofibrosis is well described by the Dynamic International Prognostic Scoring System categorization. Allogeneic stem cell transplantation considered for intermediate-2/high disease. However, indicators after are still lacking. Seventy simultaneously collected pairs trephine blood samples were quantified JAK2 p.V617F allele burden to compare test sensitivity. course 30 p.V617F-positive myeloproliferative neoplasia was correlated...
Patients with myelodysplastic syndrome and ring sideroblasts (MDS-RS) present symptomatic anemia due to ineffective erythropoiesis that impedes their quality of life increases morbidity. More than 80% patients MDS-RS harbor splicing factor 3B subunit 1 (SF3B1) mutations, the founder aberration driving disease. Here, we report how mis-splicing coenzyme A synthase ( COASY ), induced by mutations in SF3B1 , affects heme biosynthesis erythropoiesis. Our data revealed was up-regulated during...
Lineage specification of hematopoietic stem cells is considered a progressive restriction in lineage potential. This view consistent with observations that differentiation and preceded by low-level coexpression specific, potentially antagonistic genes early progenitor cells. coexistence, commonly referred to as priming, disappears the course when certain lineage-restricted are upregulated while others downregulated. Based on this phenomenological description, we propose quantitative model...
Very small embryonic-like (VSEL) cells have been described as putatively pluripotent stem present in murine bone marrow and human umbilical cord blood (hUCB) such are of high potential interest for regenerative medicine. However, there remain some questions concerning the precise identity properties VSEL cells, particularly those derived from hUCB. For this reason, we carried out an extensive characterisation purified populations a large number UCB samples. Consistent with previous report,...
Advancements in comprehending myelodysplastic neoplasms (MDS) have unfolded significantly recent years, elucidating a myriad of cellular and molecular underpinnings integral to disease progression. While inclusions into prognostic models substantively advanced risk stratification, revelations emphasized the pivotal role immune dysregulation within bone marrow milieu during MDS evolution. Nonetheless, immunotherapy for has not experienced breakthroughs seen other malignancies, partly...
Summary The gene encoding the c. 105 kD secreted haemolysin protein of porcine pathogen Actinobacillus pleuropneumoniae serotype 1 has been isolated by screening a lambda gt11 expression library in Escherichia coli with antiserum raised against wild‐type protein. A derivative recombinant DNA pJFF702 expressed hlylA from pUC19 lac promoter but resulting I remained within E. cell and was haemolytically inactive. Export intracellular A. prohaemolysin out into medium achieved presence trans...
Using a plasmid containing transcriptional fusion in which the E.coli galk gene is expressed from trfB promoter of broad host range RK2 we show that transcription repressed by products both and korB genes as have previously predicted sequence homology trfA promoters fact regulated . These loci, are normally transcribed promoter. Thus trfBincCkorB operon doubly autogeneously regulated. In addition, describe isolation characterization mutant has become insensitive to repression result point...
Background. Human bone marrow contains hematopoietic stem cells and stroma known as mesenchymal (MSC). MSC are with the morphological features of fibroblasts, which, in addition to their nursing function for cells, retain ability differentiate into cartilage, bone, fat, muscle, tendon have an important immunmodulatory function. To understand more detail engraftment immune modulation after cell transplantation, we investigated donor engraft transplantation dependency conditioning regimen...