A5009769102- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Acute Myeloid Leukemia Research
- Cancer Genomics and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Lymphoma Diagnosis and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Eosinophilic Disorders and Syndromes
- Hematopoietic Stem Cell Transplantation
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Viral-associated cancers and disorders
- Circular RNAs in diseases
- Biomarkers in Disease Mechanisms
- Immune cells in cancer
- Sarcoma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Global Cancer Incidence and Screening
University of Cologne
2003-2024
Martin Luther University Halle-Wittenberg
2015-2024
University Hospital in Halle
2015-2023
RWTH Aachen University
2022
University of Gezira
2017
Weill Cornell Medical College in Qatar
2017
Martha-Maria Hospital
2016
University Hospital Leipzig
2008-2015
Data Harbor (United States)
2015
Leipzig University
2008-2014
Inter-patient variability and the similarity of healthy leukemic stem cells (LSCs) have impeded characterization LSCs in acute myeloid leukemia (AML) their differentiation landscape. Here, we introduce CloneTracer, a novel method that adds clonal resolution to single-cell RNA-seq datasets. Applied samples from 19 AML patients, CloneTracer revealed routes differentiation. Although residual preleukemic dominated dormant cell compartment, active resembled counterpart retained erythroid...
Evaluation of T lymphocyte frequency provides prognostic information for patients with oral squamous cell cancer (OSCC). However, the effect simultaneously evaluating and assessing suppressive elements defects in antigen-processing machinery (APM) has not been clarified. Simultaneous characterization CD3+, CD8+, FoxP3+, CD163+, PD-L1+ cells using multispectral imaging was performed on sections from 119 HPV- OSCC. Expression β2-microglobulin, MHC class I heavy chain, large multifunctional...
Autopsies are an important tool in medicine, dissecting disease pathophysiology and causes of death. In COVID-19, autopsies revealed e.g., the effects on pulmonary (micro)vasculature or nervous system, systemic viral spread, interplay with immune system. To facilitate multicentre autopsy-based studies provide a central hub supporting autopsy centres, researchers, data analyses reporting, April 2020 German COVID-19 Autopsy Registry (DeRegCOVID) was launched. The electronic registry uses...
<h3>Importance</h3> In metastatic esophagogastric adenocarcinoma (EGA), the addition of programmed cell death 1 (PD-1) inhibitors to chemotherapy has improved outcomes in selected patient populations. <h3>Objective</h3> To investigate efficacy trastuzumab and PD-1 with cytotoxic T-lymphocyte–associated antigen 4 (CTLA-4) or FOLFOX first-line treatment advanced<i>ERBB2</i>-positive EGA. <h3>Design, Setting, Participants</h3> This phase 2 multicenter, outpatient, randomized clinical trial...
Control of pathogens by formation abscesses and granulomas is a major strategy the innate immune system, especially when effector mechanisms adaptive immunity are insufficient. We show in human listeriosis that DCs expressing indoleamine 2,3-dioxygenase (IDO), together with macrophages, cellular components suppurative vivo. Induction IDO cell-autonomous response to Listeria monocytogenes infection was also observed other granulomatous infections intracellular bacteria, such as Bartonella...
Abstract: Megakaryocytes are difficult to isolate because of their fragility, tendency aggregate, and varying sizes. For purification cells at different stages maturation sizes (ploidy classes) we developed an immunomagnetic cell sorting method (MACS) enrich the whole spectrum megakaryocytic lineage. The use small magnetic beads coupled various antibodies labelling with fluorescent allowed direct analysis enrichment evaluation isolated fraction without further staining or detachment...
Interleukin-10 (IL-10) is an important regulator of immune responses secreted by different cell types. We have previously shown that mice with selective inactivation the IL-10 gene in T cells suffer from deregulated similar to those observed IL-10(-/-) animals. Unlike mice, however, cell-specific mutants do not mount enhanced innate response LPS, which must, therefore, be subject control non-T cells. Herein we show subcutaneous injection causes moderate local inflammation WT and mutant...
MicroRNAs have recently taken centre stage as short non-coding RNAs that regulate mRNA expression.To assess the feasibility of using microRNA techniques on routinely processed tissues, accessibility two representative microRNAs was examined by real-time quantitative PCR in 86 human formalin-fixed paraffin-embedded (FFPE) samples from liver, breast, bone marrow, lymphatic tissues and colon. Murine liver used to analyse influence fixation time different fixatives.High-quality successfully...
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare haematopoietic malignancy characterized by dismal prognosis and overall poor therapeutic response. Since the biology of BPDCN barely understood, our study aims to shed light on genetic make-up these highly malignant tumors. Using targeted high-coverage massive parallel sequencing, we investigated 50 common cancer genes in 33 samples. We detected point mutations NRAS (27.3% cases), ATM (21.2%), MET, KRAS, IDH2, KIT (9.1% each),...
Abstract Purpose: In gastrointestinal stromal tumor (GIST), there is no biomarker available that indicates success or failure of therapy. We hypothesized tumor-specific v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (CKIT)- platelet-derived growth factor receptor-α (PDGFRA)–mutant DNA fragments can be detected and quantified in plasma samples patients with GIST. Experimental Design: prospectively collected 291 from 38 subjects GIST harboring activating mutations CKIT PDGFRA...
The risk profile and prognosis of patients with myelofibrosis is well described by the Dynamic International Prognostic Scoring System categorization. Allogeneic stem cell transplantation considered for intermediate-2/high disease. However, indicators after are still lacking. Seventy simultaneously collected pairs trephine blood samples were quantified JAK2 p.V617F allele burden to compare test sensitivity. course 30 p.V617F-positive myeloproliferative neoplasia was correlated...
Background In patients with microsatellite stable (MSS) metastatic colorectal cancer (mCRC), immune checkpoint blockade is ineffective, and combinatorial approaches enhancing immunogenicity need exploration. Methods We treated 43 predominantly RAS/BRAF wild-type mCRC on a phase II trial combining chemotherapy the epidermal growth factor receptor antibody cetuximab programmed cell death ligand 1 (PD-L1) avelumab. performed next-generation gene panel sequencing for mutational typing of tumors...