A5014563036- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Infant Nutrition and Health
- Proteoglycans and glycosaminoglycans research
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Advanced Proteomics Techniques and Applications
- Muscle Physiology and Disorders
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Helicobacter pylori-related gastroenterology studies
- Cardiomyopathy and Myosin Studies
- Inflammatory Myopathies and Dermatomyositis
- Metabolism and Genetic Disorders
- Erythrocyte Function and Pathophysiology
- Genetic factors in colorectal cancer
- Mass Spectrometry Techniques and Applications
- Escherichia coli research studies
- Genetic Neurodegenerative Diseases
- Breastfeeding Practices and Influences
- Caveolin-1 and cellular processes
- Invertebrate Immune Response Mechanisms
- Bacterial Infections and Vaccines
University of Cologne
2015-2024
University Hospital Cologne
1985-2022
Martin Luther University Halle-Wittenberg
2012
Klinik und Poliklinik für Neurologie
2012
Otto-von-Guericke University Magdeburg
2010
Institute of Molecular Biology
2010
University Hospital in Halle
2010
Science Applications International Corporation (United States)
2005
Frederick National Laboratory for Cancer Research
2005
National Cancer Institute
2005
Comprehensive proteomic profiling of the human adipocyte secretome identified dipeptidyl peptidase 4 (DPP4) as a novel adipokine. This study assessed functional implications adipokine DPP4 and its association to metabolic syndrome.Human adipocytes skeletal smooth muscle cells were used monitor release assess effects soluble on insulin signaling. In lean obese subjects, depot-specific expression from adipose tissue explants determined correlated parameters syndrome.Fully differentiated...
Fibulins are a family of five extracellular matrix proteins characterized by tandem arrays epidermal growth factor-like domains and C-terminal fibulin-type module. They widely distributed often associated with vasculature elastic tissues. In this study, we expressed the three more recently identified members, fibulin-3, fibulin-4, fibulin-5, as recombinant in mammalian cells. The purified showed short rod structures ∼20 nm globule at one end, after rotary shadowing electron microscopy. Two...
Recently, an alternative route to the proteasomal protein-degradation pathway was discovered that specifically targets transmembrane proteins marked with a single ubiquitin endosomal multivesicular body (MVB) and,subsequently, vacuole (yeast) or lysosome (animals), where they are degraded by proteases. Vps23p/TSG101 is key component of ESCRT I-III machinery in yeast and animals recognizes mono-ubiquitylated sorts them into MVB. Here, we report Arabidopsis ELCH(ELC) gene encodes homolog,...
Apically expressed human MUC1 is known to become endocytosed and either re-enter the secretory pathway for recycling plasma membrane or be exported by cells via formation of multi-vesicular bodies release exosomes. By using recombinant fusion-tagged as a bait protein we followed an anti-myc affinity-based approach isolating subpopulations lipid rafts from membranes exosomes MCF-7 breast cancer cells. MUC1(+) were not only found contain genuine raft proteins (flotillin-1, prohibitin, G...
Abstract Purpose: Overexpression of MUC1 and cytosolic interaction the mucin with β-catenin are claimed to be involved in colorectal carcinogenesis. In vitro data published recently suggest that overexpression results an increase steady state levels nuclear β-catenin. We tried elucidate coexpression both molecules cancer demonstrate possible correlations clinical, pathological, prognostic data. Experimental Design: An immunohistochemical double staining study was performed characterize...
In contrast to protein antigens, processing of glycoproteins by dendritic cells (DCs) for presentation T has not been well studied. We developed mouse cell hybridomas study and the tumor antigen MUC1 as a model glycoprotein. is expressed on surface secreted human adenocarcinomas. Circulating soluble available uptake, processing, DCs in vivo better understanding how that process functions case glycosylated antigens may shed light antitumor immune responses could be initiated against this show...
Control of pathogens by formation abscesses and granulomas is a major strategy the innate immune system, especially when effector mechanisms adaptive immunity are insufficient. We show in human listeriosis that DCs expressing indoleamine 2,3-dioxygenase (IDO), together with macrophages, cellular components suppurative vivo. Induction IDO cell-autonomous response to Listeria monocytogenes infection was also observed other granulomatous infections intracellular bacteria, such as Bartonella...
Knowledge about the O-linked glycan chains of tumor-associated MUC1 is primarily based on enzymatic and immunochemical evidence. To obtain structural information to overcome limitations by scarcity endogenous mucin, we expressed a recombinant glycosylation probe corresponding six tandem repeats in four breast cancer cell lines. Comparative analyses O-glycan profiles were performed after hydrazinolysis normal phase chromatography 2-aminobenzamide-labeled glycans. Except for general reduction...
TFF3 is a secretory peptide belonging to the trefoil factor family with predicted size of 59 amino acid residues containing seven cysteine residues. It predominantly expressed in intestinal goblet cells where it plays key role mucosal regeneration and repair processes. In course these studies, human colonic was shown exist mainly as high molecular weight heteromer. Purification this heteromer characterization by LC-ESI-MS/MS analysis identified IgG Fc binding protein (FCGBP) disulfide-linked...
Abstract The C-terminal pro-fibrillin-1 propeptide asprosin is described as white adipose tissue derived hormone that stimulates rapid hepatic glucose release and activates hunger-promoting hypothalamic neurons. Numerous studies proposed correlations of levels with clinical parameters. However, the enormous variability reported serum plasma illustrates need for sensitive reliable detection methods in samples. Here we report on newly developed biochemical concentration several body fluids...
A highly immunogenic peptide motif within the tandem repeat domain of MUC1 mucin is assumed to be exposed during development breast cancer due altered O‐glycosylation. To elucidate structural aspects these changes, we have isolated and analysed integrated or secretory glycoforms from carcinoma cell lines solid tumors human milk. The buoyant densities measured in CsCl gradients for cells revealed heterogeneity physicochemical species a significant reduction their carbohydrate contents...
The expression of MUC1 and MUC2 mucin peptide core antigens in gastric carcinomas was studied by immunohistochemistry to determine correlations with TNM stage histo-pathological classifications as well a possible prognostic impact. Paraffin-embedded specimens from 128 minimal follow-up 5 years were immunostained. In addition polyclonal antiserum generated against polymorphic epithelial (MUC1) human milk, 2 monoclonal antibodies (MAbs), HMFG2 (anti-MUC1) 4FI (anti-MUC2), applied. Reactivity...
The immunostimulatory outcome of the interactions many pathogens with dendritic cells (DCs) has been well characterized. There are fewer examples similar between DCs and self-molecules, especially abnormal self-proteins such as tumor Ags, their effects on DC function immune response. We show that human epithelial cell Ag MUC1 mucin is recognized in its aberrantly glycosylated form by immature myeloid both a chemoattractant (through polypeptide core) maturation activation signal carbohydrate...
The therapeutic efficacy of recombinant antibodies such as single-chain Fv fragments and small bispecific or bifunctional molecules is often limited by rapid elimination from the circulation because their size. Here, we have investigated effects N-glycosylation on activity pharmacokinetics a diabody (scDb CEACD3) developed for retargeting cytotoxic T cells to CEA-expressing tumor cells. We could show that introduction sequons into flanking linker C-terminal extension results in production...
Mutations of the human valosin-containing protein gene cause autosomal-dominant inclusion body myopathy associated with Paget disease bone and frontotemporal dementia. We identified strumpellin as a novel binding partner. Strumpellin mutations have been shown to hereditary spastic paraplegia. demonstrate that is ubiquitously expressed present in cytosolic endoplasmic reticulum cell fractions. Overexpression or ablation wild-type caused significantly reduced wound closure velocities healing...
A variety of genetic variations in the galactose-1-phosphate uridyltransferase (GALT) gene cause profound activity loss enzyme and acute toxic effects mediated by accumulating metabolic intermediates galactose newborns induced dietary galactose. However, even on a severely galactose-restricted diet, patients develop serious long-term complications CNS ovaries, which may result from damaging perturbations cell biology caused endogenously synthezised Under stress, cosubstrate GALT,...
Human bone sialoprotein (BSP) comprises 15% of the total noncollagenous proteins in and is thought to be involved mineralization remodeling. Recent data suggest a role for BSP breast cancer development metastases. We have produced full-length recombinant human cell line purified protein from retaining native structure with proper folding post-translational modifications. Mass spectrometry bone-derived revealed an average mass 49 kDa 57 kDa. The modifications contribute 30–40%. Carbohydrate...