A5064247559- Bacterial Infections and Vaccines
- Infant Nutrition and Health
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Barrier Structure and Function Studies
- Immune Response and Inflammation
- Escherichia coli research studies
- Streptococcal Infections and Treatments
- Cerebrospinal fluid and hydrocephalus
- Neonatal and Maternal Infections
- Glycosylation and Glycoproteins Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytomegalovirus and herpesvirus research
- Infectious Encephalopathies and Encephalitis
- Connexins and lens biology
- Neonatal Respiratory Health Research
- Gut microbiota and health
- Infectious Diseases and Tuberculosis
- Immunodeficiency and Autoimmune Disorders
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience of respiration and sleep
- Mitochondrial Function and Pathology
- Breastfeeding Practices and Influences
- Clinical Nutrition and Gastroenterology
Heidelberg University
2016-2025
University Hospital Heidelberg
2016-2025
University Medical Centre Mannheim
2015-2024
Heidelberg University
2016-2022
Klinik und Poliklinik für Kinder- und Jugendmedizin
2010-2019
Uniwersytecki Szpital Dziecięcy
2015-2018
Charité - Universitätsmedizin Berlin
2006-2016
Paul Ehrlich Institut
2016
Robert Koch Institute
2016
Engineering Associates (United States)
2016
X-linked severe combined immunodeficiency is a recessive hereditary disease characterized by and persistent infections starting in the first months of life associated with diarrhea failure to thrive.1 Affected infants almost invariably present an absence T cells natural killer cells, normal or elevated B-cell counts, hypogammaglobulinemia. This rapidly fatal without bone marrow transplantation.2 The locus has been mapped Xq12–13,3 genetic defect identified as mutation γ chain interleukin-2...
We investigated the presence of factors in human milk that inhibit invasion pathogenic bacteria. The effect fat globule membrane (HMFGM) components on adhesion cloned S-fimbriated Escherichia coli to buccal epithelial cells was analyzed. S fimbriae are a common feature E. strains causing sepsis and meningitis newborns bound epithelia via sialyl-(alpha-2-3)galactoside structures. Human globules (HMFG) could be agglutinated by above-mentioned Agglutination inhibited fetuin, glycophorin, alpha...
Respiratory viruses are the main cause of acute respiratory tract infection (ARI) in children. Real-time polymerase chain reaction (PCR) technology is highly practicable for rapid detection viral pathogens. The simultaneous a broad spectrum enables diagnosis and evaluation coinfection ARI.A 1-step real-time PCR was developed 12 (10 RNA 2 DNA viruses) clinical samples. Clinical samples from 254 children admitted to Departments Pediatrics with ARI during 10-month period were tested.Respiratory...
Histo-blood group antigens (HBGAs) are important binding factors for norovirus infections. We show that two human milk oligosaccharides, 2'-fucosyllactose (2'FL) and 3-fucosyllactose (3FL), could block from to surrogate HBGA samples. found 2'FL 3FL bound at the equivalent pockets on capsid using X-ray crystallography. Our data revealed structurally mimic HBGAs. These results suggest might act as naturally occurring decoys in humans.
Abstract Background The brain barriers establish compartments in the central nervous system (CNS) that significantly differ their communication with peripheral immune system. In this function they strictly control T-cell entry into CNS. T cells can reach CNS by either crossing endothelial blood–brain barrier (BBB) or epithelial blood-cerebrospinal fluid (BCSFB) of choroid plexus (ChP). Objective Analysis cellular and molecular mechanisms involved migration different human CD4 + subsets...
Abstract The choroid plexus (ChP) has been suggested as an alternative central nervous system (CNS) entry site for CCR6 + Th17 cells during the initiation of experimental autoimmune encephalomyelitis (EAE), animal model multiple sclerosis (MS). To advance our understanding importance ChP in orchestrating CNS immune cell neuroinflammation, we here directly compared accumulation CD45 subsets ChP, brain and spinal cord at different stages EAE by flow cytometry. We found that harbors high...
ABCA3 mutations are known to cause fatal surfactant deficiency.We studied protein expression in full-term newborns with unexplained respiratory distress syndrome (URDS) as well the relevance of for homeostasis.Lung tissue infants URDS was analyzed type II pneumocytes. Coding exons gene were sequenced. Surfactant by immunohistochemistry, immunoelectron microscopy, and Western blotting.ABCA3 found be greatly reduced or absent 10 14 URDS. Direct sequencing revealed distinct clustering within...
Abstract Although chronic courses of norovirus infection have been described in immunocompromised patients, little is known about noroviral shedding and correlation with clinical symptoms these patients. In this report, the quantitative excretion nine pediatric patients hematologic oncologic disorders prolonged gastroenteritis were investigated. a retrospective study multiple fecal samples from cancer examined by one‐step real‐time PCR. Clinical data reviewed virological correlated symptoms....
Acute bacterial meningitis is a life-threatening disease in humans. Discussed as entry sites for pathogens into the brain are blood-brain and blood-cerebrospinal fluid barrier (BCSFB). Although human microvascular endothelial cells (HBMEC) constitute well established vitro model barrier, until now no reliable system presenting BCSFB has been developed. Here, we describe first time functional based on choroid plexus papilloma (HIBCPP), which display typical hallmarks of expression junctional...
A critical point during the course of central nervous system infection is influx leukocytes from blood into brain across blood-brain barrier (BBB) and blood-cerebrospinal fluid (BCSFB). However, experimental in vitro models to investigate leukocyte transmigration cultured choroid plexus epithelial cells have been lacking so far.We developed a porcine human "inverted" culture insert that enables specifically physiologically relevant basolateral side. The use primary (PCPEC) papilloma...
Streptococcus suis is an important meningitis-causing pathogen in pigs and humans. Neutrophil extracellular traps (NETs) have been identified as host defense mechanism against different pathogens. Here, NETs were detected the cerebrospinal fluid (CSF) of S. suis-infected piglets despite presence active nucleases. To study NET-formation NET-degradation after transmigration neutrophils through choroid plexus epithelial cell barrier, a previously described model human blood-CSF barrier was...
Zika virus (ZIKV) can infect and cause microcephaly Zika-associated neurological complications in the developing fetal adult brains. In terms of pathogenesis, a critical question is how ZIKV overcomes barriers separating brain from circulation gains access to central nervous system (CNS). Despite importance route utilizes cross CNS remains unclear. Here we show that mouse models, ZIKV-infected cells initially appeared periventricular regions brain, including choroid plexus meninges, prior...
Abstract The choroid plexus (CP) is a key regulator of the central nervous system (CNS) homeostasis through its secretory, immunological and barrier properties. Accumulating evidence suggests that CP plays pivotal role in pathogenesis multiple sclerosis (MS), but underlying mechanisms remain largely elusive. To get comprehensive view on MS, we studied transcriptomic alterations human progressive MS non-neurological disease controls using RNA sequencing. We identified 17 genes with...