A5039701528- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- Cell Image Analysis Techniques
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Calibration and Measurement Techniques
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- Cancer Genomics and Diagnostics
- Advanced Fluorescence Microscopy Techniques
- Optical Imaging and Spectroscopy Techniques
- AI in cancer detection
- Radiomics and Machine Learning in Medical Imaging
- Immune cells in cancer
- Atmospheric Ozone and Climate
- Genetics, Bioinformatics, and Biomedical Research
- Geophysics and Gravity Measurements
- Gene expression and cancer classification
- Spacecraft and Cryogenic Technologies
- Optical Coherence Tomography Applications
- Molecular Biology Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
Akoya Biosciences (United States)
2019-2024
PerkinElmer (United States)
2012-2017
Health & Life (Taiwan)
2015
Rensselaer Polytechnic Institute
2010
Cabot (United States)
2005-2009
Promega (United States)
2009
National Cancer Institute
2009
Center for Cancer Research
2009
BC Cancer Agency
2008
Novartis (Canada)
2008
<h3>Importance</h3> PD-L1 (programmed cell death ligand 1) immunohistochemistry (IHC), tumor mutational burden (TMB), gene expression profiling (GEP), and multiplex immunohistochemistry/immunofluorescence (mIHC/IF) assays have been used to assess pretreatment tissue predict response anti–PD-1/PD-L1 therapies. However, the relative diagnostic performance of these modalities has yet be established. <h3>Objective</h3> To compare studies that assessed accuracy IHC, TMB, GEP, mIHC/IF in...
The ability to image and quantitate fluorescently labeled markers in vivo has generally been limited by autofluorescence of the tissue. Skin, particular, a strong signal, particularly when excited blue or green wavelengths. Fluorescence labels with emission wavelengths near-infrared are more amenable deep-tissue imaging, because both scattering reduced as increased, but even these spectral regions, can still limit sensitivity. Multispectral imaging (MSI), however, remove signal degradation...
Acousto-optic tunable filters (AOTF) and liquid crystal (LCTF) are evaluated for their suitability as fluorescence microscopy imaging spectrometers. AOTFs solid-state birefringent crystals that provide an electronically spectral notch passband in response to applied acoustic field. LCTFs also a can be controlled by incorporating waveplate retarders within Lyot filter. In this paper, spectroscopic performance quality contrasted evaluation of model systems. Studies include transmission...
Evaluation of T lymphocyte frequency provides prognostic information for patients with oral squamous cell cancer (OSCC). However, the effect simultaneously evaluating and assessing suppressive elements defects in antigen-processing machinery (APM) has not been clarified. Simultaneous characterization CD3+, CD8+, FoxP3+, CD163+, PD-L1+ cells using multispectral imaging was performed on sections from 119 HPV- OSCC. Expression β2-microglobulin, MHC class I heavy chain, large multifunctional...
Adoptive T cell therapy (ACT) has shown great promise in melanoma, with over 50 % response rate patients where autologous tumor-reactive tumor-infiltrating lymphocytes (TIL) can be cultured and expanded. A major limitation of ACT is the inability to generate or expand TIL 25-45 tested. Methods that successfully identify tumors are not suitable for generation by standard methods would eliminate costs fruitless expansion enable these receive alternate immediately.Multispectral fluorescent...
Genomic profiling has identified several molecular oncodrivers in breast tumorigenesis. A thorough understanding of endogenous immune responses to these may provide insights into interventions for cancer (BC). We investigated systemic anti-HER2/neu CD4+ T-helper type-1 (Th1) HER2-driven highly significant stepwise Th1 response loss extending from healthy donors (HD), through HER2pos-DCIS, and ultimately early stage HER2pos-invasive BC patients was detected by IFNγ ELISPOT. The anti-HER2...
A Lyot-type liquid crystal tunable filter (LCTF) suitable for high-definition Raman chemical imaging has been developed. The LCTF incorporated into an efficient system that provides significant performance advantages relative to any previous approach microscopy. and associated optical path is physically compact, which accommodates integration of the within infinity-corrected microscope. simultaneously diffraction-limited spatial resolution 7.6-cm-1 spectral bandpass across full free range...
Combining images taken with light of specific wavelengths can dramatically enhance light-microscopic images. This technology is enabled by the availability programmable filters that be set to transmit only particular wavelengths. Spectral imaging technologies have become an important part microscopy, and are particularly useful for analyzing samples been labeled multiple (two or more) molecular markers. The most commonly used methodology separating markers from each other linear unmixing,...
Abstract Multiplex immunofluorescence (mIF) allows simultaneous antibody-based detection of multiple markers with a nuclear counterstain on single tissue section. Recent studies have demonstrated that mIF is becoming an important tool for immune profiling the tumor microenvironment, further advancing our understanding interplay between cancer and system, identifying predictive biomarkers response to immunotherapy. Expediting discoveries leading improved diagnostic panels, whereas it...
Vertebrate photoreceptors have a modified cilium composed of basal body, axoneme and outer segment. The segment includes stacked membrane discs, containing opsin the signal transduction apparatus mediating phototransduction. In photoreceptors, two distinct classes vesicles are trafficked. Synaptic transported down axon to synapse, whereas opsin-containing continuous replacement segments imposes significant biosynthetic trafficking burden on photoreceptors. Here, we show that <i>Ahi1</i>,...
The dramatic improvement in heat diffusivity of pure copper at liquid helium temperatures makes possible very important advances the absolute accuracy, reproducibility, sensitivity, and time constant cryogenic electrical substitution radiometers (ESRs), relative to conventional ESRs. design characterization a table top ESR now available for detector calibration work 0.01% level accuracy under laser illumination is discussed. A sensitive recently delivered NIST radiometric calibrations black...
We discuss a general technique, laser spectroscopic imaging (LSI), remote acquisition of images biological tissues and tissue conditions. The technique employs laser-induced signals, collected transmitted via an array optical fibers, to produce discrete pixels information from which map or image desired characteristic is constructed. describe prototype LSI catheter that produces spectral the interior human arteries for diagnosis atherosclerosis. diagnostic based on fact normal artery wall...
Continued developments in immuno-oncology require an increased understanding of the mechanisms cancer immunology. The immunoprofiling analysis tissue samples from formalin-fixed, paraffin-embedded (FFPE) biopsies has become a key tool for complexity tumor immunology and discovering novel predictive biomarkers immunotherapy. Immunoprofiling tissues requires evaluation combined markers, including inflammatory cell subpopulations immune checkpoints, microenvironment. advent multiplex...
In vivo fluorescence cancer imaging is an important tool in understanding tumor growth and therapeutic monitoring can be performed either with endogenously produced fluorescent proteins or exogenously introduced probes bound to targeting molecules. However, endogenous cannot altered after transfection, thus requiring rederivation of cell lines for each desired color, while targeted are limited by the heterogeneous expression naturally occurring cellular targets. this study, we adapted...
An active cavity radiometer of the electrical substitution type with a cone receiver that operates at 2-4 K has been developed for measuring radiant fluxes in dynamic range 20 nW to 100 microW within an uncertainty +/-1% (2sigmalevel). It is broadband absolute detector flat overall absorption efficiency >99% radiation from visible long-wavelength IR. The system designed based on thermal modeling and experimental measurements concepts. installed cryogenic chamber low-background infrared...
Two types of electrical substitution radiometers have been developed at Cambridge Research and Instrumentation (CRI) for applications in metrology. We describe a table-top instrument calibrating detectors using stabilized laser radiation, high sensitivity measuring cryogenic black-body an automated system photodiodes thermopiles with several lines radiometer as reference standard. also discuss performance characteristics installed instruments.
Recognizing that guideline-directed histologic grading of endomyocardial biopsy tissue samples for rejection surveillance has limited diagnostic accuracy, quantitative, in situ characterization was performed several important immune cell types a retrospective cohort clinical samples. Differences between cases were identified and grouped by grade versus trajectory, with significantly increased programmed death ligand 1+, forkhead box P3+, cluster differentiation 68+ cells suppressed...
We report on a new class of instrument for imaging spectral analysis, the tunable liquid crystal filter (LCTF). The LCTF is an optical filter, similar to interference whose center wavelength electronically with no moving parts, in few milliseconds, across hundreds nanometers. polarization based Lyot design, using electro-optic action crystals tune passband. Imaging quality near diffraction limit and there image shift as tuned. Bandwidths ranging from 0.25 nm 60 have been achieved, use...
Islet amyloid is hypothesized to play a role in nonimmunologic transplanted islet graft loss. We performed quantitative histologic analysis of liver biopsies from intrahepatic grafts streptozotocin-induced diabetic cynomolgus macaques. Seven animals treated with antithymocyte globulin (ATG) and rapamycin or ATG rituximab experienced rejection lymphocytic infiltrates present on biopsies. Except for one case involving the oldest largest donor where was initial biopsy 1 month after transplant,...
Detection of DNA mutations in tumor tissue can be a critical companion diagnostic test before prescription targeted therapy. Each method for detection these is associated with an analytic sensitivity that function the percentage cells present specimen. Currently, cell visually estimated resulting ordinal and highly variant result biologically continuous variable. We proposed this aspect mutation testing could standardized by developing computer algorithm capable accurately determining...
Abstract Background: Pembrolizumab (Pembro), an anti-PD-1 immune checkpoint inhibitor, has been approved for the treatment of a variety cancers. Pembro was recently evaluated in HER2- breast cancer patients neoadjuvant I-SPY 2 TRIAL and graduated triple negative (TN), HR+HER2-, signatures. TN cancers tend to have high numbers infiltrates, including T cells tumor associated macrophages (TAMs). We utilized multiplex multispectral imaging evaluate presence spatial proximity various cell...