A5059383585- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Viral Infections and Immunology Research
- Cardiac Structural Anomalies and Repair
- Mechanical Circulatory Support Devices
- Organ Transplantation Techniques and Outcomes
- Heart Failure Treatment and Management
- Coronary Interventions and Diagnostics
- Cardiomyopathy and Myosin Studies
- Molecular Biology Techniques and Applications
- Sarcoidosis and Beryllium Toxicity Research
- Cardiovascular Function and Risk Factors
- Atherosclerosis and Cardiovascular Diseases
- COVID-19 diagnosis using AI
- Cardiac electrophysiology and arrhythmias
- Cardiac Imaging and Diagnostics
- Cardiovascular Disease and Adiposity
- Cardiac Valve Diseases and Treatments
- Protease and Inhibitor Mechanisms
- Cardiac pacing and defibrillation studies
- Elasticity and Material Modeling
- Potassium and Related Disorders
- S100 Proteins and Annexins
- Advanced Proteomics Techniques and Applications
- Infective Endocarditis Diagnosis and Management
University of Pennsylvania
2017-2025
Hospital of the University of Pennsylvania
2015-2025
California University of Pennsylvania
2024
Case Western Reserve University
2022
Philadelphia University
2021
Cardiovascular Institute of the South
2021
National Institutes of Health
2006
National Heart Lung and Blood Institute
2003-2005
AIDS Clinical Trials Group
2004
Over 26 million people worldwide suffer from heart failure annually. When the cause of cannot be identified, endomyocardial biopsy (EMB) represents gold-standard for evaluation disease. However, manual EMB interpretation has high inter-rater variability. Deep convolutional neural networks (CNNs) have been successfully applied to detect cancer, diabetic retinopathy, and dermatologic lesions images. In this study, we develop a CNN classifier clinical H&E stained whole-slide images total 209...
New directions in material applications have allowed for the fresh insight into coordination of biophysical cues and regulators. Although role mechanical microenvironment on cell responses mechanics is often studied, most analyses only consider static environments behavior, however, cells tissues are themselves dynamic materials that adapt myriad ways to alterations their environment. Here, we introduce an approach, through addition magnetic inclusions a soft poly(dimethylsiloxane)...
Abstract Aim Allograft rejection is a serious concern in heart transplant medicine. Though endomyocardial biopsy with histological grading the diagnostic standard for rejection, poor inter-pathologist agreement creates significant clinical uncertainty. The aim of this investigation to demonstrate that cellular grades generated via computational analysis are on-par those provided by expert pathologists Methods and results study cohort consisted 2472 slides originating from three major US...
Abstract BACKGROUND Chronic kidney disease (CKD) and arterial stiffness are associated with increased cardiovascular morbidity mortality. Inflammation is proposed to have a role in the development of stiffness, CKD recognized as proinflammatory state. Arterial CKD, cross-sectional data has suggested link between inflammatory markers higher measures stiffness. However, no large scale investigations examined impact inflammation on progression CKD. METHODS We performed baseline assessments 5...
Abstract Aims Right ventricular (RV) failure (RVF) after left assist device (LVAD) implant is an important cause of morbidity and mortality. Modern, data‐driven approaches for defining predicting RVF have been under‐utilized. Methods Two hundred thirty‐two patients were identified with a mean age 55 years; 40 (17%) women, 132 (59%) Caucasian 74 (32%) Black. Patients split between Interagency Registry Mechanically Assisted Circulatory Support (INTERMACS) Classes 1, 2 3 (25%, 38% 34%,...
Background: Cardiac allograft vasculopathy (CAV) is a leading cause of morbidity and mortality for heart transplant recipients. Although clinical risk factors CAV have been established, no personalized prognostic test exists to confidently identify patients at high versus low developing aggressive CAV. This investigation aimed leverage computational methods analyzing digital pathology images from routine endomyocardial biopsies (EMBs) develop precision medicine tool predicting years before...
The XVth Banff Conference on Allograft Pathology meeting was held September 23-27, 2019, in Pittsburgh, Pennsylvania, USA. During this meeting, two main topics cardiac transplant pathology were addressed: (a) Improvement of endomyocardial biopsy (EMB) accuracy for the diagnosis rejection and other significant injury patterns, (b) orphaned lesion known as Quilty effect or nodular endocardial infiltrates. Molecular technologies have evolved recent years, deciphering pathophysiology rejection....
Despite systemic exposure to risk factors, the circulatory system develops varying patterns of atherosclerosis for unclear reasons. In a porcine model, we investigated relationship between site-specific lesion development and inflammatory pathways involved in coronary arteries (CORs) distal abdominal aortas (AAs).Diabetes mellitus (DM) hypercholesterolemia (HC) were induced 37 pigs with 3 healthy controls. Site-specific plaque was studied by comparing severity, macrophage infiltration, gene...
BACKGROUND: Cardiac allograft rejection is the leading cause of early graft failure and a major focus postheart transplant patient care. While histological grading endomyocardial biopsy samples remains diagnostic standard for acute rejection, this has limited accuracy. Discordance between grade clinical trajectory frequently leads to both overtreatment indolent processes delayed treatment aggressive ones, spurring need investigate adequacy current criteria assessing clinically important...
Abstract Background Opportunistic infections (OIs) are a significant cause of morbidity and mortality after organ transplantation, though data in the liver transplant (LT) population limited. Methods We performed retrospective cohort study LT recipients between January 1, 2007 Deceber 31, 2016 using Medicare claims linked to Organ Procurement Transplantation Network database. Multivariable Cox regression models evaluated factors independently associated with hospitalizations for early (≤1...
Background and Aims: While avoidance of long-term corticosteroids is a common objective in the management autoimmune hepatitis (AIH), prolonged immunosuppression usually required to prevent disease progression. This study investigates patient provider factors associated with treatment patterns US patients AIH. Approach Results: A retrospective cohort adults incident prevalent AIH was identified from Optum’s deidentified Clinformatics Data Mart Database. All were followed for at least 2...
Recognizing that guideline-directed histologic grading of endomyocardial biopsy tissue samples for rejection surveillance has limited diagnostic accuracy, quantitative, in situ characterization was performed several important immune cell types a retrospective cohort clinical samples. Differences between cases were identified and grouped by grade versus trajectory, with significantly increased programmed death ligand 1+, forkhead box P3+, cluster differentiation 68+ cells suppressed...
In a rare donation of heart from an individual with osteogenesis imperfecta (OI), we explored cardiomyocyte (CM) adaptations to low stiffness. This represents the first assessment mechanics in OI. The data reveal hypercontractility OI CMs rapid rundown when exposed acute stiffness challenges, extending our understanding These demonstrate that impact on myocardial includes beyond known direct effects extracellular matrix.
Abstract Background The use of induction immunosuppression for heart transplantation (HT) is debated given the uncertain benefit and potential risks infection malignancy. Methods This a retrospective single‐center analysis 475 consecutive HT recipients from 2003 to 2020 grouped by with basiliximab group (BG) no (NBG). Subgroup era compared pre‐2016 standard‐basiliximab (BX) 2016–2020 selective‐BX as part calcineurin‐inhibitor‐sparing regimen. Results When adjusted confounders (sex, age, PRA,...
Cardiac sarcoidosis is poorly understood, challenging to diagnose, and portends a poor prognosis. A lack of animal models necessitates the use residual human samples study sarcoidosis, which in turn analytical tools compatible with archival, fixed tissue. We employed high-plex spatial protein analysis within large cohort archival cardiac control tissue samples, studying immunologic, fibrotic, metabolic landscape at different stages disease, compartments, regions without overt inflammation....