A5002473967- Renal Transplantation Outcomes and Treatments
- Transplantation: Methods and Outcomes
- Renal Diseases and Glomerulopathies
- Organ Transplantation Techniques and Outcomes
- Viral Infections and Immunology Research
- Renal and Vascular Pathologies
- Complement system in diseases
- Organ Donation and Transplantation
- Vasculitis and related conditions
- Neurological Complications and Syndromes
- Cytomegalovirus and herpesvirus research
- Toxin Mechanisms and Immunotoxins
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Pregnancy and preeclampsia studies
- Immune Cell Function and Interaction
- Pediatric Urology and Nephrology Studies
- Immunodeficiency and Autoimmune Disorders
- MicroRNA in disease regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Transgenic Plants and Applications
- Platelet Disorders and Treatments
- HIV/AIDS drug development and treatment
- Eosinophilic Disorders and Syndromes
- Mast cells and histamine
- HIV-related health complications and treatments
Hôpital Necker-Enfants Malades
2016-2025
Assistance Publique – Hôpitaux de Paris
2016-2025
Université Paris Cité
2015-2024
Inserm
2015-2024
Sorbonne Université
1999-2024
Sorbonne Paris Cité
2015-2024
Paris Cardiovascular Research Center
2010-2024
Wayne State University
2024
Michigan United
2024
Institut Necker Enfants Malades
2020-2024
Anti-HLA antibodies hamper successful transplantation, and activation of the complement cascade is involved in antibody-mediated rejection. We investigated whether complement-binding capacity anti-HLA plays a role kidney-allograft failure.We enrolled patients who received kidney allografts at two transplantation centers Paris between January 1, 2005, 2011, population-based study. Patients were screened for presence circulating donor-specific their capacity. Graft injury phenotype time to...
The XV. Banff conference for allograft pathology was held in conjunction with the annual meeting of American Society Histocompatibility and Immunogenetics Pittsburgh, PA (USA) focused on refining recent updates to classification, advances from working groups, standardization molecular diagnostics. This report kidney transplant details clarifications refinements criteria chronic active (CA) T cell–mediated rejection (TCMR), borderline, antibody-mediated (ABMR). main focus sessions how address...
Kidney allograft rejection can occur in clinically stable patients, but long-term significance is unknown. We determined whether early recognition of subclinical has consequences for kidney survival an observational prospective cohort study 1307 consecutive nonselected patients who underwent ABO-compatible, complement-dependent cytotoxicity-negative crossmatch transplantation Paris (2000-2010). Participants screening biopsies at 1 year post-transplant, with concurrent evaluations graft...
Antibodies may have different pathogenicities according to IgG subclass. We investigated the association between subclasses of circulating anti-human HLA antibodies and antibody-mediated kidney allograft injury. Among 635 consecutive transplantations performed 2008 2010, we enrolled 125 patients with donor-specific (DSA) detected in first year post-transplant. assessed DSA characteristics, including specificity, class mean fluorescence intensity (MFI), C1q-binding, subclass, graft injury...
Antibody-mediated rejection (ABMR) can occur in patients with preexisting anti-HLA donor-specific antibodies (DSA) or who develop de novo DSA. However, how these processes compare terms of allograft injury and outcome has not been addressed. From a cohort 771 kidney biopsy specimens from two North American five European centers, we performed systematic assessment clinical biologic parameters, histopathology, circulating DSA, gene expression for all ABMR ( n =205). Overall, 103 (50%) had DSA...
This meeting report from the XV Banff conference describes creation of a multiorgan transplant gene panel by Molecular Diagnostics Working Group (MDWG). Human Organ Transplant (B-HOT) is culmination previous work MDWG to identify broadly useful based on whole transcriptome technology. A data-driven process distilled list peer-reviewed comprehensive microarray studies that discovered and validated their use in kidney, liver, heart, lung biopsies. These were supplemented genes define relevant...
Abstract In kidney transplantation, day-zero biopsies are used to assess organ quality and discriminate between donor-inherited lesions those acquired post-transplantation. However, many centers do not perform such since they invasive, costly may delay the transplant procedure. We aim generate a non-invasive virtual biopsy system using routinely collected donor parameters. Using 14,032 from 17 international centers, we develop system. 11 basic parameters predict four Banff lesions:...
The significance of C4d-Banff scores in protocol biopsies kidney transplant recipients with preformed donor-specific antibodies (DSA) has not been determined. We reviewed 157 from 80 DSA+ patients obtained at 3 months and 1 year post-transplant. C4d Banff (1,2,3) were associated significant increments microcirculation inflammation (MI) both post-transplant, worse glomerulopathy higher class II DSA-MFI (p < 0.01). Minimal-C4d had injury intermediate between negative focal, while focal...
Sirolimus has been associated with high-range proteinuria when used in replacement of calcineurin inhibitors renal transplant recipients chronic allograft nephropathy (CAN). Primary FSGS was demonstrated previously some such patients, but the coexistence CAN lesions made interpretation uneasy. However, nephrotic syndrome and were observed recently three patients who received sirolimus de novo, without medical history primary or CAN. Markers podocyte differentiation studied kidney biopsies...
Antibody-mediated rejection (ABMR) is the leading cause of kidney allograft loss. We investigated whether addition gene expression measurements to conventional methods could serve as a molecular microscope identify kidneys with ABMR that are at high risk for failure. studied 939 consecutive recipients Necker Hospital (2004-2010; principal cohort) and 321 Saint Louis (2006-2010; validation assessed patients in first 1 year post-transplant. In features, we microarray-based transplant biopsy...
Thrombotic microangiopathy (TMA) occurs in IgA nephropathy, but its clinical significance is not well described. We retrospectively examined a series of 128 patients diagnosed with nephropathy between 2002 and 2008 who had mean follow-up 44±27 months. In our series, 53% presented lesions TMA, acute or organized, arteries and/or arterioles. Among 4% were normotensive, 25% controlled hypertension, 71% uncontrolled hypertension. Of those 26% malignant Histologically, the group TMA significantly...
Urinary levels of C-X-C motif chemokine 9 (CXCL9) and CXCL10 can noninvasively diagnose T cell-mediated rejection (TCMR) renal allografts. However, performance these molecules as diagnostic/prognostic markers antibody-mediated (ABMR) is unknown. We investigated urinary CXCL9 in a highly sensitized cohort 244 allograft recipients (67 with preformed donor-specific antibodies [DSAs]) 281 indication biopsy samples. assessed the benefit adding biomarkers to conventional models for...
Several different entities have recently been described among glomerular diseases associated with monoclonal IgG deposits. The aim of this study was to describe the distribution pathologic subtypes IgG-associated glomerulopathy and evaluate isotype involved in these diseases.This a retrospective including all patients deposits referred three nephrology departments between 1980 2008.Twenty-six were included. Nephrotic syndrome almost constantly renal dysfunction 14 26 patients. presence...
The diagnosis system for allograft loss lacks accurate individual risk stratification on the basis of donor–specific anti–HLA antibody (anti-HLA DSA) characterization. We investigated whether systematic monitoring DSA with extensive characterization increases performance in predicting kidney loss. This prospective study included 851 recipients transplanted between 2008 and 2010 who were systematically screened at transplant, 1 2 years post-transplant, time post–transplant clinical events....
Export Complement-activating anti-HLA donor-specific antibodies (DSAs) are associated with impaired kidney transplant outcome; however, whether these induce a specific rejection phenotype and influence response to therapy remains undetermined. We prospectively screened 931 recipients for complement-activating DSAs used histopathology, immunostaining, allograft gene expression assess phenotypes. Effector cells were evaluated using in vitro human cell cultures. Additionally, we assessed the...
The recent recognition of complex and chronic phenotypes T cell-mediated rejection (TCMR) has fostered the need to better evaluate response acute TCMR-a condition previously considered lack relevant consequences for allograft survival-to standard care. In a prospective cohort kidney recipients (n = 256) with biopsy-proven TCMR receiving corticosteroids, we investigated clinical, histological, immunological at time diagnosis 3 months posttreatment. Independent posttreatment determinants loss...
Cardiac allograft vasculopathy (CAV) is a major contributor of heart transplant recipient mortality. Little known about the prototypes CAV trajectories at population level. We aimed to identify different evolutionary profiles and determine respective contribution immune nonimmune factors in development.Heart recipients were from 4 academic centers (Pitié-Salpêtrière Georges Pompidou Hospital, Paris, Katholieke Universiteit Leuven, Cedars-Sinai, Los Angeles; 2004-2016). Patients underwent...
Alplesib exerts therapeutic effects in a mouse model of lymphatic anomalies and six patients with malformations over 6 months’ treatment.
De novo thrombotic microangiopathy (dnTMA), after renal transplantation may significantly alter graft outcomes. However, its pathogenesis and the role of complement alternative pathway dysregulation remain elusive. We studied all consecutive adult patients with a kidney allograft biopsy performed between January 2004 March 2016 displaying dnTMA. Ninety-two were included. The median time occurrence was 166 (IQR 25-811) days. majority (82.6 %) had TMA localized only in graft. Calcineurin...
The multidrug resistance-associated protein (MRP) that is involved in drug resistance and the export of glutathione-conjugated substrates may not have same epithelial cell membrane distribution as P-glycoprotein encoded by MDR gene. Because intestinal kidney cells are polarized endowed distinct secreting absorptive ion transport capacities, we investigated tissue MRP adult mouse small intestine, colon, immunohistochemistry. Western blot analyses revealed 190-kD these tissues. was found...