A5025333280- Renal Transplantation Outcomes and Treatments
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Organ Donation and Transplantation
- Renal Diseases and Glomerulopathies
- Anesthesia and Sedative Agents
- Renal and Vascular Pathologies
- Airway Management and Intubation Techniques
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Medical Imaging and Pathology Studies
- Organ Transplantation Techniques and Outcomes
- Blood groups and transfusion
- Hematopoietic Stem Cell Transplantation
- Vasculitis and related conditions
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Intensive Care Unit Cognitive Disorders
- Respiratory Support and Mechanisms
- Anesthesia and Neurotoxicity Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immunotherapy and Immune Responses
- Anesthesia and Pain Management
- Lung Cancer Diagnosis and Treatment
- Organ and Tissue Transplantation Research
- Cell Image Analysis Techniques
Arkana Laboratories
2023-2024
Columbia University Irving Medical Center
2014-2024
Columbia University
2016-2022
Presbyterian Hospital
2021
New York Hospital Queens
2012-2021
NewYork–Presbyterian Hospital
2012-2021
Queen Elizabeth University Hospital
2017-2018
University of Glasgow
2016-2018
Mosaiques Diagnostics and Therapeutics (Germany)
2016
Cleveland Clinic
2014
Abstract In kidney transplantation, day-zero biopsies are used to assess organ quality and discriminate between donor-inherited lesions those acquired post-transplantation. However, many centers do not perform such since they invasive, costly may delay the transplant procedure. We aim generate a non-invasive virtual biopsy system using routinely collected donor parameters. Using 14,032 from 17 international centers, we develop system. 11 basic parameters predict four Banff lesions:...
Abstract Proliferative renewal of memory CD8 T cells is essential for maintaining long-term immunity. In this study, we examined the contributions that various tissue microenvironments make toward homeostatic proliferation Ag-specific cells. We found dividing were present in both lymphoid and nonlymphoid tissues. However, bone marrow was preferred site contained a major pool most actively Adoptive transfer studies indicated migrated through divided there preferentially. These results show...
A paradigm in transplantation states that graft-infiltrating T cells are largely non-alloreactive "bystander" cells. However, the origin and specificity of allograft over time has not been investigated detail animals or humans. Here, we use polychromatic flow cytometry high throughput TCR sequencing serial biopsies to show gut-resident cell turnover kinetics human intestinal allografts correlated with balance between intra-graft host-vs-graft (HvG) graft-vs-host (GvH) reactivities clinical...
BACKGROUND Increasingly, minimally invasive procedures are performed to assess lung lesions and stage carcinomas. In cases of advanced‐stage cancer, the biopsy may provide only diagnostic tissue. The aim this study was determine which method—fine‐needle aspiration (FNA), core (CBx), or both (B)—is optimal for providing sufficient tissue rendering a specific diagnosis pursuing molecular studies guiding tumor‐specific treatment. METHODS A search computed tomography–guided FNA, CBx, B with...
The XVI-th Banff Meeting for Allograft Pathology was held in Banff, Alberta, Canada, from 19th-23rd September 2022, as a joint meeting with the Canadian Society of Transplantation. In addition to key focus on impact microvascular inflammation and biopsy-based transcript analysis Classification, further sessions were devoted other aspects kidney transplant pathology, particular T cell-mediated rejection, activity chronicity indices, digital xenotransplantation, clinical trials, surrogate...
OBJECTIVE--To see whether parathyroid hormone related protein has a humoral role in breast cancer. DESIGN--Plasma concentrations and tumour expression of were determined (by two site immunoradiometric assay immunohistochemistry respectively) women with cancer to the presence bone metastases serum calcium concentrations. SUBJECTS--Plasma measured 57 early without apparent metastases, 28 13 hypercalcaemia. Tissue positivity for was retrospectively 106 primary tumours from 72 25 whom...
Although T-cell CD28/CD40 costimulation blockade represents a powerful mechanism to promote immune tolerance during murine allotransplantation, it has not yet been successfully translated clinical transplantation. We determined the impact of natural killer (NK) cells on blockade-resistant rejection donor bone marrow. found that NK represent potent barrier engraftment: host depletion led increased stem cell survival, mixed hematopoietic chimerism and engraftment low doses marrow (1 x...
Background and objectives Unfavorable histology on procurement biopsies is the most common reason for deceased donor kidney discard. We sought to assess reproducibility of biopsy findings. Design, setting, participants, & measurements compiled a continuous cohort kidneys transplanted at our institution from 1/1/2006 12/31/2016 that had least one performed, excluded cases with missing reports those used in multiorgan transplants. Suboptimal was defined as presence advanced sclerosis...
Summary The analgesic efficacy of subcutaneous wound infiltration with 20 ml 0.5% bupivacaine after elective lower segment section Caesarean was studied in 28 patients a double‐blind randomised controlled manner using patient‐controlled analgesia system. mean 24‐hour morphine consumption the placebo group and similar (76 mg 68 respectively). Analysis cumulative hourly failed to show any statistically significant differences between groups. However, on weight‐adjusted basis were demonstrated,...
An 11-year retrospective review of women with breast abscesses presenting to a district general hospital was performed. A total 122 were identified abscess: 85 (70 per cent) non-lactational and 37 (30 an abscess in the puerperium. Six lactational 24 recurred. Sixteen mammillary fistulae developed, all following incision drainage abscesses. Women more likely smoke cigarettes (P less than 0.005). Breast containing anaerobic bacteria significantly occur current cigarette smokers 0.05). who...
Abstract Chronic kidney disease and associated comorbidities (diabetes, cardiovascular diseases) manifest with an accelerated ageing phenotype, leading ultimately to organ failure renal replacement therapy. This process can be modulated by epigenetic environmental factors which promote loss of physiological function resilience stress earlier, linking biological age adverse outcomes post‐transplantation including delayed graft (DGF). The molecular features underpinning this have yet fully...
The factors that influence deceased donor kidney procurement biopsy reliability are not well established. We examined the impact of technique and pathologist training on accuracy.We retrospectively identified all kidney-only transplants at our center from 2006 to 2016 with both reperfusion biopsies performed information available (n = 392). Biopsies were scored using a previously validated system, classifying "suboptimal" histology as presence least 1 following: glomerulosclerosis ≥11%,...
Treatment with immune checkpoint blockade (ICB) often fails to elicit durable antitumor immunity. Recent studies suggest that ICB does not restore potency terminally dysfunctional T cells, but instead drives proliferation and differentiation of self-renewing progenitor cells into fresh, effector-like cells. Antitumor immunity catalyzed by is characterized mobilization in systemic circulation tumor. To address whether abundance blood associated immunotherapy response, we used flow cytometry...
Background Immune checkpoint blockade has emerged as a highly effective treatment for patients with metastatic melanoma and cutaneous squamous cell carcinoma. Nivolumab blocks the interactions between programmed death protein 1 ligand allowing activation of latent immune response against malignancy. Ipilimumab binds to cytotoxic T-lymphocyte-associated 4, alleviating negative regulation T-cell that is mediated by checkpoint. Combination therapy nivolumab ipilimumab associated longer overall...
CD8 T cells are necessary for costimulation blockade-resistant rejection. However, the mechanism by which mediate rejection in absence of major costimulatory signals is poorly understood. IFN-gamma promotes cell-mediated immune responses, but IFN-gamma-deficient mice show early graft loss despite blockade. In contrast, we found that receptor knockout dramatically prolonged survival under To investigate this paradox, addressed effects on cell alloresponses vivo independent survival. We...
Fine-needle aspirations (FNAs) and core biopsies (CBs), with or without touch preparations (TPs), are performed to characterize pulmonary lesions. Although a positive (P) suspicious report is sufficient for further management, the significance of unsatisfactory (U), negative (N) atypical (A) cytological diagnoses remains uncertain. The aims study were correlate U, N A histological and/or clinical/radiological follow-up evaluate utility FNAs, TPs CBs.We retrospective search examined 30...
The XVI-th Banff Meeting for Allograft pathology was held in Canada, from 19th-23rd September 2022, as a joint meeting with the Canadian Society of Transplantation. In addition to key focus on microvascular inflammation and biopsy-based transcript analysis Classification, further sessions were devoted other aspects transplant pathology, particular T cell-mediated rejection, activity chronicity indices, digital xenotransplantation, clinical trials, surrogate endpoints. Although output these...
Gene expression profiling (GEP) from formalin-fixed paraffin-embedded (FFPE) renal allograft biopsies is a promising approach for feasibly providing molecular diagnosis of rejection. However, large-scale studies evaluating the performance models using NanoString platform data to define archetypes rejection are lacking. We tested diverse retrospective cohort over 1400 FFPE biopsy specimens, rescored according Banff 2019 criteria and representing ten 11 UNOS regions, Human Organ Transplant...