While optical coherence tomography (OCT) provides a resolution down to 1 µm, it has difficulties in visualizing cellular structures due lack of scattering contrast. By evaluating signal fluctuations, significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes and subcellular visible. The putative cause the dynamic is site-dependent active motion sub-micrometer range, histology-like Here we demonstrate with scanning frequency-domain (FD-OCT),...

Significance: Selective cryolipolysis is a widely used aesthetic procedure that cools subcutaneous adipose tissue to temperatures as low −11°C induce fat cell destruction. However, real-time monitoring techniques are lacking, limiting the ability optimize safety and efficacy. Traditional imaging methods either fail provide adequate penetration depth or lack resolution necessary for visualizing fatty dynamics.Aim: This paper aims demonstrate an optical coherence tomography (OCT) needle probe...

Optical coherence tomography (OCT) enables the non-invasive acquisition of high-resolution three-dimensional cross-sectional images at micrometer scale and is mainly used in field ophthalmology for diagnosis as well monitoring eye diseases. Also other areas, such dermatology, OCT already established. Due to its nature, also employed research studies involving animal models. Manual evaluation models a challenging task due lack imaging standards varying anatomy among In this paper, we present...

Fluorescence lifetime imaging microscopy (FLIM) of intrinsic fluorophores such as nicotinamide adenine dinucleotide (NADH) allows for label-free quantification metabolic activity individual cells over time and in response to various stimuli, which is not feasible using traditional methods due their destructive nature lack spatial information. This study uses FLIM measure pharmacologically induced changes that occur during the browning white fat. Adipocyte increases energy expenditure, making...

Background and Objectives A recent generation of 5,500 nm wavelength carbon monoxide (CO) lasers could serve as a novel tool for applications in medicine surgery. At this wavelength, the optical penetration depth is about three times higher than that 10,600 dioxide (CO 2 ) laser. As amount ablation coagulation strongly influenced by we anticipated CO would provide extended zones, which be beneficial several medical applications, such tissue tightening effects after laser skin resurfacing....

Abstract Visualization and quantification of the skin microvasculature are important for studying health human microcirculation. We correlated structural pathophysiological changes dermal capillary-level with age blood pressure by using reactive hyperemia optical coherence tomography angiography (RH-OCT-A) technique evaluated both conventional OCT-A RH-OCT-A method as non-invasive imaging alternatives to histopathology. This observational pilot study acquired images 13 young 12 old healthy...

Optical coherence tomography angiography (OCTA) provides in-vivo images of microvascular perfusion in high resolution. For its application to basic and clinical research, an automatic robust quantification the capillary architecture is mandatory. Only this makes it possible reliably analyze large amounts image data, establish biomarkers, monitor disease developments. However, due optical properties, OCTA skin often suffer from a poor signal-to-noise ratio contain imaging artifacts. Previous...

Background Traditionally, fractional laser treatments are performed with focused sources operating at a fixed wavelength. Using tunable in the mid‐infrared wavelength range, wavelength‐dependent absorption properties on ablation process and thermal damage formation were assessed goal to obtain customizable tissue ablations provide guidance finding optimized exposure parameters for clinical applications. Methods Laser experiments carried out full thickness ex vivo human abdominal skin using...

Background/Objectives Standard optical coherence tomography angiography (OCTA) has been limited to imaging blood vessels actively undergoing perfusion, providing a temporary picture of surface microvasculature. Capillary perfusion in the skin is dynamic and changes response surrounding tissue's respiratory, nutritional, thermoregulatory needs. Hence, OCTA often represents given state without depicting actual extent vascular network. Here we present method for obtaining more accurate anatomic...

To improve particle radiotherapy, we need a better understanding of the biology radiation effects, particularly in heavy ion therapy, where global responses are observed despite energy deposition only subset cells. Here, integrated high-speed swept confocally-aligned planar excitation (SCAPE) microscope into focused beam irradiation platform to allow real-time 3D structural and functional imaging living biological samples during after irradiation. We demonstrate dynamic acute effects on...

Cryolipolysis is a well-established cosmetic procedure for non-invasive local fat reduction. This technique selectively destroys subcutaneous cells using controlled cooling. Thickness measurements of were conducted mouse model. For detailed examination skin optical coherence tomography (OCT) was performed, which imaging modality. Due to high number image slices manual delineation not feasible. Therefore, automatic segmentation algorithms are required. In this work an algorithm the 3D layer...

Cryolipolysis is a noninvasive method of destroying adipocytes using controlled cooling, thereby enabling localized and targeted fat reduction. Due to their greater vulnerability cold injury, are selectively targeted, while other cell types spared.This study aims develop mouse model cryolipolysis offer reliable convenient alternative human models, providing methodology validate clinical hypotheses in-depth with relative ease, low cost, efficiency. This further facilitates comprehensive...

To improve particle radiotherapy, we need a better understanding of the biology targeted and non-targeted radiation effects. This is particularly important in field heavy ion therapy where global effects are observed, yet only subset cells receive high energy deposition from tracks. allow real-time 3D imaging living biological samples during after irradiation with focused beam, have integrated high-speed light sheet fluorescence system (swept confocally aligned planar excitation, SCAPE...

To improve particle radiotherapy, we need a better understanding of the biology targeted and non-targeted radiation effects. This is particularly important in field heavy ion therapy where global effects are observed, yet only subset cells receive high energy deposition from tracks. allow real-time 3D imaging living biological samples during after irradiation with focused beam, have integrated high-speed light sheet fluorescence system (swept confocally aligned planar excitation, SCAPE...

In dermatology the reflexes of vasoconstriction and vasodilation are known as important mechanisms thermoregulation inner body. Imaging physiology microvasculature skin with high spatial resolution in three dimensions while reacting to changes temperature is crucial for understanding complex processes vasodynamics, which result constriction dilation vessels. However, previous studies using Laser-Doppler flowmetry -imaging could not provide reliable angiographic images allow quantify blood...

The ability to image the physiology of microvasculature with high spatial resolution in three dimensions while investigating structural changes skin, is essential for understanding complex processes skin aging, wound healing and disease development. Further, quantitative, automatic assessment these enables analyze large amounts data an abstract but comprehensive manner. However, previous work using OCT methods angiography was imaging less scattering, hence more challenging tissue than such...

The evaluation of complex metabolic changes individual live cells and heterogeneous cell cultures is not feasible using traditional methods due to their destructive behavior lack spatial information. Two-photon excited fluorescence intrinsic fluorophores such as nicotinamide adenine dinucleotide (NADH) facilitate a label-free non-destructive activity. This study explores the phasor approach in combination with two-photon lifetime imaging microscopy (FLIM) potential method evaluate...

Cryolipolysis has become a popular non-invasive method of reducing excess fat by cooling. However, the results vary as metabolism, gender and diet may affect freezing point human subcutaneous fat. To increase success for all patients, it is essential to better understand process cryolipolysis in vivo. Therefore, we have developed side-facing needle probe with an outer diameter 390 µm achieving lateral resolution 10 at working distance 1.5 mm. obtain spatially resolved visualization immediate...

Optical coherence tomography angiography (OCTA) provides in-vivo images of microvasculature. In skin it often represents a dynamic perfusion state without depicting the actual extent vascular network. Here, we present capillary refill method for obtaining more accurate anatomic representation surface networks in human using OCTA. OCTA were captured at baseline displaying ambient and after compression release representing network existing capillaries full capacity. This mapping cutaneous...

While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties visualize cellular structures due lack of scattering contrast. By evaluating signal fluctuations, significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes and subcellular visible. The putative cause the dynamic is ATP-dependent motion in sub-micrometer range, histology-like Here we demonstrate with scanning frequency-domain (FD-OCT). Given...