A5003395622- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Olfactory and Sensory Function Studies
- Neural dynamics and brain function
- Cell Image Analysis Techniques
- Radiation Therapy and Dosimetry
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Advanced Electron Microscopy Techniques and Applications
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Virus-based gene therapy research
- Orbital Angular Momentum in Optics
- Cold Atom Physics and Bose-Einstein Condensates
- Advanced Vision and Imaging
- Cancer Research and Treatments
- Quantum many-body systems
- Digital Holography and Microscopy
- Microfluidic and Bio-sensing Technologies
- Sperm and Testicular Function
- Strong Light-Matter Interactions
- Optical Imaging and Spectroscopy Techniques
Columbia University
2017-2024
Brain (Germany)
2024
Optica
2018-2023
Laboratoire d’Imagerie Biomédicale
2023
Max Planck Society
2011-2013
Max Planck Institute of Molecular Cell Biology and Genetics
2011-2013
Universidad Nacional Autónoma de México
2009
The ever-increasing speed and resolution of modern microscopes make the storage post-processing images challenging prevent thorough statistical analyses in developmental biology. Here, instead deploying massive computing power, we exploit spherical geometry zebrafish embryos by a radial maximum intensity projection real time with 240-fold reduction data rate. In our four-lens selective plane illumination microscope (SPIM) setup development multiple is recorded parallel map all labelled cells...
Neurophotonics was launched in 2014 coinciding with the launch of BRAIN Initiative focused on development technologies for advancement neuroscience. For last seven years, Neurophotonics' agenda has been well aligned this focus neurotechnologies featuring new optical methods and tools applicable to brain studies. While 2.0 is pivoting towards applications these novel quest understand brain, article we review an extensive diverse toolkit explore function that have emerged from related...
Across the nervous system, neurons with similar attributes are topographically organized. This topography reflects developmental pressures. Oddly, vestibular (balance) nuclei thought to be disorganized. By measuring activity in birthdated neurons, we revealed a functional map within central projection nucleus that stabilizes gaze larval zebrafish. We first discovered both somatic position and stimulus selectivity follow neuron birthdate. Next, electron microscopy loss-of-function assays,...
For optical tweezers, especially when used in biological studies, optimizing the trapping efficiency reduces photo damage or enables generation of larger forces. One important, yet not-well understood, tuning parameter is how much laser beam needs to be expanded before coupling it into objective. Here, we measured trap stiffness for 0.5-2 μm-diameter microspheres various expansions. We show that highest overall achieved by slightly under-filling a high-numerical aperture objective using with...
Learning alters cortical representations and improves perception. Apical tuft dendrites in layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated...
SUMMARY Learning alters cortical representations and improves perception. Apical tuft dendrites in Layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not...
ABSTRACT Across the nervous system, neurons with similar attributes are topographically organized. This topography reflects developmental pressures. Oddly, vestibular (balance) nuclei thought to be disorganized. By measuring activity in birthdated neurons, we revealed a functional map within central projection nucleus that stabilizes gaze larval zebrafish. We first discovered both somatic position and stimulus selectivity follow neuron birthdate. Next, electron microscopy loss-of-function...
To improve particle radiotherapy, we need a better understanding of the biology radiation effects, particularly in heavy ion therapy, where global responses are observed despite energy deposition only subset cells. Here, integrated high-speed swept confocally-aligned planar excitation (SCAPE) microscope into focused beam irradiation platform to allow real-time 3D structural and functional imaging living biological samples during after irradiation. We demonstrate dynamic acute effects on...
Learning alters cortical representations and improves perception. Apical tuft dendrites in Layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated stimulus...
Learning alters cortical representations and improves perception. Apical tuft dendrites in Layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated...
Learning alters cortical representations and improves perception. Apical tuft dendrites in layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated...
SCAPE microscopy allows real-time 3D visualization of intact human tissues at cellular resolution using autofluorescence or topical/intravenous dyes. We are developing a portable clinical system suitable for open/laparoscopic surgery and surveillance accessible tissues.