A5044296947- Microbial infections and disease research
- Viral gastroenteritis research and epidemiology
- Random lasers and scattering media
- Aquaculture disease management and microbiota
- Immune Response and Inflammation
- Advanced Fluorescence Microscopy Techniques
- Salmonella and Campylobacter epidemiology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Immune cells in cancer
- Escherichia coli research studies
- Bacterial Identification and Susceptibility Testing
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- Hemodynamic Monitoring and Therapy
- Cardiovascular Health and Disease Prevention
- Reproductive Physiology in Livestock
- Peroxisome Proliferator-Activated Receptors
- Mycobacterium research and diagnosis
- Aquaculture Nutrition and Growth
- Animal Virus Infections Studies
- Atomic and Subatomic Physics Research
- Adenosine and Purinergic Signaling
- Tissue Engineering and Regenerative Medicine
- Orbital Angular Momentum in Optics
- Reproductive System and Pregnancy
Czech Academy of Sciences, Institute of Scientific Instruments
2021-2023
Veterinary Research Institute
2012-2022
Masaryk University
2012
Abstract Light-based in-vivo brain imaging relies on light transport over large distances of highly scattering tissues. Scattering gradually reduces contrast and resolution, making it difficult to reach structures at greater depths even with the use multiphoton techniques. To deeper, minimally invasive endo-microscopy techniques have been established. These most commonly exploit graded-index rod lenses enable a variety modalities in head-fixed freely moving animals. A recently proposed...
Neurophotonics was launched in 2014 coinciding with the launch of BRAIN Initiative focused on development technologies for advancement neuroscience. For last seven years, Neurophotonics' agenda has been well aligned this focus neurotechnologies featuring new optical methods and tools applicable to brain studies. While 2.0 is pivoting towards applications these novel quest understand brain, article we review an extensive diverse toolkit explore function that have emerged from related...
Abstract Genes localized at Salmonella pathogenicity island-1 (SPI-1) are involved in enterica invasion of host non-professional phagocytes. Interestingly, macrophages, SPI-1-encoded proteins, addition to invasion, induce cell death via activation caspase-1 which also cleaves proIL-1β and proIL-18, precursors 2 proinflammatory cytokines. In this study we were therefore interested whether type III secretion system (T3SS) may influence response macrophages. To test hypothesis, infected primary...
Multimode fiber-based endoscopes have recently emerged as a tool for minimally invasive endoscopy in tissue, at depths well beyond the reach of multiphoton imaging. Here, we demonstrate label-free second-harmonic generation (SHG) microscopy through such fiber endoscope. We simultaneously fully control excitation polarization state and spatial distribution light tip, use this to implement polarization-resolved SHG imaging, which allows imaging identification structural proteins collagen...
Abstract Background Myosatellite cells are myogenic stem that can transform to provide nuclei for existing muscles or generate new muscle fibers as documented after extended exercise programs. Objectives The authors investigated whether the simultaneous application of High-Intensity Focused Electromagnetic (HIFEM) and Synchrode radiofrequency (RF) affects levels satellite similarly prolonged does achieve growth. Methods Three 30-minute HIFEM RF treatments (once a week) were administered over...
Abstract Background In this study we were interested in the colonisation and early immune response of Balb/C mice to infection with Salmonella Enteritidis isogenic pathogenicity island free mutants. Results The virulence S . for was exclusively dependent on intact SPI-2. Infections any mutants harbouring SPI-2 (including mutant which left only but removed SPI-1, SPI-3, SPI-4 SPI-5) resulted fatalities, liver injures NK cell depletion from spleen. minimal influence counts splenic CD4 CD8 T...
The goals of our study were to compare the immune response different killed and modified live vaccines against PRRS virus monitor antibody production cell mediated immunity both at systemic local level. In experiment, we immunized four groups piglets with two commercial inactivated (A1 – Progressis, A2 Suivac) (B3 Amervac, B4 Porcilis). Twenty-one days after final vaccination, all piglets, including control non-immunized group (C5), i.n. infected Lelystad strain virus. serum (IgM IgG) was...
Abstract Monocytes play an essential role in the defense against bacterial pathogens. Bone marrow (BM) and peripheral blood (PB) monocytes pigs consist of main “steady-state” subpopulations: CD14 hi /CD163 - /SLA-DR low + . During inflammation, subpopulation “inflammatory” expressing very high levels CD163, but lacking SLA-DR molecule (being ) appears BM PB replaces subpopulation. However, current knowledge monocyte migration into inflamed tissues is limited. The aim present study was to...
Microendoscopes based on optical fibres have recently come to the fore as promising candidates allowing in-vivo observations of otherwise inaccessible biological structures in animal models. Despite being still its infancy, imaging can now be performed at tip a single multimode fibre, by relying powerful holographic methods for light control. Fibre endoscopy is commonly en face , resulting possible damage specimen owing direct contact between distal end probe and target. On this ground, we...
Abstract Encephalitozoonosis is a common infectious disease widely spread among rabbits. Encephalitozoon cuniculi , considered as zoonotic and emerging pathogen capable of infecting both immunocompetent immunocompromised hosts. The aim the study was to describe in detail E. rabbit organism after experimental infection host humoral cellular immune response including cytokine production. For that purpose, healthy rabbits were infected orally order simulate natural route euthanised at 2, 4, 6...
To date, only limited information about the influence of ovarian hormones on canine immune system cells has been available. The study investigated in vitro progesterone and estradiol-17β activity neutrophils. Treatment by both led to a significant decrease phagocytosis-induced oxidative burst as detected using luminometry after stimulation with opsonised zymosan. increase burst, not connected phagocytosis, was recorded soluble stimulator. Using flow cytometry, tendency production reactive...
Salmonella enterica serovar Typhimurium and Derby are the most common serovars of ssp. found in pigs Europe. We previously observed that suckling piglets sows vaccinated with an S. Typhimurium-based inactivated vaccine protected against homologous strain challenge. To develop this for commercial use, potential crossprotectivity to challenge was tested. Two were while two other remained serologically negative. Four-day-old from both groups orally challenged or Typhimurium. Maternally-derived...
Respiratory infections are a real threat for humans, and therefore the pig model is of interest studies. As one case studies, Actinobacillus pleuropneumoniae (APP) caused still worries many breeders around world. To better understand influence pathogenic effect APP on respiratory system—lungs tracheobronchial lymph nodes (TBLN), we aimed to employ matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI). In this study, six pigs were intranasally...
Proper histological evaluation of lung tissue and accurate antigen detection by immunodetection techniques require processing to preserve reactivity open alveolar spaces. In this study, we focused on testing comparing different procedures cryopreservation. Sucrose or Tissue Tek were used with several methods freezing samples supercooled liquids liquid nitrogen. Changes in caused during the effect cryoprotectants recorded. Rat porcine pulmonary tissues experiment. This study aimed optimize...
Fish are exposed to numerous stressors in the environment including pollution, bacterial and viral agents, toxic substances. Our study with common carps leveraged an integrated approach (i.e., histology, biochemical hematological measurements, analytical chemistry) understand how cyanobacteria interfere impact of a model agent, Carp sprivivirus (SVCV), on fish. In addition specific effects single stressor (SVCV or cyanobacteria), combination both worsens markers related immune system liver...