A5080367058- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- DNA Repair Mechanisms
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Multiple Myeloma Research and Treatments
- Advanced Fluorescence Microscopy Techniques
- Cardiovascular Health and Disease Prevention
- bioluminescence and chemiluminescence research
- Electromagnetic Fields and Biological Effects
- Cancer-related gene regulation
- Hemodynamic Monitoring and Therapy
- Magnetic and Electromagnetic Effects
- Protein Degradation and Inhibitors
- Atomic and Subatomic Physics Research
- Glycosylation and Glycoproteins Research
- Optical Imaging and Spectroscopy Techniques
- Sulfur Compounds in Biology
- Ear and Head Tumors
- Chronic Lymphocytic Leukemia Research
Czech Academy of Sciences, Institute of Biophysics
2014-2024
Czech Academy of Sciences
2006-2022
In-Q-Tel
2007
Abstract Light-based in-vivo brain imaging relies on light transport over large distances of highly scattering tissues. Scattering gradually reduces contrast and resolution, making it difficult to reach structures at greater depths even with the use multiphoton techniques. To deeper, minimally invasive endo-microscopy techniques have been established. These most commonly exploit graded-index rod lenses enable a variety modalities in head-fixed freely moving animals. A recently proposed...
Although histone acetylation is one of the most widely studied epigenetic modifications, there still a lack information regarding how acetylome regulated during brain development and pathophysiological processes. We demonstrate that embryonic (E15) characterized by an increase in H3K9 as well decreases levels HDAC1 HDAC3. Moreover, experimental induction hyperacetylation led to overexpression NCAM cortex depletion Sox2 subventricular ependyma, which mimicked differentiation Inducing...
Abstract Epigenetic marks are important factors regulating the pluripotency and differentiation of human embryonic stem cells (hESCs). In this study, we analyzed H3K9 acetylation, an epigenetic mark associated with transcriptionally active chromatin, during endoderm‐like hESCs. ChIP‐on‐chip analysis revealed that results in a genome‐wide decrease promoter acetylation. Among 24,659 promoters analyzed, only 117 likely to be involved pluripotency, while 25 acetylated responsible for...
Abstract Epigenetic histone (H3) modification patterns and the nuclear radial arrangement of select genetic elements were compared in human embryonic stem cells (hESCs) before after differentiation. H3K9 acetylation, trimethylation, H3K79 monomethylation reduced at periphery differentiated hESCs. Differentiation coincided with centromere redistribution, as evidenced by perinucleolar accumulation centromeric markers CENP‐A H3K9me3, central repositioning centromeres 1, 5, 19, rearrangement...
Methylation of histones H4 at lysine 20 position (H4K20me), which is functional in DNA repair, represents a binding site for the 53BP1 protein. Here, we show radiation-induced increase level H4K20me3 while levels H4K20me1 and H4K20me2 remained intact. was significantly pronounced lesions only G1 phase cycle, this histone mark reduced very late S G2 phases when PCNA recruited to locally micro-irradiated chromatin. diminished irradiated Suv39h1/h2 double knockout (dn) fibroblasts, same...
Abstract Histone variants and their epigenetic modifications determine genome function, particularly transcription. However, whether regulation of gene expression can be influenced by nuclear organization or vice versa is not completely clear. Here, we analyzed the effect changes induced a histone deacetylase inhibitor (HDACi) on radial rearrangement select genomic regions chromosomes. The HDACi, sodium butyrate (NaBt), differentiation human adenocarcinoma HT29 cells as well genome‐wide...
ABSTRACT We examined the levels and distribution of post‐translationally modified histones protamines in human sperm. Using western blot immunoassay, immunofluorescence, mass spectrometry (MS), FLIM‐FRET approaches, we analyzed status histone modifications protamine P2. Among individual samples, observed variability H3K9me1, H3K9me2, H3K27me3, H3K36me3, H3K79me1, but level acetylated (ac) H4 was relatively stable sperm head fractions, as demonstrated by analysis. Sperm heads with lower P2...
ACE2 was observed as the cell surface receptor of SARS-CoV-2 virus. Interestingly, we also found positivity inside nucleus. The levels changed during differentiation and aging varied in distinct types. We depletion aortas female mice, similarly, caused decrease kidneys. Compared with that heart, brain kidneys, level lowest mouse lungs. In mice exposed to nicotine, not olfactory bulbs but lungs, upregulated females downregulated males. These observations indicate gender-dependent properties...
It has become evident that epitranscriptome events, mediated by specific enzymes, regulate gene expression and, subsequently, cell differentiation processes. We show methyltransferase-like proteins METTL3/METTL14 and N6-adenosine methylation (m6A) in RNAs are homogeneously distributed embryonic hearts, histone deacetylase (HDAC) inhibitors valproic acid Trichostatin A (TSA) up-regulate proteins. The levels of METTL3 mouse adult isolated from male female animals, were lower the aorta...
Genome duplication is safeguarded by constantly adjusting the activity of replicative CMG (CDC45-MCM2-7-GINS) helicase. However, minichromosome maintenance proteins (MCMs)-the structural core helicase-have never been visualized at sites DNA synthesis inside a cell (the so-called MCM paradox). Here, we solve this conundrum showing that anti-MCM antibodies primarily detect inactive MCMs. Upon conversion MCMs to CMGs, factors are required for replisome bind scaffold and block antibody binding...
In this work, we have studied the structural and functional linkage between lamin A/C, nuclear actin, organization of chromosome territories (CTs) in mammary carcinoma MCF-7 cells. Selective down-regulation A/C expression led to disruption perinuclear layer disorganization lamin-bound emerin complexes at inner membrane. The silencing resulted a decrease volume surface area territories, especially chromosomes with high heterochromatin content. Inhibition actin polymerization relaxation...
Chromosomal rearrangements and copy number variation are frequently observed in cancer cells, including multiple myeloma (MM). Karyotypic abnormalities seen MM cells correlate with the disease stage drug responses. Here, we investigate nuclear arrangement of 1q21 region; amplification this region is an important diagnostic prognostic marker MM. We examined lymphoblastoid cell line CD138- ARH-77, CD138+ MOLP-8 bone marrow fraction patients diagnosed In experimental system, that...
We studied how deficiency in lamins A/C and lamina-associated polypeptide 2α (Lap2α) affects DNA repair after irradiation. A-type Lap2α were not recruited to local lesions did accumulate γ-irradiation-induced foci (IRIF), as it is generally observed for well-known marker of lesions, 53BP1 protein. At micro-irradiated chromatin lmna double knockout (dn) dn cells, protein levels reduced, compared locally irradiated wild-type counterpart. Decreased we also whole populations by UV light....
Cell differentiation into cardiomyocytes requires activation of differentiation-specific genes and epigenetic factors that contribute to these physiological processes. This study is focused on the in vitro mouse embryonic stem cells (mESCs) induced cardiomyocytes. The effects clinically promising inhibitors histone deacetylases (HDACi) mESC cardiomyogenesis explanted hearts were also analyzed. HDAC1 depletion caused early beating compared with those wild-type (wt) counterpart. Moreover,...
Effect of electromagnetic low frequency fields was studied on mice. We analyzed level protein in brain mouse. The levels c-Jun and c-Fos brains were measured using Western-blot techniques. Female male laboratory mice exposed for 4 days to magnetic field (Bm = 2 mT, f 50 Hz). exposure took place cylindrical coil at temperature. After the experiment they sacrificed different parts estimated. expression not affected by other hand decreased after exposure. results did depend sex