A5058731927- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- Neurobiology and Insect Physiology Research
- Visual perception and processing mechanisms
- Retinal Development and Disorders
- Functional Brain Connectivity Studies
- Neuroscience and Neural Engineering
- Photoacoustic and Ultrasonic Imaging
- Memory and Neural Mechanisms
- Olfactory and Sensory Function Studies
- Optical Imaging and Spectroscopy Techniques
- Genetic Syndromes and Imprinting
- Zebrafish Biomedical Research Applications
- Neural Networks and Applications
- Advanced Memory and Neural Computing
- Domain Adaptation and Few-Shot Learning
- Gaussian Processes and Bayesian Inference
- Force Microscopy Techniques and Applications
- Epigenetics and DNA Methylation
- Advanced Graph Neural Networks
- Neuroinflammation and Neurodegeneration Mechanisms
- Machine Learning in Materials Science
University of California, Santa Barbara
2018-2025
University of North Carolina at Chapel Hill
2012-2025
Center for Neurosciences
2016-2018
North Carolina State University
2016
University of North Carolina Health Care
2016
University College London
2007-2013
Indiana University School of Medicine
2012
Wolfson Foundation
2011
University of California, Los Angeles
2003-2010
Institute of Cell Biology and Neurobiology
2008
Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, offers subcellular resolution over a large ~25 mm
A diverse array of interneuron types regulates activity in the mammalian neocortex. Two most abundant are fast-spiking, parvalbumin-positive (PV + ) interneurons, which target axosomatic region pyramidal cells, and somatostatin-positive (SOM dendrites. Recent work has focused on influence PV SOM interneurons cells. However, connections among poorly understood could play an important role cortical circuitry, since their interactions may alter net cell output. We used optogenetic approach to...
Abstract Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue has transformed neural imaging, but applying this technique vivo be limited by the mechanical optical constraints of conventional objectives. Short working distance objectives collide with compact surgical windows or other instrumentation preclude imaging. Here we present an ultra-long (20 mm) air objective called Cousa objective. It is optimized for performance across multiphoton...
At small central synapses, efficient turnover of vesicles is crucial for stimulus-driven transmission, but how the structure this recycling pool relates to its functional role remains unclear. Here we characterize organizational principles at native hippocampal synapses with nanoscale resolution using fluorescent dye labeling and electron microscopy. We show that broadly scales magnitude total vesicle pool, average size (∼45 vesicles), highly variable, regulated by CDK5/calcineurin activity....
Neurophotonics was launched in 2014 coinciding with the launch of BRAIN Initiative focused on development technologies for advancement neuroscience. For last seven years, Neurophotonics' agenda has been well aligned this focus neurotechnologies featuring new optical methods and tools applicable to brain studies. While 2.0 is pivoting towards applications these novel quest understand brain, article we review an extensive diverse toolkit explore function that have emerged from related...
Dendritic spines are a morphological feature of the majority excitatory synapses in mammalian neocortex and motile structures with shapes lifetimes that change throughout development. Proper cortical development function, including contributions to learning memory formation, require appropriate experience-dependent dendritic spine remodeling. abnormalities have been reported for many neurodevelopmental disorders, Angelman syndrome (AS), which is caused by loss maternally inherited UBE3A...
Apoptosis plays an essential role during brain development, yet the precise mechanism by which this pathway is regulated in remains unknown. In particular, mammalian cells are known to express multiple anti-apoptotic Bcl-2 family proteins. However, of developing could also exist a primed state loss single protein sufficient trigger apoptosis. Here, we examined critical Bcl-xL, protein, development. Using conditional knock-out mice Bcl-xL deleted neural progenitor (Bcl-xL(Emx1-Cre)), show...
Global motion perception is a function of higher, or extrastriate, visual system circuitry. These circuits can be engaged in visually driven navigation, behavior at which mice are adept. However, the properties global unclear. Therefore, we developed touchscreen-based, two-alternative forced choice (2AFC) task to explore detection using random dot kinematograms (RDK). Performance data was used compute coherence thresholds for perception. The touchscreen-based allowed parallel training and...
Abstract Understanding brain function requires monitoring local and global dynamics. Two-photon imaging of the across mesoscopic scales has presented trade-offs between area acquisition speed. We describe a flexible cellular resolution two-photon microscope capable simultaneous video rate four independently targetable regions spanning an approximate five-millimeter field view. With this system, we demonstrate ability to measure calcium activity mouse sensorimotor cortex at behaviorally...
Many types of neurons fire spontaneously because the activity pacemaking ion channels. Although endogenous firing can serve as a persistent signal to downstream targets, little attention has been paid factors that might modulate such intrinsic electrical activity. We tested for modulation spontaneous Purkinje in cerebellar slices under conditions which principal synaptic inputs were blocked. Loose-patch recordings from single show sustained (>40 min) increases rate be triggered by activation...
The mouse visual cortex contains interconnected higher areas, but their functional specializations are unclear. Here, we used a data-driven approach to examine the representations of complex stimuli by L2/3 neurons across measured using large-field-of-view two-photon calcium imaging. Using specialized stimuli, found fidelity texture in area LM, compared AL. Complementarily, motion AL, LM. We also observed this segregation information response naturalistic videos. Finally, explored how...
The hippocampus consists of a stereotyped neuronal circuit repeated along the septal-temporal axis. This transverse contains distinct subfields with connectivity that support crucial cognitive processes, including episodic and spatial memory. However, comprehensive measurements across hippocampal in vivo are intractable existing techniques. Here, we developed an approach for two-photon imaging plane awake mice via implanted glass microperiscopes, allowing optical access to major dendritic...
The posterior parietal cortex ( PPC ) in mice has various functions including multisensory integration 1–3 , vision-guided behaviors 4–6 working memory 7–13 and posture control 14,15 . However, an integrated understanding of these their cortical localizations around the higher visual areas HVAs ), not been completely elucidated. Here we simultaneously imaged activity thousands neurons within a 3 x mm 2 field-of-view, eight PPC, during behavior with two-photon mesoscope 16 Mice performed both...
The posterior parietal cortex ( PPC ) in mice has various functions including multisensory integration 1–3 , vision-guided behaviors 4–6 working memory 7–13 and posture control 14,15 . However, an integrated understanding of these their cortical localizations around the higher visual areas HVAs ), not been completely elucidated. Here we simultaneously imaged activity thousands neurons within a 3 x mm 2 field-of-view, eight PPC, during behavior with two-photon mesoscope 16 Mice performed both...