A5044874332- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Optical Imaging and Spectroscopy Techniques
- Optical Coherence Tomography Applications
- Advanced Neuroimaging Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Photoreceptor and optogenetics research
- Advanced Electron Microscopy Techniques and Applications
- Image Processing Techniques and Applications
- Anatomy and Medical Technology
- EEG and Brain-Computer Interfaces
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced MRI Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Functional Brain Connectivity Studies
- Tissue Engineering and Regenerative Medicine
- Neurological Disease Mechanisms and Treatments
- Molecular Biology Techniques and Applications
- Digital Holography and Microscopy
- Neuroscience and Neuropharmacology Research
- Elasticity and Material Modeling
- Cellular Mechanics and Interactions
- Nicotinic Acetylcholine Receptors Study
- Cardiac electrophysiology and arrhythmias
University of Florence
2015-2024
National Research Council
2019-2024
European Theoretical Spectroscopy Facility
2013-2024
National Institute of Optics
2022-2024
Instituto de Óptica "Daza de Valdés"
2022-2024
National Academies of Sciences, Engineering, and Medicine
2022-2024
At Bristol
2023
Naples Anesthesia & Physician Associates
2022
Centre Hospitalier de Lens
2018-2021
WWF Italia
2019
Extensive mapping of neuronal connections in the central nervous system requires high-throughput um-scale imaging large volumes. In recent years, different approaches have been developed to overcome limitations due tissue light scattering. These methods are generally improve performance a specific modality, thus limiting comprehensive neuroanatomical exploration by multimodal optical techniques. Here, we introduce versatile brain clearing agent (2,2'-thiodiethanol; TDE) suitable for various...
Every optical imaging technique is limited in its penetration depth by scattering occurring biological tissues. Possible solutions to overcome this problem consist of limiting the detrimental effects reducing inhomogeneities within sample. This can be achieved either using physical methods (such as refractive index matching solutions) or chemical removal scatterers), based on tissue transformation protocols. review provides an overview current state-of-the-art used for both ex-vivo and...
The distinct organization of the brain's vascular network ensures that it is adequately supplied with oxygen and nutrients. However, despite this fundamental role, a detailed reconstruction brain-wide vasculature at capillary level remains elusive, due to insufficient image quality using best available techniques. Here, we demonstrate novel approach improves demarcation by combining CLARITY staining can fill entire blood vessel lumen imaging light-sheet fluorescence microscopy. This method...
Scientific research on the impact of microplastics (MPs) in terrestrial systems is still emerging, but it has confirmed adverse health effects organisms exposed to plastics. Although recent studies have shown toxicological individual MPs polymers honey bees, different polymer combinations cognitive and behavioural performance remain unknown. To fill this knowledge gap, we investigated oral exposure spherical brain accumulation bee Apis mellifera. We evaluated acute toxicity, after a two-day...
Abstract In recent years, brain research has indisputably entered a new epoch, driven by substantial methodological advances and digitally enabled data integration modelling at multiple scales—from molecules to the whole brain. Major are emerging intersection of neuroscience with technology computing. This science combines high-quality research, across scales, culture multidisciplinary large-scale collaboration, translation into applications. As pioneered in Europe’s Human Brain Project...
Objective. Approximately 5% of women suffer from recurrent vulvovaginal candidiasis (RVVC). It has been hypothesized that genetic factors play an important role in the susceptibility to RVVC. The aim this study was assess effect variants genes encoding for Pattern Recognition Receptors (PRRs) on Study design. For study, 119 RVVC patients and 263 healthy controls were recruited. Prevalence polymorphisms five PRRs involved recognition Candida investigated controls. In silico functional studies...
The combination of optical tissue transparency with immunofluorescence allows the molecular characterization biological tissues in 3D. However, adult human organs are particularly challenging to become transparent because autofluorescence contributions aged tissues. To meet this challenge, we optimized SHORT (SWITCH-H
Neurophotonics was launched in 2014 coinciding with the launch of BRAIN Initiative focused on development technologies for advancement neuroscience. For last seven years, Neurophotonics' agenda has been well aligned this focus neurotechnologies featuring new optical methods and tools applicable to brain studies. While 2.0 is pivoting towards applications these novel quest understand brain, article we review an extensive diverse toolkit explore function that have emerged from related...
The heart is innervated by a dense sympathetic neuron network which, in the short term, controls chronotropy and inotropy and, long regulates cardiomyocyte size. Acute neurogenic control of rate achieved locally through direct neuro-cardiac coupling at specific junctional sites (neuro-cardiac junctions). ventricular topology well-defined characteristic for each mammalian species. In present study, we used cell size regulation to determine whether long-term modulation cardiac structure via...
Analyzing the structure of neuronal fibers with single axon resolution in large volumes is a challenge connectomics. Different technologies try to address this goal; however, they are limited either by ineffective labeling or achievable resolution. The possibility discriminating between different adjacent myelinated axons gives opportunity providing more information about fiber composition and architecture within specific area. Here, we propose MAGIC (Myelin Autofluorescence imaging Glycerol...
Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct reliable cell census the frontal lobe human cerebral cortex at micrometer resolution magnetic resonance imaging (MRI)-referenced system using innovative and analysis methodologies. MRI establishes macroscopic reference coordinate laminar cytoarchitectural boundaries. Cell counting is obtained with digital stereological approach on 3D reconstruction cellular from custom-made...
Unraveling the structure and function of brain requires a detailed knowledge about neuronal connections, i.e., spatial architecture nerve fibers. One most powerful histological methods to reconstruct three-dimensional fiber pathways is 3D-polarized light imaging (3D-PLI). The technique measures birefringence sections derives orientations whole human with micrometer resolution. However, yields only single orientation for each measured tissue voxel even if it composed fibers different...
Although neuronal density analysis on human brain slices is available from stereological studies, data the spatial distribution of neurons in 3D are still missing. Since organization very inhomogeneous cerebral cortex, it critical to map all a given volume rather than relying sparse sampling methods. To achieve this goal, we implement new tissue transformation protocol clear and label tissues exploit high-resolution optical sectioning two-photon fluorescence microscopy perform mesoscopic...
Fear responses are functionally adaptive behaviors that strengthened as memories. Indeed, detailed knowledge of the neural circuitry modulating fear memory could be turning point for comprehension this emotion and its pathological states. A comprehensive understanding circuits mediating encoding, consolidation, retrieval presents fundamental technological challenge analyzing activity in entire brain with single-neuron resolution. In context, we develop brain-wide neuron quantification...
Accurate labeling of specific layers in the human cerebral cortex is crucial for advancing our understanding neurodevelopmental and neurodegenerative disorders. Building on recent advancements ultra-high-resolution ex vivo MRI, we present a novel semi-supervised segmentation model capable identifying supragranular infragranular MRI with unprecedented precision. On dataset consisting 17 whole-hemisphere scans at 120 $\mu $m, propose Multi-resolution U-Nets framework that integrates global...
Interleukin (IL)-32 is an intracellular proinflammatory mediator that strongly modulates the inflammatory reaction. Recent studies have suggested involvement of IL-32 in pathogenesis malignancies. We aimed to assess whether a known germ-line polymorphism IL32 promoter expression, and it influences susceptibility and/or outcome epithelial cell–derived thyroid carcinoma (TC). In this study, genotype was assessed 139 TC patients 138 healthy controls correlated with clinical outcome....
Comprehensive mapping and quantification of neuronal projections in the central nervous system requires high-throughput imaging large volumes with microscopic resolution. To this end, we have developed a confocal light-sheet microscope that has been optimized for three-dimensional (3-D) structurally intact clarified whole-mount mouse brains. We describe optical electromechanical arrangement give details on organization management software. The software orchestrates all components microscope,...
3D reconstruction of human brain volumes at high resolution is now possible thanks to advancements in tissue clearing methods and fluorescence microscopy techniques. Analyzing the massive data produced with these approaches requires automatic able perform fast accurate cell counting localization. Recent advances deep learning have enabled development various tools for segmentation. However, quantification neurons presents specific challenges, such as pixel intensity variability,...