A5070387513- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Retinal Diseases and Treatments
- Advanced Fluorescence Microscopy Techniques
- Retinal and Macular Surgery
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Non-Invasive Vital Sign Monitoring
- Ocular and Laser Science Research
- Retinal Development and Disorders
- Coronary Interventions and Diagnostics
- Retinal and Optic Conditions
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced MRI Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiovascular Health and Disease Prevention
- Neurological Disease Mechanisms and Treatments
- Analytical Chemistry and Sensors
- Cerebrovascular and Carotid Artery Diseases
- Hemodynamic Monitoring and Therapy
- Photoreceptor and optogenetics research
- Angiogenesis and VEGF in Cancer
- Barrier Structure and Function Studies
- Thermoregulation and physiological responses
NYU Langone Health
2021-2025
University of California System
2024
Hinge Health
2021-2024
New York University
2024
Intuit (United States)
2024
University of California, Davis
2014-2023
Athinoula A. Martinos Center for Biomedical Imaging
2009-2022
Massachusetts General Hospital
2009-2022
Tufts University
2022
Virginia Tech
2018-2020
Ultrahigh-resolution optical coherence tomography uses broadband light sources to achieve axial image resolutions on the few micron scale. Fourier domain detection methods enable more than an order of magnitude increase in imaging speed and sensitivity, thus overcoming sensitivity limitations inherent ultrahigh-resolution OCT using standard time detection. also provide direct access spectrum signal. This enables automatic numerical dispersion compensation, a key factor achieving ultrahigh...
We demonstrate ultrahigh speed spectral / Fourier domain optical coherence tomography (OCT) using an CMOS line scan camera at rates of 70,000 - 312,500 axial scans per second. Several design configurations are characterized to illustrate trade-offs between acquisition speed, resolution, imaging range, sensitivity and roll-off performance. Ultrahigh resolution OCT with 2.5 3.0 micron image is demonstrated approximately 100,000 A high spectrometer improves range performance, trading off...
This report is the second part of a comprehensive two-part series aimed at reviewing an extensive and diverse toolkit novel methods to explore brain health function. While first focused on neurophotonic tools mostly applicable animal studies, here, we highlight optical spectroscopy imaging relevant noninvasive human studies. We outline current state-of-the-art technologies software advances, most recent impact these neuroscience clinical applications, identify areas where innovation needed,...
It has been shown that light speckle fluctuations provide a means for noninvasive measurements of cerebral blood flow index (CBFi). While conventional Diffuse Correlation Spectroscopy (DCS) provides marginal brain sensitivity CBFi in adult humans, new techniques have recently emerged to improve diffuse throughput and thus, sensitivity. Here we further optimize one such approach, interferometric diffusing wave spectroscopy (iDWS), with respect number independent channels, camera duty cycle...
purpose. To visualize, quantitatively assess, and interpret outer retinal morphology by using high-speed, ultrahigh-resolution (UHR) OCT. methods. Retinal imaging was performed in the ophthalmic clinic a cross-section of 43 normal subjects with 3.5-μm, axial-resolution, UHR OCT prototype instrument, radial scan pattern (24 images, 1500 axial scans). Outer layers were automatically segmented measured. High-definition 2.8-μm research to visualize finer features retina. results. Quantitative...
To demonstrate ultrahigh-speed optical coherence tomography (OCT) imaging of the retina and optic nerve head at 249,000 axial scans per second a wavelength 1060 nm. investigate methods for visualization retina, choroid, using high-density sampling enabled by improved speed.A swept-source OCT retinal system operating speed was developed. Imaging were performed. Display such as speckle reduction, slicing along arbitrary planes, en face reflectance from specific layers, image compounding...
Absolute measurements of cerebral blood flow (CBF) are an important endpoint in studies pathophysiology. Currently no accepted method exists for vivo longitudinal monitoring CBF with high resolution rats and mice. Using three-dimensional Doppler Optical Coherence Tomography cranial window preparations, we present methods algorithms regional the rat cortex. Towards this end, develop validate a quantitative statistical model to describe effect static tissue on velocity sensitivity. This is...
A Fourier domain mode-locked (FDML) laser at 1050 nm for ultra-high-speed optical coherence tomography (OCT) imaging of the human retina is demonstrated. Achievable performance, physical limitations, design rules, and scaling principles FDML operation component choice in this wavelength range are discussed. The fiber-based operates a sweep rate 236 kHz over 63 tuning range, with 7 mW average output power. Ultra-high-speed retinal demonstrated 236,000 axial scans per second. This represents...
In Brief Purpose: The purpose of this study was to report normal macular thickness measurements and assess reproducibility retinal acquired by a time-domain optical coherence tomography (OCT) (Stratus, Carl Zeiss Meditec, Inc., Dublin, CA) three commercially available spectral/Fourier domain OCT instruments (Cirrus HD-OCT, Inc.; RTVue-100, Optovue, Fremont, CA; 3D OCT-1000, Topcon, Paramus, NJ). Methods: Forty randomly selected eyes 40 normal, healthy volunteers were imaged. Subjects scanned...
In vivo imaging of cerebral tissue oxygenation is important in defining healthy physiology and pathological departures associated with disease. We used a recently developed two-photon microscopy method, based on novel phosphorescent nanoprobe, to image the rat primary sensory cortex response stimulation. Our measurements showed that stimulus-evoked increase pO 2 depended baseline level. particular, during sustained stimulation, steady-state at low-baseline locations remained baseline,...
To demonstrate high-speed, ultrahigh-resolution optical coherence tomography (OCT) for noninvasive, in vivo, three-dimensional imaging of the retina rat and mouse models.A OCT system using spectral, or Fourier domain, detection has been developed small animal retinal imaging. Imaging is performed with a contact lens postobjective scanning. An axial image resolution 2.8 mum achieved spectrally broadband superluminescent diode light source bandwidth approximately 150 nm at 900-nm center...
We describe methods and algorithms for rapid volumetric imaging of cortical vasculature with optical coherence tomography (OCT). By optimizing system design, scanning protocols, visualization capillary flow, comprehensive the surface pial bed is performed in approximately 12 s. during hypercapnia comparing simultaneous CCD imaging, sources contrast OCT angiography are investigated.
In vivo optical microscopic imaging techniques have recently emerged as important tools for the study of neurobiological development and pathophysiology. particular, two-photon microscopy has proved to be a robust highly flexible method in scattering tissue. However, typically requires extrinsic dyes or contrast agents, depths are limited few hundred microns. Here we demonstrate Optical Coherence Microscopy (OCM) neuronal cell bodies cortical myelination up ~1.3 mm rat neocortex. Imaging...
TO DATE, TWO MAIN CATEGORIES OF OCT TECHNIQUES HAVE BEEN DESCRIBED FOR IMAGING HEMODYNAMICS: Doppler and angiography. can measure axial velocity profiles flow in arteries veins, while angiography determine vascular morphology, tone, presence or absence of red blood cell (RBC) perfusion. However, neither method quantify RBC capillaries, where is typically transverse to the probe beam single-file. Here, we describe new methods that potentially address these limitations. Firstly, a...
A spectral/Fourier domain optical coherence tomography (OCT) intravital microscope using a supercontinuum light source at 1.7 μm was developed to study subcortical structures noninvasively in the living mouse brain. The benefits of for deep tissue brain imaging are demonstrated by quantitatively comparing OCT signal attenuation characteristics cortical across visible and near-infrared wavelengths. Imaging hippocampal architecture white matter microvasculature vivo through thinned-skull,...
Quantification of chromophore concentrations in reflectance mode remains a major challenge for biomedical optics. Spectroscopic Optical Coherence Tomography (SOCT) provides depth-resolved spectroscopic information necessary quantitative analysis chromophores, like hemoglobin, but conventional SOCT methods are applicable only to well-defined specular reflections, which may be absent highly scattering biological tissue. Here, by fitting the dynamic signal spectrum OCT angiogram using forward...
Noninvasive in vivo functional optical imaging of the intact retina is demonstrated by using high-speed, ultrahigh-resolution coherence tomography (OCT). Imaging was performed with 2.8 μm resolution at a rate 24,000 axial scans per second. A white-light stimulus applied to dark-adapted rat retina, and average reflectivities from different intraretinal layers were monitored as function time. 10%-15% increase amplitude reflectance photoreceptor outer segments observed response stimulus. The...
High-speed, high-resolution optical coherence tomography (OCT) imaging of the human retina is demonstrated using a frequency-swept laser at 850 nm. A compact external cavity semiconductor design, optimized for swept-source ophthalmic OCT, described. The enables an effective 16 kHz sweep rate with >10 mm length and tuning range approximately 35 nm full width half-maximum, yielding axial resolution <7 micro m in tissue.