A5103134383- Retinal Diseases and Treatments
- RNA regulation and disease
- Metabolism and Genetic Disorders
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Mitochondrial Function and Pathology
- Blood Coagulation and Thrombosis Mechanisms
- Retinal Imaging and Analysis
- Parkinson's Disease Mechanisms and Treatments
- Advanced Proteomics Techniques and Applications
- Retinal and Macular Surgery
- Gene expression and cancer classification
- S100 Proteins and Annexins
- Cellular transport and secretion
- Bioinformatics and Genomic Networks
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Systemic Sclerosis and Related Diseases
- Genomics, phytochemicals, and oxidative stress
- Systemic Lupus Erythematosus Research
- Protein Kinase Regulation and GTPase Signaling
- Glutathione Transferases and Polymorphisms
Biogen (United States)
2014-2025
Digital Proteomics (United States)
2025
Joslin Diabetes Center
2005-2015
Proteostasis Therapeutics (United States)
2012-2015
Harvard University
2005-2014
Weifang Medical University
2005
An understanding of the diabetes-induced alterations in vitreous protein composition absence and presence proliferative diabetic retinopathy (PDR) may provide insights into factors mechanisms responsible for this disease. We have performed a comprehensive proteomic analysis comparison samples from individuals with diabetes but without (noDR) or PDR nondiabetic (NDM). Using preparative one-dimensional SDS-PAGE nano-LC/MS/MS 17 independent samples, we identified 252 proteins human vitreous....
Delayed-release dimethyl fumarate (also known as gastro-resistant fumarate), an oral therapeutic containing (DMF) the active ingredient, is currently approved for treatment of relapsing multiple sclerosis. DMF also a component in distinct mixture product with 3 different salts monoethyl (MEF), which marketed psoriasis. Previous studies have provided insight into pharmacologic properties DMF, including modulation kelch-like ECH-associated protein 1 (KEAP1), activation nuclear factor...
Parkinson disease (PD) is a neurodegenerative characterized by the accumulation of alpha-synuclein (SNCA) and other proteins in aggregates termed "Lewy Bodies" within neurons. PD has both genetic environmental risk factors, while processes leading to aberrant protein aggregation are unknown, past work points abnormal levels SNCA proteins. Although several genome-wide studies have been performed for PD, these focused on DNA sequence variants association (GWAS) RNA (microarray...
Colorectal cancer (CRC) is the third most common worldwide and has poor prognosis. To identify oncofetal proteins involved in CRC carcinogenesis, differentially expressed among fetal colorectal tissues, CRC, paired tumor-adjacent normal tissues were investigated by a two-dimensional gel electrophoresis MALDI-TOF/TOF-based proteomics approach. 42 protein spots these 22 identified MS analysis. Desmin zinc finger 829 found to be elevated tissue compared with tissue. The expression of desmin...
Mutations in LRRK2 are the most common cause of autosomal dominant Parkinson's disease, and relevance to sporadic form disease is becoming ever more apparent. It therefore essential that studies conducted improve our understanding cellular role this protein. Here we use multiple models techniques identify pathways through which mutations may lead development disease.
A widely used method for protein identification couples prefractionation of samples by one-dimensional (1D) PAGE with LC/MS/MS. We developed a new label-free quantitative algorithm combining measurements spectral counting, ion intensity, and peak area on 1D PAGE-based proteomics. This has several improvements over other algorithms: (i) Errors in detection are reduced because the retention time is based each LC/MS/MS run actual precursor m/z. (ii) Detection sensitivity increased...
This study aimed to gain insights into the dynamic proteome changes and underlying molecular mechanisms of de/remyelination in a cuprizone model, widely used preclinical model multiple sclerosis (MS). Longitudinal sampling control or cuprizone-treated mouse brains was executed at 6 time points over weeks. Data analysis included 8489 quantified proteins. Differential proteomic GO analyses revealed that 5.9% altered, including reported novel de/remyelination-relevant protein pathways. We found...
Connective tissue growth factor (CTGF), a matrix-associated protein with four distinct cytokine binding domains, has roles in vasculogenesis, wound healing responses, and fibrogenesis is upregulated fibroblasts myofibroblasts disease. Here, we investigated the role of CTGF fibrogenic cells. In mice, tissue-specific inducible overexpression by kidney pericytes had no bearing on nephrogenesis or homeostasis but exacerbated inflammation fibrosis after ureteral obstruction. These effects...
Significance It is of great importance to elucidate mechanisms underlying the loss or dysfunction synapses in neurological diseases. The concept that reduced functional disease reflect aberrant reactivation pathways mediating synapse elimination development has yielded candidate mechanisms. Here, we a role cytokine TWEAK and its receptor Fn14, previously shown be critical visual development, synaptic function adult brain. We show limiting this signaling pathway augments models Alzheimer’s...
Dimethyl fumarate (DMF) is an established oral therapy for multiple sclerosis worldwide. Although the clinical efficacy of these esters has been extensively investigated, mode action and pharmacokinetics fumarates have not fully elucidated due to their broad-spectrum reactivity complex metabolism in vivo. To better understand mechanism DMF its active metabolite, monomethyl (MMF), we designed utilized clickable probes visualize enrich probe-modified proteins. We further perform quantitative...
Abstract Summary We developed xOmicsShiny, a feature-rich R Shiny-powered application that enables biologists to fully explore omics datasets across experiments and data types, with an emphasis on uncovering biological insights at the pathway level. The merging feature ensures flexible exploration of cross-omics data, such as transcriptomics, proteomics, metabolomics, lipidomics. mapping function covers broad range databases, including WikiPathways, Reactome, KEGG pathways. In addition,...
Combining a phenotypic screen with chemoproteomics reveals CDK12 as potential therapeutic target to inhibit noncanonical NF-κB in inflammation and cancer.
Stable isotope labeling via isobaric derivatization of peptides is a universally applicable approach that enables concurrent identification and quantification proteins in different samples using tandem mass spectrometry. In this study, we evaluated the performance amine-reactive tag (TMT), available as duplex sixplex sets, with regard to their ability elucidate protein expression changes. Using rat brain tissue from two developmental time points, postnatal day 1 (p1) 45 (p45), model system,...
Fibroblasts/myofibroblasts are the key effector cells responsible for excessive extracellular matrix (ECM) deposition and fibrosis progression in both idiopathic pulmonary (IPF) systemic sclerosis (SSc) patient lungs, thus it is critical to understand transcriptomic proteomic programs underlying their fibrogenic activity. We conducted first integrative analysis of fibrotic programming these at levels gene microRNA (miRNA) expression, as well deposited ECM protein gain insights into how...
Diabetic retinopathy is the most common microvascular complication caused by diabetes mellitus and a leading cause of vision loss among working-age adults in developed countries. Understanding effects on retinal proteome may provide insights into factors mechanisms responsible for this disease. We have performed comprehensive proteomic analysis comparison retina from C57BL/6 mice with 2 months streptozotocin-induced age-matched nondiabetic control mice. To explore role angiotensin AT1...
Novel therapies and biomarkers are needed for the treatment of acute ischemic stroke (AIS). This study aimed to provide comprehensive insights into dynamic proteome changes underlying molecular mechanisms post-ischemic stroke. TMT-coupled proteomic analysis was conducted on mouse brain cortex tissue from five time points up 4 weeks poststroke in distal hypoxic-middle cerebral artery occlusion (DH-MCAO) model. We found that nearly half detected altered following stroke, but only ∼8.6% were at...
Purpose.: Retinal hemorrhages occur in a variety of sight-threatening conditions including ocular trauma, high altitude retinopathy, and chronic diseases such as diabetic hypertensive retinopathies. The goal this study is to investigate the effects blood vitreous on retinal vascular function rats.
The proteomic profile of vitreous from second-trimester human embryos and young adults was characterized using mass spectrometry analyzed for changes in protein levels that may relate to structural occurring during this time. This proteome compared previous reports confirm proteins already identified reveal novel ones.Vitreous 17 aged 14 20 weeks gestation (WG) a 12-, 14-, 15-, 28-year-old individually tandem spectrometry-based proteomics. Peptide spectral count associations with embryonic...
The excessive accumulation of extracellular matrix (ECM) in the renal tubulointerstitium is a key component chronic damage lupus nephritis (LN) and critical determinant disease progression to failure. Detection fibrosis requires biopsy therefore limited by high risks associated with an invasive procedure. This study explores whether unique LN urinary peptidome can be identified LN-specific alteration reflects underlying fibrogenic process altered ECM turnover.Urinary peptides were analyzed...