A5056576365- Genetic Neurodegenerative Diseases
- Renal and related cancers
- RNA and protein synthesis mechanisms
- NF-κB Signaling Pathways
- Cancer Cells and Metastasis
- Cellular transport and secretion
- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- RNA modifications and cancer
- Computational Drug Discovery Methods
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- Genomic variations and chromosomal abnormalities
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- RNA regulation and disease
- Chromosomal and Genetic Variations
- Angiogenesis and VEGF in Cancer
- Animal Genetics and Reproduction
- Cancer Genomics and Diagnostics
- Neurological disorders and treatments
Scholar Rock (United States)
2018-2024
Biogen (United States)
2018
Pathways Behavioral Services
2012-2016
Novartis (Switzerland)
2007-2015
Novartis Institutes for BioMedical Research
2012
Mount Sinai Hospital
2008
University Health Network
2008
University of Toronto
2008
Novartis (United States)
2006
Biopeptide (United States)
2001
The chromosome 11p13 Wilms tumor susceptibility gene WT1 appears to play a crucial role in regulating the proliferation and differentiation of nephroblasts gonadal tissue. consists 10 exons, encoding complex pattern mRNA species: four distinct transcripts are expressed, reflecting presence or absence two alternative splices. Splice I separate exon, 17 amino acids, which is inserted between proline-rich terminus zinc finger domains. II arises from use an 5' splice junction results insertion 3...
X-linked lymphoproliferative syndrome (XLP) is an inherited immunodeficiency characterized by increased susceptibility to Epstein–Barr virus (EBV). In affected males, primary EBV infection leads the uncontrolled proliferation of virus-containing B cells and reactive cytotoxic T cells, often culminating in development high-grade lymphoma. The XLP gene has been mapped chromosome band Xq25 through linkage analysis discovery patients harboring large constitutional genomic deletions. We describe...
The Wilms' tumor gene WT1 is a recessive oncogene that encodes putative transcription factor implicated in nephrogenesis during kidney development. In this report we analyze expression of the murine urogenital system. expressed non-germ-cell components testis and ovaries both young adult mice. situ mRNA hybridization studies demonstrate granulosa epithelial cells ovaries, Sertoli testis, uterine wall. addition to 3.1-kb transcript detected by Northern blotting RNA from kidney, uterus,...
We have developed a method, exon amplification, for fast and efficient isolation of coding sequences from complex mammalian genomic DNA. This method is based on the selection RNA sequences, exons, which are flanked by functional 5' 3' splice sites. Fragments cloned DNA inserted into an intron, sites human immunodeficiency virus 1 tat gene contained within plasmid pSPL1. COS-7 cells transfected with these constructs, resulting transcripts processed in vivo. Splice exons fragment paired...
Inhibiting a specific isoform of TGFβ augments the effectiveness checkpoint blockade therapy in cancer treatment.
The c-myc protooncogene has been implicated in control of growth and differentiation mammalian cells. For instance, arrest is often preceded by reduction mRNA gene transcription. To elucidate the mechanisms transcription, we have begun to characterize interaction nuclear factors with 719-base-pair (bp) regulatory domain, located 1139-421 bp upstream P1 start site mouse gene. Nuclear extracts from exponentially growing WEHI 231 murine B-lymphoma cells formed multiple complexes mobility-shift...
The human Wilms' tumor predisposition gene, WT1, is a Cys-His zinc finger polypeptide which appears to be transcription factor controlling gene expression during embryonic kidney development. In order analyze the role of WT1 in nephroblast differentiation, we have isolated murine homolog WT1. An extremely high level amino acid sequence conservation (greater than 95%) extends throughout all regions predicted mouse and polypeptides. Two alternative splices within transcript been conserved...
Heat shock protein 90 (HSP90) is a key component of multichaperone complex involved in the post-translational folding large number client proteins, many which play essential roles tumorigenesis. HSP90 has emerged recent years as promising new target for anticancer therapies.The concentrations inhibitor NVP-AUY922 required to reduce cell numbers by 50% (GI50 values) were established panel breast cancer lines and patient-derived human tumors. To investigate properties compound vivo,...
Spinal muscular atrophy (SMA) is a neuromuscular disease characterized by loss of α-motor neurons, leading to profound skeletal muscle atrophy. Patients also suffer from decreased bone mineral density and increased fracture risk. The majority treatments for SMA, approved or in clinic trials, focus on addressing the underlying cause disease, insufficient production full-length SMN protein. While restoration has resulted improvements functional measures, significant deficits remain both mice...
Treatment of immature murine B lymphocytes with an antiserum against their surface immunoglobulin (sIg)M results in cell death via apoptosis. The WEHI 231 line (IgM, kappa) has been used extensively as a model for this anti-Ig receptor-mediated Anti-sIg treatment cells causes early, transient increase the levels c-myc messenger RNA and gene transcription, followed by rapid decline below control values. Given evidence role promoting apoptosis, we have characterized nature kinetics changes...