Ultrahigh-resolution optical coherence tomography uses broadband light sources to achieve axial image resolutions on the few micron scale. Fourier domain detection methods enable more than an order of magnitude increase in imaging speed and sensitivity, thus overcoming sensitivity limitations inherent ultrahigh-resolution OCT using standard time detection. also provide direct access spectrum signal. This enables automatic numerical dispersion compensation, a key factor achieving ultrahigh...

We demonstrate a new technique for frequency-swept laser operation--Fourier domain mode locking (FDML)--and its application swept-source optical coherence tomography (OCT) imaging. FDML is analogous to active short pulse generation, except that the spectrum rather than amplitude of light field modulated. High-speed, narrowband frequency sweeps are generated with repetition period equal fundamental or harmonic cavity round-trip time. An constructed using long fiber ring cavity, semiconductor...

The <i>Journal of Biomedical Optics</i> (JBO) is a Gold Open Access journal that publishes peer-reviewed papers on the use novel optical systems and techniques for improved health care biomedical research.

We demonstrate a high-speed, frequency swept, 1300 nm laser source for domain reflectometry and OCT with Fourier domain/swept-source detection. The uses fiber coupled, semiconductor amplifier tunable Fabry-Perot filter. present scaling principles which predict the maximum sweep speed trade offs in output power, noise instantaneous linewidth performance. use of an amplification stage increasing power spectral shaping is discussed detail. generates ~45 mW peak at 20 kHz rates tuning range ~120...

Optical coherence tomography (OCT) has become one of the most successful optical technologies implemented in medicine and clinical practice mostly due to possibility non-invasive non-contact imaging by detecting back-scattered light. OCT gone through a tremendous development over past 25 years. From its initial inception 1991 [Science254, 1178 (1991)] it an indispensable medical technology ophthalmology. Also fields like cardiology gastro-enterology is envisioned standard care. A key...

We demonstrate a new implementation of complex spectral optical coherence tomography (OCT) in biomedical imaging. By reconstruction both amplitude and phase we are able to use the negative positive path differences get images objects considerable thickness. An accompanying reduction coherent noise improves quality images. The property OCT that permits measurement range be increased simultaneous systems was not described previously. To show potential this technique measured an anterior...

purpose. To visualize, quantitatively assess, and interpret outer retinal morphology by using high-speed, ultrahigh-resolution (UHR) OCT. methods. Retinal imaging was performed in the ophthalmic clinic a cross-section of 43 normal subjects with 3.5-μm, axial-resolution, UHR OCT prototype instrument, radial scan pattern (24 images, 1500 axial scans). Outer layers were automatically segmented measured. High-definition 2.8-μm research to visualize finer features retina. results. Quantitative...

A new method of measurement that essentially combines Fourier-domain optical coherence tomography with spectroscopy is introduced. By use a windowed Fourier transform it possible to obtain, in addition the object structure, spectroscopic information such as absorption properties materials. The feasibility this for performing depth-resolved demonstrated glass filter plate. results are compared theoretically calculated spectra by well-known spectral characteristics light source and

An improved spectral optical coherence tomography technique is used to obtain cross-sectional ophthalmic images at an exposure time of 64&#x03BC;s per A-scan. This method allows real-time as well static tomograms be recorded in vivo.

We present an application of in vivo anterior segment imaging the human eye with ultrahigh speed swept source OCT instrument. For this purpose, a dedicated system was designed and constructed. This instrument enables axial zooming by automatic reconfiguration spectral sweep range; enhanced range mode entire while high resolution provides detailed morphological information chamber angle cornea. The 200,000 lines/s sampling density three-dimensional cornea 250 ms promising future applications...

We demonstrate high resolution, three-dimensional OCT imaging with a speed, frequency swept 1300 nm laser source. A new external cavity semiconductor design, optimized for application to source OCT, is discussed. The design of the enables adjustment an internal spectral filter change bandwidth and provides robust bulk optics design. generates ~30 mW instantaneous peak power at effective 16 kHz sweep rate tuning range ~133 full width. In domain reflectometry applications, 109 dB sensitivity...

We describe a new ultrahigh speed Spectral OCT instrument making use of CMOS camera and demonstrate high quality in vivo imaging the anterior segment human eye. The flexibility designed system allows wide range protocols. Two- three-dimensional images cornea, chamber crystalline lens are presented. A acquisition rate, up to 135,000 A-scans/second enables reconstruction during lenticular accommodation, blinking pupillary reaction light stimulus. tomographic real time dynamics accommodation...

To demonstrate high-speed, ultrahigh-resolution optical coherence tomography (OCT) for noninvasive, in vivo, three-dimensional imaging of the retina rat and mouse models.A OCT system using spectral, or Fourier domain, detection has been developed small animal retinal imaging. Imaging is performed with a contact lens postobjective scanning. An axial image resolution 2.8 mum achieved spectrally broadband superluminescent diode light source bandwidth approximately 150 nm at 900-nm center...

We propose a modified method of acquisition and analysis Spectral Optical Coherence Tomography (SOCT) data to provide information about flow velocities. The idea behind this is acquire set SOCT spectral fringes dependent on time followed by numerical using two independent Fourier transformations performed in optical frequency domains. Therefore, we calling as joint Time domain (joint STdOCT). velocities obtained STdOCT are compared with the ones known, phase-resolved SOCT. observe that...

A method for three-dimensional 3-D optical distortion (refraction) correction on anterior segment Optical Coherence Tomography (OCT) images has been developed. The consists of ray tracing through the different surfaces, following denoising, segmentation Delaunay representation and application fan correction. applied theoretically to realistic computer eye models, experimentally OCT of: an artificial with a Polymethyl Methacrylate (PMMA) cornea intraocular lens (IOL), enucleated porcine eye,...

Speckle pattern, which is inherent in coherence imaging, influences significantly axial and transversal resolution of Optical Coherence Tomography (OCT) instruments. The well known speckle removal techniques are either sensitive to sample motion, require sophisticated expensive tracking systems, or involve numerical procedures. As a result, their applicability vivo real-time imaging limited. In this work, we propose average multiple A-scans collected fully controlled way reduce the contrast....

Significance This study resolves a long-standing question about the ability of humans to perceive near infrared radiation (IR) and identifies mechanism driving human IR vision. A few previous reports our expanded psychophysical studies here reveal that can detect at wavelengths longer than 1,000 nm it as visible light, finding has not received satisfactory physical explanation. We show light activates photoreceptors through nonlinear optical process. also caused photoisomerization purified...

The possibility of measuring a full Doppler flow depth profile in parallel by use frequency-domain optical coherence tomography is demonstrated. method based on local phase analysis the backscattered signal and allows for imaging bidirectional flow. frequency limit 5 kHz presented measurements set half frame rate CCD detector array. We measured 0.3‐µm microspheres suspended distilled water at controlled rates vitro human blood through 200‐µm capillary with real-time color-encoded tomogram 2–3/s.