A5060324298- Zebrafish Biomedical Research Applications
- Vestibular and auditory disorders
- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Fish biology, ecology, and behavior
- Retinal Development and Disorders
- Marine animal studies overview
- Neural dynamics and brain function
- Visual perception and processing mechanisms
- Developmental Biology and Gene Regulation
- Hemispheric Asymmetry in Neuroscience
- Neuroscience of respiration and sleep
- Planarian Biology and Electrostimulation
- Photoreceptor and optogenetics research
- Underwater Vehicles and Communication Systems
- Neurogenesis and neuroplasticity mechanisms
- Bat Biology and Ecology Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Physiological and biochemical adaptations
- Memory and Neural Mechanisms
- Ophthalmology and Eye Disorders
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience and Neural Engineering
- Marine Sponges and Natural Products
Washington University in St. Louis
2017-2024
Howard Hughes Medical Institute
2011-2022
University of Oregon
2022
New York University
2022
Northwestern University
2012-2014
University of California, San Diego
2007-2011
Salk Institute for Biological Studies
2006-2011
University of California, Irvine
2001-2004
The cerebellum funnels its entire output through a small number of presumed glutamatergic premotor projection neurons in the deep cerebellar nuclei and GABAergic that feed back to inferior olive. Here we use transgenic mice selectively expressing green fluorescent protein glycinergic demonstrate many medial (fastigial) are fact glycinergic, not as previously thought. These exhibit similar firing properties neighboring receive direct input from both Purkinje cells excitatory fibers....
Locomotion requires precise control of spinal networks. In tetrapods and bipeds, dynamic regulation locomotion is simplified by the modular organization limb circuits, but it not known whether their predecessors, fish axial are similarly organized. Here, we demonstrate that larval zebrafish cord contains distinct, parallel microcircuits for independent dorsal ventral musculature on each side body. During normal swimming, equally active, but, during postural correction, differentially engage...
Motor circuits develop in sequence from those governing fast movements to slow. Here we examine whether upstream sensory are organized by similar principles. Using serial-section electron microscopy larval zebrafish, generated a complete map of the gravity-sensing (utricular) system spanning inner ear brainstem. We find that both tuning and developmental organizing principles vestibular topography. Patterned rostrocaudal innervation hair cells afferents creates an anatomically inferred...
Across the nervous system, neurons with similar attributes are topographically organized. This topography reflects developmental pressures. Oddly, vestibular (balance) nuclei thought to be disorganized. By measuring activity in birthdated neurons, we revealed a functional map within central projection nucleus that stabilizes gaze larval zebrafish. We first discovered both somatic position and stimulus selectivity follow neuron birthdate. Next, electron microscopy loss-of-function assays,...
The mitogen-activated protein kinase (MAPK) pathway has been implicated recently in synaptic plasticity and memory. Here we used tail shock-induced sensitization of the tail-elicited siphon withdrawal reflex <i>Aplysia</i> to examine role MAPK three different phases We show that a specific pattern serotonin (5-HT) application produces intermediate-term long-term facilitation (ITF LTF, respectively) sensory–motor (SN–MN) synapses in<i>Aplysia</i> leads sustained activation extracellular...
The identification of neuron types within circuits is fundamental to understanding their relevance behavior. In the vestibular nuclei, several classes neurons have been defined in vivo on basis activity during behavior, but it unclear how those correspond identified slice preparations. By targeting recordings labeled transgenic mouse lines, this study reveals that continuous distribution intrinsic parameters observed medial nucleus (MVN) can be neatly subdivided into two populations neurons,...
The cerebellum influences behavior and cognition exclusively via Purkinje cell synapses onto neurons in the deep cerebellar vestibular nuclei. In contrast with rich information available about organization of cortex its synaptic inputs, relatively little is known microcircuitry postsynaptic to cells. Here we examined types microcircuits through which cells influence an oculomotor controlled by cerebellum, horizontal vestibulo-ocular reflex, involves only two eye muscles. Using a combination...
An emerging consensus from studies of axial and limb networks is that different premotor populations are required for speeds locomotion. important but unresolved issue why this occurs. Here, we perform voltage-clamp recordings motoneurons in larval zebrafish during “fictive” swimming to test the idea systematic differences biophysical properties associated with differential tuning weight timing synaptic drive, which would help explain population shifts. We find increases speed accompanied by...
The spinal cord contains a diverse array of interneurons that govern motor output. Traditionally, models circuits have emphasized the role inhibition in enforcing reciprocal alternation between left and right sides or flexors extensors. However, recent work has shown also increases coincident with excitation during contraction. Here, using larval zebrafish, we investigate V2b (Gata3+) class neurons, which contribute to flexor-extensor but are otherwise poorly understood. Using newly...
Recent studies of long-term synaptic plasticity and memory have demonstrated that the same functional endpoint, such as potentiation, can be induced through distinct signaling pathways engaged by different patterns stimulation. A critical question raised these is whether induction either converge onto a common molecular mechanism or engage cascades for maintenance plasticity. We directly examined this issue in context sensitization marine mollusk Aplysia . In system, training with single...
The induction of both synaptic plasticity and memory is thought to depend on the balance between opposing molecular regulatory factors, such as protein kinases phosphatases. Here we show that inhibition phosphatase 2B (calcineurin, CaN) facilitates intermediate-term (ITM) long-term (LTM) for tail shock-induced sensitization in Aplysia without any effect short-term memory. To identify cascade underlying improvement by CaN, examined role extracellular signal-regulated kinase...
Shared lineage has diverse effects on patterns of neuronal connectivity. In mammalian cortex, excitatory sister neurons assemble into shared microcircuits. Drosophila, in contrast, with different levels Notch expression (NotchON/NotchOFF) develop distinct identities and diverge separate circuits. Notch-differentiated have been observed vertebrate spinal cord cerebellum, but whether they integrate or circuits remains unknown. Here, we evaluate how V2a (NotchOFF)/V2b (NotchON) the zebrafish...
The larval zebrafish acquires a repertoire of vestibular-driven behaviors that aid survival early in development. These rely mostly on the utricular otolith, which senses inertial (tilt and translational) head movements. We previously characterized known central brainstem targets afferents using serial-section electron microscopy brain. Here we describe rest afferents, focusing neurons whose identities are less certain our dataset. find with commissural projections have wide range predicted...