A5083598039- Cancer Genomics and Diagnostics
- Agriculture and Rural Development Research
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Wheat and Barley Genetics and Pathology
- Multiple Sclerosis Research Studies
- Agricultural pest management studies
- Genetics and Plant Breeding
- Plant Disease Resistance and Genetics
- Pancreatic and Hepatic Oncology Research
- Horticultural and Viticultural Research
- Agriculture, Plant Science, Crop Management
- Composting and Vermicomposting Techniques
- Plant Disease Management Techniques
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- Garlic and Onion Studies
- Genetic and Environmental Crop Studies
- Crop Yield and Soil Fertility
- Plant Pathogens and Fungal Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogenic Bacteria Studies
- Organ Transplantation Techniques and Outcomes
PAX Scientific (United States)
2023
Écologie Fonctionnelle et Écotoxicologie des Agroécosystèmes
2015-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Université Paris-Saclay
2021
Berry (United States)
2020
Medical University of Białystok
2019-2020
ID Genomics (United States)
2018-2020
University of Nottingham
2017
Genomic Health (United States)
2010-2016
Seattle Cancer Care Alliance
2016
In this paper we report exploratory analyses of high‐density oligonucleotide array data from the Affymetrix GeneChip® system with objective improving upon currently used measures gene expression. Our make use three sets: a small experimental study consisting five MGU74A mouse arrays, part an extensive spike‐in conducted by Gene Logic and Wyeth's Genetics Institute involving 95 HG‐U95A human arrays; dilution 75 arrays. We display some familiar features perfect match mismatch probe (PM MM)...
High density oligonucleotide array technology is widely used in many areas of biomedical research for quantitative and highly parallel measurements gene expression. Affymetrix GeneChip arrays are the most popular. In this each typically represented by a set 11–20 pairs probes. order to obtain expression measures it necessary summarize probe level data. Using two extensive spike‐in studies dilution study, we developed tools assessing effectiveness measures. We found that performance current...
Huntington's disease (HD) pathology is well understood at a histological level but comprehensive molecular analysis of the effect in human brain has not previously been available. To elucidate phenotype HD on genome-wide scale, we compared mRNA profiles from 44 brains with those 36 unaffected controls using microarray analysis. Four regions were analyzed: caudate nucleus, cerebellum, prefrontal association cortex [Brodmann's area 9 (BA9)] and motor 4 (BA4)]. The greatest number magnitude...
Non-small-cell lung cancer (NSCLC) represents a heterogeneous group of malignancies consisting essentially adenocarcinoma (ADC) and squamous cell carcinoma (SCC). Although the diagnosis treatment ADC SCC have been greatly improved in recent decades, there is still an urgent need to identify accurate transcriptome profile associated with histological subtypes NSCLC. The present study aims key dysregulated pathways genes involved development relate them clinical traits. transcriptional changes...
Abstract Pancreatic cancer is often detected late, when curative therapies are no longer possible. Here, we present non-invasive detection of pancreatic ductal adenocarcinoma (PDAC) by 5-hydroxymethylcytosine (5hmC) changes in circulating cell free DNA from a PDAC cohort ( n = 64) comparison with non-cancer 243). Differential hydroxymethylation found thousands genes, most significantly genes related to pancreas development or function GATA4 , GATA6 PROX1 ONECUT1 MEIS2 ), and pathogenesis...
Abstract DNA 5-hydroxymethylcytosine (5hmC) modification is known to be associated with gene transcription and frequently used as a mark investigate dynamic methylation conversion during mammalian development in human diseases. However, the lack of genome-wide 5hmC profiles different tissue types impedes drawing generalized conclusions about how implicated activity specificity. To meet this need, we describe map by characterizing genomic distributions 19 tissues derived from ten organ...
Quality assessment for short oligonucleotide microarray
RNA biomarkers discovered by RT-PCR-based gene expression profiling of archival formalin-fixed paraffin-embedded (FFPE) tissue form the basis for widely used clinical diagnostic tests; however, RT-PCR is practically constrained in number transcripts that can be interrogated. We have developed and optimized RNA-Seq library chemistry as well bioinformatics biostatistical methods whole transcriptome from FFPE tissue. The accommodates low inputs sample multiplexing. These both enable rediscovery...
In this retrospective cohort analysis of all adults who were members Kaiser Permanente, Northern California, between July 1995 and June 1996 (N = 2,076,303), the authors estimated prevalence, average annual costs per person, percentage total direct medical expenditures attributable to each 25 chronic acute conditions. Ordinary least squares regression was used adjust for age, gender, comorbidities. The conditions accounted 78 percent health maintenance organization’s expense age-group....
The release of toxic oxidative free radicals induced by ischemia and reperfusion may jeopardize liver graft function. N-acetylcysteine (NAC) has shown protective effects on hypothermic warm injury in animals. NAC improves hemodynamics survival rates patients with fulminant hepatic failure. aim this study was to investigate whether intraoperative treatment would improve postoperative function transplantation. Sixty chronic end-stage disease were included a prospective randomized...
Gene expression microarray experiments are expensive to conduct and guidelines for acceptable quality control at intermediate steps before after the samples hybridised chips vague. We conducted an experiment hybridising RNA from human brain 117 U133A Affymetrix GeneChips used these data explore relationship between 4 pre-chip variables 22 post-chip outcomes measures. found that were significantly correlated with each other but this correlation was strongest measures of cRNA yield....
The aim of the study was to verify association clinical relapses and brain activity with disability progression in relapsing/remitting multiple sclerosis patients receiving disease-modifying treatments Poland. Disability defined as relapse-associated worsening (RAW), independent relapse (PIRA), MRI Activity (PIRMA). Data from Therapeutic Program Monitoring System were analyzed. Three panels identified: R0, no during treatment, R1 R2 occurrence first second year respectively. In R0 panel, we...
Gene expression profiling (GEP) from formalin-fixed paraffin-embedded (FFPE) renal allograft biopsies is a promising approach for feasibly providing molecular diagnosis of rejection. However, large-scale studies evaluating the performance models using NanoString platform data to define archetypes rejection are lacking. We tested diverse retrospective cohort over 1400 FFPE biopsy specimens, rescored according Banff 2019 criteria and representing ten 11 UNOS regions, Human Organ Transplant...
The aim of the study was to assess effectiveness disease-modifying therapies (DMTs) in relapsing-remitting multiple sclerosis (RRMS) patients treated MS centres Poland.Demographic and clinical data all Polish RRMS receiving DMTs were prospectively collected from 2014 2018 electronic files using Therapeutic Program Monitoring System (SMPT).The included 10,764 with first-line 1,042 second-line programmes. IFNβ more effectively lengthened times first relapse, disability progression, brain MRI...