A5077251923- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Organ Transplantation Techniques and Outcomes
- Immune Cell Function and Interaction
- Xenotransplantation and immune response
- Clinical Nutrition and Gastroenterology
- Organ and Tissue Transplantation Research
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Liver Disease and Transplantation
- Hematopoietic Stem Cell Transplantation
- Liver physiology and pathology
- Tissue Engineering and Regenerative Medicine
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Nutrition and Health in Aging
- COVID-19 Clinical Research Studies
- Radiation Dose and Imaging
- Immunodeficiency and Autoimmune Disorders
- Transplantation: Methods and Outcomes
- Biomedical Ethics and Regulation
- Mesenchymal stem cell research
- Congenital Anomalies and Fetal Surgery
- Herpesvirus Infections and Treatments
Columbia University
2015-2025
NewYork–Presbyterian Hospital
2023-2025
Columbia University Irving Medical Center
2014-2025
University of Minnesota System
2025
New York Hospital Queens
2023-2024
University of Minnesota Medical Center
2024
Amsterdam UMC Location University of Amsterdam
2023
Georgetown University Medical Center
2020
MedStar Georgetown University Hospital
2020
Georgetown University
2020
T cell responses to viruses are initiated and maintained in tissue sites; however, knowledge of human antiviral cells is largely derived from blood. Cytomegalovirus (CMV) persists most humans, requires immunity control, yet immune remain undefined. Here, we investigated CMV-specific cells, virus persistence CMV-associated homeostasis blood, lymphoid, mucosal secretory tissues 44 CMV seropositive 28 seronegative donors. were distinct distribution patterns, highest bone marrow (BM), or lymph...
A paradigm in transplantation states that graft-infiltrating T cells are largely non-alloreactive "bystander" cells. However, the origin and specificity of allograft over time has not been investigated detail animals or humans. Here, we use polychromatic flow cytometry high throughput TCR sequencing serial biopsies to show gut-resident cell turnover kinetics human intestinal allografts correlated with balance between intra-graft host-vs-graft (HvG) graft-vs-host (GvH) reactivities clinical...
Organ donors are sources of physiologically healthy organs and tissues for life-saving transplantation, have been recently used human immunology studies which typically confined to the sampling peripheral blood. Donors comprise a diverse population with different causes death clinical outcomes during hospitalization, effects such variations on immune parameters in blood not known. We present here coordinate analysis innate adaptive components blood, lymphoid (bone marrow, spleen, lymph...
Risks of pancreaticobiliary tissue damage secondary to electrical conduction along cannulation wires in the vicinity electrocautery have rarely been independently studied and remain mostly a theoretical issue. We aimed evaluate heat generated by commercially available guidewires setting currents used for sphincterotomy assess their safety during endoscopic procedures. tested generation from 11 common retrograde cholangiopancreatography using an electrosurgical generator combination with...
Background. Intestinal transplantation (ITx) has the highest rate of rejection among solid organ grafts. We aimed to study pathophysiology after ITx but lacked a tool for assessing cellular responses within graft. Therefore, we developed novel mixed lymphocyte reaction (MLR) assay investigate immune in Methods. samples were collected, decontaminated, and processed into single-cell suspensions from 9 swine 2 patients that underwent ITx. Debris was removed using gradient centrifugation. The...
Background. The recent availability of α1,3-galactosyltransferase knockout (GalT-KO) miniature swine has eliminated anti-Gal antibodies as the major barrier to xenotransplantation, potentially bringing this modality closer clinical application. Highly-allosensitized patients, who have poor prospects receiving a suitable cross-match negative human organ, might be first patients benefit from xenotransplantation porcine organs. However, concerns exist regarding cross-reactivity alloreactive...
Background. Unlike other solid organs, no standardized treatment algorithms exist for intestinal transplantation (ITx). We established a consortium of American ITx centers to evaluate current practices. Methods. All performing during the past 3 y were invited participate. As consortium, we generated questions and collect data from each institution. The compiled analyzed. Results. Ten participated, 211 (range, 3–46; mean 21.1). Induction regimens varied widely. Thymoglobulin was most common,...
The site of transition between tissue-resident memory (TRM) and circulating phenotypes T cells is unknown. We integrated clonotype, alloreactivity, gene expression profiles graft-repopulating recipient in the intestinal mucosa at single-cell level after human transplantation. Host-versus-graft (HvG)–reactive were mainly distributed to TRM, effector (Teff)/TRM, follicular helper compartments. RNA velocity analysis demonstrated a trajectory from TRM Teff/TRM clusters association with...
By preserving part of the native liver, auxiliary partial orthotopic liver transplantation (APOLT) provides advantage potential immunosuppression (ISP) withdrawal if recovers but has had limited acceptance, especially in United States, due to technical complications and low rates regeneration. No previous study evaluated APOLT specifically for preadolescent children with fulminant hepatic failure (FHF). This population might benefit based on greater capacity Data from 13 who underwent were...
Background and Aims: Ex vivo surgery may provide a chance at R0 resection for conventionally unresectable tumors. However, long-term outcomes have not been well documented. In this study, we analyze our 11-year to define its role. Study Design: We retrospectively analyzed 46 consecutive patients who underwent ex institution 2008–2019. Results: The types of tumors were: carcinoma (n = 20), sarcoma 20) benign low grade tumor 6). type was chosen based on location vascular involvement. most...
Objective: We herein advocate for more extensive utilization of ex vivo resection techniques otherwise unresectable liver tumors by presenting the largest collective American experience. Background: Advanced in situ and vascular reconstruction have made R0 possible tumors. Ex may further expand limits resectability but remains underutilized due to concerns about technical complexity thrombosis. However, we believe that skillset required is widespread complications less severe than widely...
In Brief Background Little is known about innate lymphoid cell (ILC) populations in the human gut, and turnover of these cells their subsets after transplantation has not been described. Methods Intestinal samples were taken from 4 isolated intestine 3 multivisceral transplant recipients at time any operative resection, such as stoma closure or revision. ILCs analyzed by flow cytometry. The target population was defined being negative for lineage markers double-positive CD45/CD127. Cells...
Introduction Intestinal transplantation (ITx) is the last remaining therapy for patients with intestinal failure once parenteral nutrition no longer an option, however its use limited by immunological complications, including high rates of rejection and morbidity associated immunosuppression, such as infection malignancy. We aimed to develop a large animal model ITx which study immune response design test tolerance induction regimens. Methods Learning from prior we developed progressively...
Introduction It is unknown how intestinal B cell populations and receptor (BCR) repertoires are established maintained over time in humans. Following transplantation (ITx), surveillance ileal mucosal biopsies provide a unique opportunity to map the dynamic establishment of recipient gut lymphocyte immunosuppressed conditions. Methods Using polychromatic flow cytometry that includes HLA allele group-specific antibodies distinguishing donor from cells along with high throughput BCR sequencing,...
Although short-term outcomes for liver transplantation have improved, patient and graft survival are limited by infection, cancer, other complications of immunosuppression. Rapid induction tolerance after would decrease these complications, improving quality life. Tolerance to kidneys, but not thoracic organs or islets, has been achieved in nonhuman primates humans through the transient donor chimerism. Since is considered be tolerogenic, we tested hypothesis that renal transplant chimerism...
Weiner J, Yamada K, Ishikawa Y, Moran S, Etter Shimizu A, Smith RN, Sachs DH. Prolonged survival of GalT‐KO swine skin on baboons. Xenotransplantation 2010; 17: 147–152. © 2010 John Wiley & Sons A/S. Abstract: Background: Allogeneic is currently the best alternative to autologous as a temporary treatment for severe burns, but it has several drawbacks. As potential alternative, we have evaluated skin, which lacks expression Gal epitope, investigate effect eliminating this epitope...
Abstract Background: Questions exist concerning the safety of bovine serum albumin-glutaraldehyde (BSAG) glue in thoracic aortic surgery, vis-à-vis embolization and pseudoaneurysm formation. We examined clinical experience with BSAG to determine if such problems were detected. Methods: studied 99 consecutive patients (25 female 74 male, age range 27 86 years) whom or similar product was used for reinforcement suture lines (n = 87 glue, 12 GRF [French] glue). selectively sparingly acute...
Background Regulatory T cells (Treg) are being explored for their tolerance-inducing capabilities. Freezing and banking Treg future use makes this strategy more clinically applicable. We aimed to devise an improved method of expanding cryopreserving maximize yield, purity, function in xenotransplantation. Methods Baboon peripheral blood mononuclear were isolated from whole blood. CD4+/CD25hi by flow cytometric sorting expanded 26 days culture with interleukin (IL)-2, anti-CD3 antibody,...