A5045986819- Organ Transplantation Techniques and Outcomes
- Clinical Nutrition and Gastroenterology
- Renal Transplantation Outcomes and Treatments
- Metabolism and Genetic Disorders
- Liver Disease and Transplantation
- Organ and Tissue Transplantation Research
- Transplantation: Methods and Outcomes
- Immunotherapy and Immune Responses
- Spinal Fractures and Fixation Techniques
- Spine and Intervertebral Disc Pathology
- Intestinal Malrotation and Obstruction Disorders
- Congenital Anomalies and Fetal Surgery
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Liver physiology and pathology
- Organ Donation and Transplantation
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Pelvic and Acetabular Injuries
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Bariatric Surgery and Outcomes
- Esophageal and GI Pathology
University of Pittsburgh
1997-2025
Children's Hospital of Pittsburgh
1997-2024
University of Pittsburgh Medical Center
1997-2024
Cleveland Clinic
1999-2018
Cleveland Clinic Lerner College of Medicine
2015
Johns Hopkins University
2007-2015
University of California, San Diego
2005-2014
Johns Hopkins Medicine
2013
La Jolla Alcohol Research
2011
Johns Hopkins Hospital
2010
Lung disease is a major cause of death in the United States, with current therapeutic approaches serving only to manage symptoms. The most common chronic and life-threatening genetic lung cystic fibrosis (CF) caused by mutations transmembrane regulator (CFTR). We have generated induced pluripotent stem cells (iPSCs) from CF patients carrying homozygous deletion F508 CFTR gene, which results defective processing cell membrane. This mutation was precisely corrected using CRISPR target...
Despite therapeutic advancement, pulmonary disease still remains a major cause of morbidity and mortality around the world. Opportunities to study human lung either in vivo or vitro are currently limited. Using induced pluripotent stem cells (iPSCs), we generated mature multiciliated functional airway epithelium. Robust multiciliogenesis occurred when notch signaling was inhibited confirmed by (i) assembly multiple pericentrin-stained centrioles at apical surface, (ii) expression...
Abstract There is evidence that donor-derived dendritic cells (DC), particularly those at a precursor/immature stage, may play role in the immune privilege of liver allografts. Underlying mechanisms are poorly understood. We have examined influence vitro generated mouse liver-derived DC progenitors (DCp) on proliferative, cytotoxic, and Th1/Th2 cytokine responses induced allogeneic T cells. Liver DCp, propagated GM-CSF from C57B10 mice (H2b), only minimal proliferation, weak cytotoxic (C3H;...
Liver transplantation has been reported in a few cases of maple syrup urine disease (MSUD), but is controversial. Many patients with approved indications for liver die before grafts are available. A 25-yr-old man MSUD underwent transplantation, and his was used as domino graft 53-yr-old hepatocellular carcinoma who had low priority on the transplant waiting list unlikely to survive until routine organ procurement. Both transplants were performed “piggy back” procedures, reconstructing caval...
Objective(s): To define the evolving role of integrative surgical management including transplantation for patients gut failure (GF). Methods: A total 500 with parenteral nutrition-dependent catastrophic and chronic GF were referred intervention particularly comprised study population. With a mean age 45 ± 17 years, 477 (95%) adults 23 (5%) children. Management strategy was guided by clinical status, splanchnic organ functions, anatomy residual gut, cause GF. Surgery performed in 462 (92%)...
Background. Unlike other solid organs, no standardized treatment algorithms exist for intestinal transplantation (ITx). We established a consortium of American ITx centers to evaluate current practices. Methods. All performing during the past 3 y were invited participate. As consortium, we generated questions and collect data from each institution. The compiled analyzed. Results. Ten participated, 211 (range, 3–46; mean 21.1). Induction regimens varied widely. Thymoglobulin was most common,...
ABSTRACT: Preclinical safety and efficacy evaluation of a novel bioartificial liver support system (BLSS) was conducted using d‐galactosamine canine failure model. The BLSS houses suspension porcine hepatocytes in hollow fiber cartridge with the on one side membrane whole blood flowing other. Porcine harvested by collagenase digestion technique were infused into incubated for 16 to 24 hours prior use. Fifteen purpose‐bred male hounds, 1–3 years old, 25–30 kg, administered lethal dose, 1.5...
Objective: Bariatric surgery (BS) is currently the most effective treatment for severe obesity. However, these weight loss procedures may result in development of gut failure (GF) with need total parenteral nutrition (TPN). This retrospective study first to address anatomic and functional spectrum BS-associated GF innovative surgical modalities restore function. Methods: Over 2 decades, 1500 adults were referred history BS 142 (9%). Of these, 131 (92%) evaluated received multidisciplinary...
Abstract Domino liver transplantation (DLT) involves transplanting from a patient with metabolic disease into end‐stage the expectation that recipient will not develop syndrome or recurrent have minimal affect. The domino donor gets deceased segment of live‐donor through organ allocation system. Waitlist mortality for exceeds morbidity associated getting disease. Between 2015 and 2017, four patients three disorders at UPMC Children's Hospital Pittsburgh underwent DLT allografts maple syrup...
Background. Liver grafts are accepted across major histocompatibility barriers in mice without immunosuppressive therapy. Potentially tolerogenic immature donor dendritic cells (DC) may play a key role this phenomenon, but recovery of purified DC from normal livers for functional analysis is inherently difficult. Administration vitro propagated to recipients different types allograft can prolong transplant survival. By contrast, marked increases liver as the result Flt3 ligand (FL)...
Recent advances in immunosuppressive regimens have decreased acute cellular rejection (ACR) rates and improved intestinal multivisceral transplant (ITx) recipient survival. We investigated the role of myeloid-derived suppressor cells (MDSCs) ITx. identified MDSCs as CD33+CD11b+ lineage(CD3/CD56/CD19)−HLA-DR−/low with 3 subsets, CD14−CD15− (e-MDSCs), CD14+CD15− (M-MDSCs), CD14−CD15+ (PMN-MDSCs), peripheral blood mononuclear (PBMCs) grafted mucosa. Total MDSC numbers increased PBMCs after ITx;...
Liver transplantation has been reported in patients with methylmalonic acidemia (MMA), but long-term outcome is controversial. Many other approved indications for liver die before donor grafts are available. A 28-year-old man MMA underwent cadaveric transplantation. His was used as a domino graft 61-year-old primary sclerosing cholangitis, who had low priority on the transplant waiting list. Surgical successful, and after both have excellent function. The patient showed substantial decrease...
Abstract Aim/Background Domino liver transplantation (DLT) using allografts from patients with metabolic disorders enhances organ utilization. Short‐ and long‐term course outcome of these can impact the decision to offer this procedure patients, especially those diseases that potentially be cured transplant. We reviewed outcomes DLT maple syrup urine disease (MSUD) in our large academic pediatric adult transplant program. Methods All receiving were analyzed retrospectively a minimum one‐year...
Background. The long-term safety and efficacy of tacrolimus in pancreas transplantation has not yet been demonstrated. observation prolonged pancreatic graft function under would indicate that any potential islet toxicity is short-lived clinically insignificant. We report herein the results patients receiving primary immunosuppression for a minimum 2 years. Methods. From July 4, 1994 until April 18, 1996, 60 received either simultaneous pancreas-kidney transplant (n=55), only (n=4), or after...
Enhanced B-cell presentation of donor alloantigen relative to HLA-mismatched reference is associated with acute cellular rejection (ACR), when expressed as a ratio called the antigen presenting index (API) in an exploratory cohort liver and intestine transplant (LT IT) recipients.